Regeneration of Plants from Protoplasts of Atropa belladonna L. (Deadly Nightshade)
Atropa belladonna (deadly nightshade), a member of the family Solanaceae, is a rich source of tropane alkaloids, which are antimuscaric drugs and have been used for inducing sleep, as a cure for toothache, also having hallusinigenic effects. Hyoscyamine and scopolamine are the two main tropanes. Because of the pharmaceutical interest in this plant, a considerable amount of work has been done on the in vitro culture, morphogenesis, and the production of tropane alkaloids, and the subject has been recently reviewed by Bajaj and Simola (1991). Recently, genetic transformation of Atropa belladonna through Agrobacterium rhizogenes has attracted much attention to increase production of medicinal compounds (Kamada et al. 1986; Jung and Tepffer 1987).
KeywordsSomatic Hybrid Protoplast Fusion Mesophyll Protoplast Agrobacterium Rhizogenes Tropane Alkaloid
Unable to display preview. Download preview PDF.
- Bajaj YPS (1988) Regeneration of plants from frozen (−196°C) protoplasts of Atropa belladonna L., Datura innoxia Mill and Nicotiana tabacum L. Indian J Exp Biol 26:289–292.Google Scholar
- Bajaj YPS (1989) Recent advances in the isolation and culture of protoplasts and their implications in crop improvement. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 8, Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York pp 3–22.Google Scholar
- Bajaj YPS, Simola LK (1991) Atropa belladonna L.: in vitro culture, regeneration of plants, cryopreservation, and the production of tropane alkalods. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 15, Medicinal and aromatic plants III. Springer, Berlin Heidelberg New York, pp 1–23.Google Scholar
- Bajaj YPS, Gosch G, Ottma M, Weber A, Gröbler A (1978) Production of polyploid and aneuploid plants from anthers and mesophyll protoplasts of Atropa belladonna and Nicotiana tabacum. Indian J Exp Biol 16:947–953.Google Scholar
- Cocking EC, Peberdy JF (eds) (1974) The use of protoplasts from fungi and higher plants as genetic systems — a practical handbook. Nottingham Univ.Google Scholar
- Thomas E, Street HE (1970) Organogenesis in cell suspension cultures of Atropa belladonna L. and Atropa belladonna cultivar lutea Döll. Ann Bot 34:657–669.Google Scholar