Calcitonin Gene Products: Molecular Biology, Chemistry, and Actions

  • W. Born
  • J. A. Fischer
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 107)


Calcitonin was discovered by Copp et al. (1961) as a hypocalcemic hormone and the neuropeptide calcitonin gene-related peptide (CGRP) recognized through analysis of the calcitonin gene structure (Amara et al. 1982).


Medullary Thyroid Carcinoma Salmon Calcitonin Islet Amyloid Polypeptide Calcitonin Receptor Human Calcitonin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Abou-Samra A-B, Jüppner H, Force T, Freeman MW, Kong X-F, Schipani E, Urena P, Richards J, Bonventre JV, Potts JT Jr, Kronenberg HM, Segre GV (1992) Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium. Proc Natl Acad Sci 89:2732-2736PubMedCrossRefGoogle Scholar
  2. Adema GJ, Bovenberg RAL, Jansz HS, Baas PD (1988) Unusual branch point selection involved in splicing of the alternatively processed calcitonin/CGRP-I pre-mRNA. Nucleic Acids Res 16:9513-9526PubMedCrossRefGoogle Scholar
  3. Adema GJ, van Hulst KL, Baas PD (1990) Uridine branch acceptor is a cis-acting element involved in regulation of the alternative processing of calcitonin/CGRP- I pre-mRNA. Nucleic Acids Res 18:5365-5373PubMedCrossRefGoogle Scholar
  4. Ahrén B, Ekman R, Laurberg P (1989) Calcium stimulates the release of calcitonin gene-related peptide from the canine thyroid. Am J Physiol 256:E597-E599PubMedGoogle Scholar
  5. Alevizaki M, Shiraishi A, Rassool FV, Ferrier GJM, MacIntyre I, Legon S (1986) The calcitonin-like sequence of the ß CGRP gene. FEBS Lett 206:47-52PubMedCrossRefGoogle Scholar
  6. Ali-Rachedi A, Varndell IM, Facer P, Hillyard CJ, Craig RK, MacIntyre I, Polak JM (1983) Immunocytochemical localisation of katacalcin, a calcium-lowering hormone cleaved from the human calcitonin precursor. J Clin Endocrinol Metab 57:680-682PubMedCrossRefGoogle Scholar
  7. Aloia JF, Vaswani A, Kapoor A, Yeh JK, Cohn SH (1985) Treatment of osteoporosis with calcitonin, with and without growth hormone. Metabolism 34:124-129PubMedCrossRefGoogle Scholar
  8. Amara SG, Jonas V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298:240-244PubMedCrossRefGoogle Scholar
  9. Amara SG, Evans RM, Rosenfeld MG (1984) Calcitonin/calcitonin gene-related peptide transcription unit: tissue-specific expression involves selective use of alternative polyadenylation sites. Mol Cell Biol 4:2151-2160PubMedGoogle Scholar
  10. Amara SG, Arriza JL, Leff SE, Swanson LW, Evans RM, Rosenfeld MG (1985) Expression in brain of a messenger RNA encoding a novel neuropeptide homologous to calcitonin gene-related peptide. Science 229:1094-1097PubMedCrossRefGoogle Scholar
  11. Anand IS, Gurden J, Wander GS, O’Gara P, Harding SE, Ferrari R, Cornacchiari A, Panzali A, Wahi PL, Poole-Wilson PA (1991) Cardiovascular and hormonal effects of calcitonin gene-related peptide in congestive heart failure. J Am Coll Cardiol 17:208-217PubMedCrossRefGoogle Scholar
  12. Ardaillou R, Paillard F, Scraer J, Vallee G (1973) Compared kinetics of salmon and human radioiodinated calcitonin in man. Horm Metab Res 5:232-233PubMedCrossRefGoogle Scholar
