Transformation in Digitalis purpurea L. (Foxglove)
Foxglove (Digitalis purpurea L., common foxglove, D. lanata Ehrh., Grecian foxglove, and other related species) is one of the most commonly used medicinal plants in the world (Trease and Evans 1983). The dried leaves are used as a crude drug and contain a number of cardioactive glycosides, e.g., digitoxin, gitoxin, etc. A large number of reports have been published on tissue culture, regeneration, production, and biotransformation of cardenolides in Digitalis species (for review see Rücker 1988). However, only one paper has appeared on genetic transformation of D. purpurea (Saito et al. 1990a). The efficient transfer and the strong expression of foreign genes in foxglove offer promising possibility of genetic manipulation of secondary metabolism of this plant.
KeywordsChlorophyll Assimilation Sewage Gall Glycoside
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- Jafferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: b-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907.Google Scholar
- Rücker W (1988) Digitalis spp.: In vitro culture, regeneration, and the production of cardenolides and other secondary products. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 388–418.Google Scholar
- Saito K, Yamazaki M, Yamakawa K, Fujisawa S, Takamatsu S, Kawaguchi A, Murakoshi I (1989a) Lupin alkaloids in tissue culture of Sophora flavescens var. angustifolia: greening-induced production of matrine. Chem Pharm Bull 37:3001–3004.Google Scholar
- Trease GE, Evans WC (1983) Pharmacognosy, 12th edn. Bailliere Tindall, London, pp 500–509.Google Scholar
- Veiten J, Veiten L, Hain R, Schell J (1984) Isolation of a dual plant promoter fragment from the Ti plasmid of Agrobacterium tumefaciens. EMBO J 3:2723–2730.Google Scholar