Abstract
Lobelia inflata L. (Campanulaceae) is an annual plant with an erect, angular, slightly pubescent stem of up to 50 cm. It is commonly known as Indian tobacco, since it is used as a substitute for tobacco by American Indians. Several dialkylpiperidine alkaloids have been isolated from this plant (Schöpf and Kauffmann 1957). The total alkaloid content of L. inflata was found to be 0.134–0.635%, and the quantity of alkaloid varies with the culture conditions (Kalashnikov 1939).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aird ELH, Hamill JD, Rhodes MJC (1988) Cytogenetic analysis of hairy root cultures from a number of plant species transformed by Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult 15:47–57.
Davison GC, Rosen RC (1972) Lobeline and reduction of cigarette smoking. Psychol Rep 31:443–456.
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158.
Hamill JD, Parr AJ, Rhodes MJC, Robins RJ, Walton NJ (1987) New routes to plant secondary products. Bio/Technol 5:800–804.
Kalashnikov VP (1939) The contents of alkaloids in the cultivated and in the wild types of lobelia. Farmatsiya 1:20–28.
Ko KS, Ebizuka Y, Noguchi H, Sankawa U (1988) Production of secondary metabolites by hairy roots and regenerated plants transformed with Ri plasmids. Chem Pharm Bull 36:4217–4220.
Ko KS, Noguchi H, Ebizuka Y, Sankawa U (1989) Oligoside production by hairy root cultures transformed by Ri plasmids. Chem Pharm Bull 37:245–248.
Krajewska A, Szoke E, Szarvas T (1988) The influence of the new synthetic cytokinins on Lobelia inflata L. tissue culture. Herba Pol 34:27–34.
Krochmal A, Wilken L, Chien M (1972) Plant and lobeline harvest of L. inflata L. Econ Bot 26:216–220.
Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Agric Biol Chem 50:2715–2722.
Mano Y, Ohkawa H, Yamada Y (1989) Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes. Plant Sci 59:191–201.
Marshall WR (1928) Alpha lobelin as a respiratory stimulant. Arch Int Med 42:180–188.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497.
Nitsch C, Nitsch JP (1967) The induction of flowering in vitro in stem segments of Plumbago indica L. Planta (Berl.) 72:355–370.
Otten LABM, Schilperoort RA (1978) A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. Biochem Biophys Acta 527:497–500.
Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Further extention of the opine concept: Plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:204–214.
Reavill C, Walther B, Stolerman IP, Testa B (1990) Behavioural and pharmacokinetic studies on nicotine, cytisine, and lobeline. Neuropharmacology 29:619–624.
Saito K, Kaneko H, Yamazaki M, Yoshida M, Murakoshi I (1990) Stable transfer and expression of chimeric genes in licorice Glycyrrhiza uralensis, using an Ri plasmid binary vector. Plant Cell Rep 8:718–721.
Schöpf C, Kauffmann T, (1957) Über die stärker hydrophilen Nebenalkaloide aus Lobelia inflata L.; ein Beitrag zur Biogenese der Lobelia-alkaloide. Annalen der Chemie 608:88–127.
Slightom JL, Durand-Tardif M, Jouanin L, Tepfer D (1986) Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. J Biol Chem 261:108–121.
Southern EM (1975) Detection of specific sequence among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517.
Trevelyan WE, Procter DP, Harrison JS (1950) Detection of sugars on paper chromatograms. Nature 166:444–445.
Vervliet G, Holsters M, Teuchy H, Montagu M van, Schell J (1975) Characterization of different plaqueforming and defective temperate phages in Agrobacterium strains. J Gen Virol 26:33–48.
Wysokinska H (1977) Wystepowanie alkaloidow w hodowli tkankowej Lobelia inflata L. Farm Pol 33:725–727.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Tanaka, M. et al. (1993). Transformation in Lobelia inflata . In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering III. Biotechnology in Agriculture and Forestry, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78006-6_23
Download citation
DOI: https://doi.org/10.1007/978-3-642-78006-6_23
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-78008-0
Online ISBN: 978-3-642-78006-6
eBook Packages: Springer Book Archive