Serum Neuron-Specific Enolase in Ischemic Brain Damage
Neuron-specific enolase (NSE) represents the y,y isoenzyme of the dimeric protein enolase which is a soluble enzyme of the glycolytical pathway. Physiologically, NSE is specifically present in neuronal cytoplasm and dendrites, and in cells of the amine precursor uptake and decarboxylation (APUD) cell system . Since patients suffering from APUDomas, neuroblastomas, or small cell carcinoma of the lung show elevated NSE titers in serum, NSE has been clinically established as a diagnostic and prognostic marker in such neoplasms [1, 5, 8]. However, recently obtained immunohistochemical findings of a decrease in neuronal NSE immunoreactivity in experimental animals and humans following transient cerebral ischemia suggested cytoplasmic NSE loss due to ischemia [3, 6]. Previous studies have shown intracellular compounds, especially NSE, to accumulate in the extracellular fluid and cerebrospinal fluid (CSF) after ischemia . The present study was performed in order to clarify if neuronal NSE release in ischemic brain damage is accompanied by an increase of NSE levels in serum. Therefore, serum NSE was measured at various times after experimental transient forebrain ischemia followed by histological evaluation of ischemic neuronal damage.
KeywordsIschemia Neurol Neuroblastoma Halothane Cresyl
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