  13. Arias J, Scopsi L, Fischer JA, Larsson LI (1989) Light- and electron-microscopic localization of calcitonin, calcitonin gene-related peptide, somatostatin and C- terminal gastrin/cholecystokinin immunoreactivities in rat thyroid. Histochemistry 91:265-272PubMedCrossRefGoogle Scholar
  14. Arvidsson U, Cullheim S, Ulfhake B, Hökfelt T, Terenius L (1989) Altered levels of calcitonin gene-related peptide (CGRP)-like immunoreactivity of cat lumbar motoneurons after chronic spinal cord transection. Brain Res 489:387-391PubMedCrossRefGoogle Scholar
  15. Arvidsson U, Schalling M, Cullheim S, Ulfhake B, Terenius L, Verhofstad A, Hökfelt T (1990) Evidence for coexistence between calcitonin gene-related peptide and serotonin in the bulbospinal pathway in the monkey. Brain Res 532:47-57PubMedCrossRefGoogle Scholar
  16. Avioli LV, Shieber W, Kipnis DM (1971) Role of glucagon and adrenergic receptors in thyrocalcitonin release in the dog. Endocrinology 88:1337-1340PubMedCrossRefGoogle Scholar
  17. Baron R, Neff L, Brown W, Louvard D, Courtoy PJ (1990) Selective internalization of the apical plasma membrane and rapid redistribution of lysosomal enzymes and mannose 6-phosphate receptors during osteoclast inactivation by calcitonin. J Cell Sci 97:439-447PubMedGoogle Scholar
  18. Batten TFC, Cambre ML (1989) Calcitonin gene-related peptide-like immunoreactive fibres innervating the hypothalamic inferior lobes of teleost fishes. Neurosci Lett 98:1-7PubMedCrossRefGoogle Scholar
  19. Bauerfeind P, Hof R, Hof A, Cucala M, Siegrist S, von Ritter C, Fischer JA, Blum AL (1989) Effects of hCGRP I and II on gastric blood flow and acid secretion in anesthetized rabbits. Am J Physiol 256:G145-G149PubMedGoogle Scholar
  20. Baylin SB, Höppener JWM, de Bustros A, Steenbergh PH, Lips CJM, Nelkin BD (1986) DNA méthylation patterns of the calcitonin gene in human lung cancers and lymphomas. Cancer Res 46:2917-2922PubMedGoogle Scholar
  21. Beglinger C, Born W, Hildebrand P, Ensinck JW, Burkhardt F, Fischer JA, Gyr K (1988) Calcitonin gene-related peptides I and II and calcitonin: distinct effects on gastric acid secretion in humans. Gastroenterology 95:958-965PubMedGoogle Scholar
  22. Besnard P, Jousset V, Garel J-M (1989) Additive effects of dexamethasone and calcium on the calcitonin mRNA level in adrenalectomized rats. FEBS Lett 258:293-296PubMedCrossRefGoogle Scholar
  23. Bijvoet OLM, van der Sluys Veer J, de Vries HR, van Koppen ATJ (1971) Natriuretic effect of calcitonin in man. N Engl J Med 284:681-688PubMedCrossRefGoogle Scholar
  24. Birnbaum RS, Mahoney WC, Roos BA (1986) Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line: J Biol Chem 261:699-703PubMedGoogle Scholar
  25. Body J-J, Heath H III (1983) Estimates of circulating monomeric calcitonin: physio- logical studies in normal and thyroidectomized man. J Clin Endocrinol Metab 57:897-903PubMedCrossRefGoogle Scholar
  26. Bone HG III, Catherwood BD, Deftos LJ (1983) Extraction of a substance with calcitonin-like immunoreactivity from pituitary glands of intact and thyroidec- tomized rats. Calcified Tissue Int 35:620-623CrossRefGoogle Scholar
  27. Born W, Beglinger C, Fischer JA (1991) Diagnostic relevance of the amino-terminal cleavage peptide of procalcitonin (PAS-57), calcitonin and calcitonin gene-related peptide in medullary thyroid carcinoma patients. Regul Pept 32:311-319PubMedCrossRefGoogle Scholar
  28. Bouizar Z, Fouchereau-Peron M, Taboulet J, Moukhtar MS, Milhaud G (1986) Purification and characterization of calcitonin receptors in rat kidney membranes by covalent cross-linking techniques. Eur J Biochem 155:141-147PubMedCrossRefGoogle Scholar
  29. Bouizar Z, Rostène WH, Milhaud G (1987) Down-regulation of rat kidney calcitonin receptors by salmon calcitonin infusion evidenced by autoradiography. Proc Natl Acad Sci USA 84:5125-5128PubMedCrossRefGoogle Scholar
  30. Bovenberg RAL, van de Meerendonk WPM, Baas PD, Steenbergh PH, Lips CJM, Jansz HS (1986) Model for alternative RNA processing in human calcitonin gene expression. Nucleic Acids Res 14:8785-8803PubMedCrossRefGoogle Scholar
  31. Braga P, Ferri S, Santagostino A, Olgiati VR, Pecile A (1978) Lack of opiate receptor involvement in centrally induced calcitonin analgesia. Life Sci 22: 971-978PubMedCrossRefGoogle Scholar
  32. Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I (1985) Calcitonin gene- related peptide is a potent vasodilator. Nature 313:54-56PubMedCrossRefGoogle Scholar
  33. Broad PM, Symes AJ, Thakker RV, Craig RK (1989) Structure and méthylation of the human calcitonin/a-CGRP gene. Nucleic Acids Res 17:6999-7011PubMedCrossRefGoogle Scholar
  34. Bueno L, Fargeas MJ, Julie P (1986) Effects of calcitonin and CGRP alone or in combination on food intake and forestomach (reticulum) motility in sheep. Physiol Behav 36:907-911PubMedCrossRefGoogle Scholar
  35. Burns DM, Birnbaum RS, Roos BA (1989a) A neuroendocrine peptide derived from the amino-terminal half of rat procalcitonin. Mol Endocrinol 3:140-147PubMedCrossRefGoogle Scholar
  36. Burns DM, Forstrom JM, Friday KE, Howard GA, Roos BA (1989b) Procalci- tonin’s amino-terminal cleavage peptide is a bone-cell mitogen. Proc Natl Acad Sci USA 86:9519-9523PubMedCrossRefGoogle Scholar
  37. Cadieux A, Springall DR, Mulderry PK, Rodrigo J, Ghatei MA, Terenghi G, Bloom SR, Polak JM (1986) Occurrence, distribution and ontogeny of CGRP immunoreactivity in the rat lower respiratory tract: effect of capsaicin treatment and surgical denervations. Neuroscience 19:605-627PubMedCrossRefGoogle Scholar
  38. Cadieux A, Lanoue C, Sirois P, Barabé J (1990) Carbamylcholine- and 5-hydroxy- tryptamine-induced contraction in rat isolated airways: inhibition by calcitonin gene-related peptide. Br J Pharmacol 101:193-199PubMedGoogle Scholar
  39. Catherwood BD, Deftos LJ, Spiess J (1987) Complete sequence analysis of calci- tonin extracted from porcine pituitary. In: Cohn DV, Martin TJ, Meunier PJ (eds) Calcium regulation and bone metabolism. Basic and clinical aspects, vol 9. Excerpta Medica, Amsterdam, pp 82-87 (Proceedings of the 9th International Conference on Calcium Regulating Hormones and Bone Metabolism, 25 Oct–1 Nov, Nice 1986)Google Scholar
  40. Chabre O, Conklin BR, Lin HY, Lodish HF, Wilson E, Ives HE, Catanzariti L, Hemmings BA, Bourne HR (1992) A recombinant calcitonin receptor indepen- dently stimulates 3’,5’-cyclic adenosine monophosphate and Ca2+/insitol phosphate signaling pathways. Mol Endocrinol 6:551-556PubMedCrossRefGoogle Scholar
  41. Chakder S, Rattan S (1990) [Tyr0]-Calcitonin gene-related peptide 28-37 (rat) as a putative antagonist of calcitonin gene-related peptide responses on opossum internal anal sphincter smooth muscle. J Pharmacol Exp Ther 253:200-206PubMedGoogle Scholar
  42. Chambers TJ, Chambers JC, Symonds J, Darby JA (1986) The effect of human calcitonin on the cytoplasmic spreading of rat osteoclasts. J Clin Endocrinol Metab 63:1080-1085PubMedCrossRefGoogle Scholar
  43. Chiba T, Taminato T, Kadowaki S, Goto Y, Mori K, Seino Y, Abe H, Chihara K, Matsukura S, Fujita T, Kondo T (1980) Effects of [Asu1,7]-eel calcitonin on gastric somatostatin and gastrin release. Gut 21:94-97PubMedCrossRefGoogle Scholar
  44. Chiba T, Yamaguchi A, Yamatani T, Nakamura A, Morishita T, Inui T, Fukase M, Noda T, Fujita T (1989) Calcitonin gene-related peptide receptor antagonist human CGRP-(8-37). Am J Physiol 256:E331-E335PubMedGoogle Scholar
  45. Chihara K, Iwasaki J, Iwasaki Y, Minamitani N, Kaji H, Fujita T (1982) Central nervous system effect of calcitonin: stimulation of prolactin release in rats. Brain Res 248:331-339.PubMedCrossRefGoogle Scholar
  46. Clark MB, Williams CC, Nathanson BM, Horton RE, Glass HI, Foster GV (1974) Metabolic fate of human calcitonin in the dog. J Endocrinol 61:199-210PubMedCrossRefGoogle Scholar
  47. Clementi G, Nicoletti F, Patacchioli F, Prato A, Patti F, Fiore CE, Matera M, Scapagnini U (1983) Hypoprolactinemic action of calcitonin and the tubero- infundibular dopaminergic system. J Neurochem 40:885-886PubMedCrossRefGoogle Scholar
  48. Clementi G, Drago F, Prato A, Cavaliere S, di Benedetto A, Leone F, Scapagnini U, Rodolico G (1984) Effects of calcitonin, parathyroid hormone and its related fragments on acquisition of active avoidance behavior. Physiol Behav 33:913–916PubMedCrossRefGoogle Scholar
  49. Clementi G, Amico-Roxas M, Rapisarda E, Caruso A, Prato A, Trombadore S, Priolo G, Scapagnini U (1985) The analgesic activity of calcitonin and the central serotonergic system. Eur J Pharmacol 108:71-75PubMedCrossRefGoogle Scholar
  50. Conlon JM, Grimelius L, Thim L (1988a) Structural characterization of a high- molecular-mass form of calcitonin [procalcitonin-(60-116)-peptide] and its corresponding TV-terminal flanking peptide [procalcitonin-(l-57)-peptide] in a human medullary thyroid carcinoma. Biochem J 256:245-250PubMedGoogle Scholar
  51. Conlon JM, McGregor GP, Wallin G, Grimelius L, Thim L (1988b) Molecular forms of katacalcin, calcitonin gene-related peptide and gastrin-releasing peptide, in a human medullary thyroid carcinoma. Cancer Res 48:2412–2416PubMedGoogle Scholar
  52. Cooper CW, Schwesinger WH, Mahgoub AM, Ontjes DA (1971) Thyrocalcitonin: stimulation of secretion by pentagastrin. Science 172:1238-1240PubMedCrossRefGoogle Scholar
  53. Cooper GJS, Day AJ, Willis AC, Roberts AN, Reid KBM, Leighton B (1989) Amylin and the amylin gene: structure, function and relationship to islet amyloid and to diabetes mellitus. Biochim Biophys Acta 1014:247-258PubMedCrossRefGoogle Scholar
  54. Copp DH, Davidson AGF, Cheney B (1961) Evidence for a new parathyroid hormone which lowers blood calcium. Can Fed Biol Soc 4:17Google Scholar
  55. Copp DH, Cockroft DW, Kueh Y (1967) Calcitonin from ultimobranchial glands of dogfish and chickens. Science 158:924-925PubMedCrossRefGoogle Scholar
  56. Cortés R, Arvidsson U, Schalling M, Ceccatelli S, Hökfelt T (1990) In situ hybridiza- tion studies on mRNAs for cholecystokinin, calcitonin gene-related peptide and choline acetyltransferase in the lower brain stem, spinal cord and dorsal root ganglia of rat and guinea pig with special reference to motoneurons. J Chem Neuroanat 3:467-485PubMedGoogle Scholar
  57. Cote GJ, Gagel RF (1986) Dexamethasone differentially affects the levels of calcitonin and calcitonin gene-related peptide mRNAs expressed in a human medullary thyroid carcinoma cell line. J Biol Chem 261:15524-15528PubMedGoogle Scholar
  58. Cote GJ, Gould JA, Huang S-CE, Gagel RF (1987a) Studies of short-term secretion of peptides produced by alternative RNA processing. Mol Cell Endocrinol 53:211-219PubMedCrossRefGoogle Scholar
  59. Cote GJ, Rogers DG, Huang ESC, Gagel RF (1987b) The effect of 1,25-dihydroxy- vitamin D3 treatment on calcitonin and calcitonin gene-related peptide mRNA levels in cultured human thyroid C-cells. Biochem Biophys Res Commun 149: 239-243PubMedCrossRefGoogle Scholar
  60. Crenshaw EB III, Russo AF, Swanson LW, Rosenfeld MG (1987) Neuron-specific alternative RNA processing in transgenic mice expressing a métallothionein- calcitonin fusion gene. Cell 49:389-398PubMedCrossRefGoogle Scholar
  61. Crossman DC, Dashwood MR, Brain SD, McEwan J, Pearson JD (1990) Action of calcitonin gene-related peptide upon bovine vascular endothelial and smooth muscle cells grown in isolation and co-culture. Br J Pharmacol 99:71-76PubMedGoogle Scholar
  62. Dalsgaard C-J, Jernbeck J, Stains W, Kjartansson J, Hægerstrand A, Hökfelt T, Brodin E, Cuello AC, Brown JC (1989) Calcitonin gene-related peptide-like immunoreactivity in nerve fibers in the human skin. Histochemistry 91:35-38PubMedCrossRefGoogle Scholar
  63. Datta HK, Zaidi M, Wimalawansa SJ, Ghatei MA, Beacham JL, Bloom SR, MacIntyre I (1989) In vivo and in vitro effects of amylin and amylin-amide on calcium metabolism in the rat and rabbit. Biochem Biophys Res Commun 162:876-881PubMedCrossRefGoogle Scholar
  64. De Beaurepaire R, Suaudeau C (1988) Anorectic effect of calcitonin, neurotensin and bombesin infused in the area of the rostral part of the nucleus of the tractus solitarius in the rat. Peptides 9:729-733PubMedCrossRefGoogle Scholar
  65. De Bustros A, Baylin SB, Berger CL, Roos BA, Leong SS, Nelkin BD (1985) Phorbol esters increase calcitonin gene transcription and decrease c-myc mRNA levels in cultured human medullary thyroid carcinoma. J Biol Chem 260:98-104PubMedGoogle Scholar
  66. De Bustros A, Baylin SB, Levine MA, Nelkin BD (1986) Cyclic AMP and phorbol esters separately induce growth inhibition, calcitonin secretion, and calcitonin gene transcription in cultured human medullary thyroid carcinoma. J Biol Chem 261:8036-8041Google Scholar
  67. De Bustros A, Lee RY, Compton D, Tsong TY, Baylin SB, Nelkin BD (1990) Differential utilization of calcitonin gene regulatory DNA sequences in cultured lines of medullary thyroid carcinoma and small-cell lung carcinoma. Mol Cell Biol 10:1773-1778PubMedGoogle Scholar
  68. Deftos LJ, Roos BA (1989) Medullary thyroid carcinoma and calcitonin gene expression. Bone Miner Res 6:267-316Google Scholar
  69. Denis-Donini S (1989) Expression of dopaminergic phenotypes in the mouse olfactory bulb induced by the calcitonin gene-related peptide. Nature 339:701-703PubMedCrossRefGoogle Scholar
  70. Dennis T, Fournier A, St Pierre S, Quirion R (1989) Structure-activity profile of calcitonin gene-related peptide in peripheral and brain tissues. Evidence for receptor multiplicity. J Pharmacol Exp Ther 251:718-725PubMedGoogle Scholar
  71. Dennis T, Fournier A, Cadieux A, Pomerleau F, Jolicoeur FB, St Pierre S, Quirion R (1990) hCGRP8–37, a calcitonin gene-related peptide antagonist revealing calcitonin gene-related peptide receptor heterogeneity in brain and periphery. J Pharmacol Exp Ther 254:123-128PubMedGoogle Scholar
  72. Diez-Guerra FJ, Zaidi M, Bevis P, MacIntyre I, Emson PC (1988) Evidence for release of calcitonin gene-related peptide and neurokinin A from sensory nerve endings in vivo. Neuroscience 25:839-846PubMedCrossRefGoogle Scholar
  73. Dipette DJ, Schwarzenberger K, Kerr N, Holland OB (1989) Dose-dependent systemic and regional hemodynamic effects of calcitonin gene-related peptide. Am J Med Sci 297:65-70PubMedCrossRefGoogle Scholar
  74. Dotti-Sigrist S, Born W, Fischer JA (1988) Identification of a receptor for calcitonin gene-related peptides I and II in human cerebellum. Biochem Biophys Res Commun 151:1081-1087PubMedCrossRefGoogle Scholar
  75. Drack GT, Koelz HR, Blum AL (1976) Human calcitonin stimulates salivary amylase output in man. Gut 17:620-623PubMedCrossRefGoogle Scholar
  76. D’Souza SM, MacIntyre I, Girgis SI, Mundy GR (1986) Human synthetic calcitonin gene-related peptide inhibits bone resorption in vitro. Endocrinology 119:58-61PubMedCrossRefGoogle Scholar
  77. Dunning BE, Taborsky GJ Jr (1987) Calcitonin gene-related peptide: a potent and selective stimulator of gastrointestinal somatostatin secretion. Endocrinology 120:1774-1781PubMedCrossRefGoogle Scholar
  78. Edbrooke MR, Parker D, McVey JH, Riley JH, Sorenson GD, Pettengill OS, Craig RK (1985) Expression of the human calcitonin/CGRP gene in lung and thyroid carcinoma. EMBO J 4:715-724PubMedGoogle Scholar
  79. Edvinsson L, Fredholm BB, Hamel E, Jansen I, Verrecchia C (1985) Perivascular peptides relax cerebral arteries concomitant with stimulation of cyclic adenosine monophosphate accumulation or release of an endothelium-derived relaxing factor in the cat. Neurosci Lett 58:213-217PubMedCrossRefGoogle Scholar
  80. Edvinsson L, Ekman R, Jansen I, McCulloch J, Uddman R (1987) Calcitonin gene- related peptide and cerebral blood vessels: distribution and vasomotor effects. J Cereb Blood Flow Metab 7:720-728PubMedCrossRefGoogle Scholar
  81. Edwards RM, Trizna W (1990) Calcitonin gene-related peptide: effects on renal arteriolar tone and tubular cAMP levels. Am J Physiol 258:F121-F125PubMedGoogle Scholar
  82. Elie C, Moukhtar MS, Milhaud G, Cressent M (1990) Hypoprolactinemic effect of calcitonin gene-related peptide in the rat. Neuropeptides 16:109-113PubMedCrossRefGoogle Scholar
  83. Emeson RB, Hedjran F, Yeakley JM, Guise JW, Rosenfeld MG (1989) Alternative production of calcitonin and CGRP mRNA is regulated at the calcitonin-specific splice acceptor. Nature 341:76-80PubMedCrossRefGoogle Scholar
  84. Fahim A, Rettori V, McCann SM (1990) The role of calcitonin gene-related peptide in the control of growth hormone and prolactin release. Neuroendocrinology 51:688-693PubMedCrossRefGoogle Scholar
  85. Fargeas MJ, Fioramonti J, Buéno L (1984) Prostaglandin E2: a neuromodulator in the central control of gastrointestinal motility and feeding behavior by calcitonin. Science 225:1050-1052PubMedCrossRefGoogle Scholar
  86. Findlay DM, Michelangeli VP, Robinson PJ (1989) Protein kinase-C-induced down- regulation of calcitonin receptors and calcitonin-activated adenylate cyclase in T47D and BEN cells. Endocrinology 125:2656-2663PubMedCrossRefGoogle Scholar
  87. Fischer JA, Sagar SM, Martin JB (1981a) Characterization and regional distribution of calcitonin binding sites in the rat brain. Life Sci 29:663-671PubMedCrossRefGoogle Scholar
  88. Fischer JA, Tobler PH, Kaufmann M, Born W, Henke H, Cooper PE, Sagar SM, Martin JB (1981b) Calcitonin: regional distribution of the hormone and its binding sites in the human brain and pituitary. Proc Natl Acad Sci USA 78: 7801-7805PubMedCrossRefGoogle Scholar
  89. Fischer JA, Tobler PH, Henke H, Tschopp FA (1983) Salmon and human calcitonin- like peptides coexist in the human thyroid and brain. J Clin Endocrinol Metab 57:1314-1316PubMedCrossRefGoogle Scholar
  90. Fisher LA, Kikkawa DO, Rivier JE, Amara SG, Evans RM, Rosenfeld MG, Vale WW, Brown MR (1983) Stimulation of noradrenergic sympathetic outflow by calcitonin gene-related peptide. Nature 305:534-536PubMedCrossRefGoogle Scholar
  91. Fontaine B, Klarsfeld A, Hökfelt T, Changeux JP (1986) Calcitonin gene-related peptide, a peptide present in spinal cord motoneurons, increases the number of acetylcholine receptors in primary cultures of chick embryo myotubes. Neurosci Lett 71:59-65PubMedCrossRefGoogle Scholar
  92. Fontaine B, Klarsfeld A, Changeux JP (1987) Calcitonin gene-related peptide and muscle activity regulate acetylcholine receptor α-subunit mRNA levels by distinct intracellular pathways. J Cell Biol 105:1337-1342PubMedCrossRefGoogle Scholar
  93. Franco-Cereceda A, Gennari C, Nami R, Agnusdei D, Pernow J, Lundberg JM, Fischer JA (1987a) Cardiovascular effects of calcitonin gene-related peptides I and II in man. Circ Res 60:393-397PubMedGoogle Scholar
  94. Franco-Cereceda A, Henke H, Lundberg JM, Petermann JB, Hökfelt T, Fischer JA (1987b) Calcitonin gene-related peptide (CGRP) in capsaicin-sensitive substance P-immunoreactive sensory neurons in animals and man: distribution and release by capsaicin. Peptides 8:399-410PubMedCrossRefGoogle Scholar
  95. Franco-Cereceda A, Saria A, Lundberg JM (1989) Differential release of calcitonin gene-related peptide and neuropeptide Y from the isolated heart by capsaicin, ischaemia, nicotine, bradykinin and ouabain. Acta Physiol Scand 135:173–187PubMedCrossRefGoogle Scholar
  96. Freed WJ, Perlow MJ, Wyatt RJ (1979) Calcitonin: inhibitory effect on eating in rats. Science 206:850-852PubMedCrossRefGoogle Scholar
  97. Fried G, