Skip to main content
SpringerLink
Log in

Regeneration of Plants from Protoplasts of Cucumis sativus L. (Cucumber)

  • Chapter
Plant Protoplasts and Genetic Engineering III

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 22))

  • 163 Accesses

Abstract

Cucumber (Cucumis sativus L.) is one of about 25 species in the genus Cucumis (family Cucurbitaceae) and is one of only a few species which is grown for human consumption. The muskmelon, C. melo L., gherkin, C. anguria L. and jelly melon (kiwano), C. metuliferus Naud. are other edible species which are cultivated to various extents in different regions of the world. Most of the wild species of Cucumis are indigenous to Africa, while C. sativus and C. sativus var. hardwickii are native to Asia; cucumber is believed to have been first domesticated in India. Cucumber fruits are used primarily for fresh market consumption or for pickling. Commercial production of this crop can be found throughout different regions of the world, both tropical and temperate, under either field conditions or in controlled environments under glasshouses. Cultivars have been developed whose agronomic characteristics can differ markedly, reflecting the different growing environments and cultural requirements for this crop. Growth and development of cucumber are generally favored by temperatures above 20°C and the plant has little to no frost tolerance.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Chee PP (1993) Transformation in cucumber (Cucumis sativus L.) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 23. Plant protoplasts and genetic engineering IV. Springer, Berlin Heidelberg New York pp 214–227.

    Google Scholar 

  • Colijn-Hooymans CM, Bouwer R, Dons JJM (1988a) Plant regeneration from cucumber protoplasts. Plant Cell Tissue Organ Cult 12:147–150.

    Article  Google Scholar 

  • Colijn-Hooymans CM, Bouwer R, Orczyk W, Dons JJM (1988b) Plant regeneration from cucumber (Cucumis sativus) protoplasts. Plant Sci 57:63–71.

    Article  Google Scholar 

  • Coutts RHA, Wood KR (1975) The isolation and culture of cucumber mesophyll protoplasts. Plant Sci Lett 4:189–193.

    Article  Google Scholar 

  • Coutts RHA, Wood KR (1977) Improved isolation and culture methods for cucumber mesophyll protoplasts. Plant Sci Lett 9:45–51.

    Article  CAS  Google Scholar 

  • Davey MR (1983) Recent developments in the culture and regeneration of plant protoplasts. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983. Birkhauser, Basel, pp 19–31.

    Google Scholar 

  • Dons JJM, Bouwer R (1986) Improving the culture of cucumber protoplasts by using an agarose-disc procedure. In: Symp Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 497–504.

    Google Scholar 

  • Durand J, Potrykus I, Donn G (1973) Plantes issues de protoplasts de Petunia. Z Pflanzenphysiol 69:26–34.

    CAS  Google Scholar 

  • Fassulliotis G (1967) Species of Cucumis resistant to the root-knot nematode Meloidogyne incognita acrita. Plant Dis Rep 51:720–723.

    Google Scholar 

  • Harada H (1973) A new method for obtaining protoplasts from mesophyll cells. Z Pflanzenphysiol 69:77–80.

    Google Scholar 

  • Jia S-R, Fu Y-Y, Lin Y (1986) Embryogenesis and plant regeneration from cotyledon protoplast culture of cucumber (Cucumis sativus L.). J Plant Physiol 124:393–398.

    CAS  Google Scholar 

  • Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca. Mol Gen Genet 150:225–230.

    Article  Google Scholar 

  • Kao KN, Michayluk MR (1989) Fusion of plant protoplasts-Techniques. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8: Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York, pp 277–288.

    Google Scholar 

  • Kho YO, Nijs APM den, Franken J (1980) Interspecific hybridization in Cucumis L. II. The crossability of species, an investigation of in vivo pollen tube growth and seed set. Euphytica 29:661–672.

    Article  Google Scholar 

  • Kim S-G, Chang J-R, Cha HC, Lee K-W (1988) Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.). Plant Cell Tissue Organ Cult 12:67–74.

    Article  Google Scholar 

  • Lu DY, Cocking EC, Davey MR, Pental D (1984) Cultural studies of protoplasts isolated from cotyledons of Cucumis sativus, Lactuca sativa and Glycine max. In: Symp Genetic manipulation in crops. IRRI, Beijing, pp 209–210.

    Google Scholar 

  • Malepszy S (1988) Cucumber (Cucumis sativus L.) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 6: Crops II. Springer, Berlin Heidelberg New York, pp 277–293.

    Google Scholar 

  • Maule A J, Boulton MI, Wood KR (1980) An improved method for the infection of cucumber leaf protoplasts with cucumber mosaic virus. Phytopathol Z 97:118–126.

    Article  CAS  Google Scholar 

  • Mclnnes E, Morgan AJ, Mulligan BJ, Davey MR (1991) Roots induced on cucumber cotyledons by the agropine Ri plasmid TR-DNA exhibit the transformed phenotype. Plant Cell Rep 9:647–650.

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.

    Article  CAS  Google Scholar 

  • Nijs APM den, Custers JBM (1990) Introducing resistance into cucumbers by interspecific hybridization. In: Bates DM, Robinson RW, Jeffrey C (eds) Biology and utilization of the Cucurbitaceae. Cornell Univ Press, pp 382-396.

    Google Scholar 

  • Orczyk W, Malepszy S (1985) In vitro culture of Cucumis sativus L. V. Stabilizing effects of glycine on leaf protoplasts. Plant Cell Rep 4:269–273.

    Article  CAS  Google Scholar 

  • Portykus I, Harms CT, Lörz H (1979) Multiple-drop assay (MDA) technique for the large-scale testing of culture media variations in hanging micro-drop cultures of single cell systems. I. The technique. Plant Sci Lett 9:231–235.

    Google Scholar 

  • Provvidenti R, Robinson RW (1974) Resistance to squash mosaic virus and watermelon mosaic virus 1 in Cucumis metuliferus. Plant Dis Rep 58:735–738.

    Google Scholar 

  • Punja ZK, Tang FA, Watkins LH (1988) Identification of resistance to root knot nematodes and virus diseases in Cucumis metuliferus and approaches to hybridization with Cucumis sativus by protoplast fusion. Phytopathology 78:1578 (Abstr).

    Google Scholar 

  • Punja ZK, Abbas N, Sarmento GG, Tang FA (1990a) Regeneration of Cucumis sativus var. sativus and C. sativus var. hardwickii, C. melo, and C. metuliferus from expiants through somatic embryogenesis and organogeneis. Plant Cell Tissue Organ Cult 21:93–102.

    Article  CAS  Google Scholar 

  • Punja ZK, Tang FA, Sarmento GG (1990b) Isolation, culture and plantlet regeneration from cotyledon and mesophyll protoplasts of two pickling cucumber (Cucumis sativus L.) genotypes. Plant Cell Rep 9:61–64.

    Article  Google Scholar 

  • Raamsdonk LWD van, Nijs APM den, Jongerius MC (1989) Meiotic analyses of Cucumis hybrids, with an evolutionary evaluation of the genus Cucumis (Cucurbitaceae). Plant Syst Evol 163:133–146.

    Article  Google Scholar 

  • Raharjo SHT, Punja ZK (1993) Plantlet regeneration from petiole expiants of the African horned cucumber, Cucumis metuliferus. Plant Cell Tissue Organ Cult. 32:169–174.

    Article  CAS  Google Scholar 

  • Sarmento GG. Alpert K, Tang FA, Punja ZK (1992) Factors influencing Agrobacterium tumefaciens-mediated transformation and expression of kanamycin resistance in pickling cucumber. Plant Cell Tissue Organ Cult 31:185–193.

    CAS  Google Scholar 

  • Shahin EA (1985) Totipotency of tomato protoplasts. Theor Appl Genet 69:235–240.

    Article  CAS  Google Scholar 

  • Shillito RD, Paszkowski J, Potrykus I (1983) Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. Plant Cell Rep 2:244–247.

    Article  CAS  Google Scholar 

  • Singh AK (1990) Cytogenetics and evolution in the Cucurbitaceae. In: Bates DM, Robinson RW, Jeffrey C (eds) Biology and utilization of the Cucurbitaceae. Cornell Univ Press, pp 10-28.

    Google Scholar 

  • Tang FA, Punja ZK (1989) Isolation and culture of protoplasts of Cucumis sativus and Cucumis metuliferus and methods for their fusion. Cucurb Gen Coop Rep 12:29–34.

    Google Scholar 

  • Trulson AJ, Shahin EA (1986) In vitro plant regeneration in the genus Cucumis. Plant Sci 47:35–43.

    Article  CAS  Google Scholar 

  • Wehner TC, Cade RM, Locy RD (1990) Cell, tissue and organ culture techniques for genetic improvement of cucurbits. In: Bates DM, Robinson RW, Jeffrey C (eds) Biology and utilization of the Cucurbitaceae. Cornell Univ Press, pp 367-381.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada, V5A 1S6

    S. H. T. Raharjo

Authors
  1. Z. K. Punja
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. H. T. Raharjo
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. A-137 New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Punja, Z.K., Raharjo, S.H.T. (1993). Regeneration of Plants from Protoplasts of Cucumis sativus L. (Cucumber). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering III. Biotechnology in Agriculture and Forestry, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78006-6_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-78006-6_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78008-0

  • Online ISBN: 978-3-642-78006-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation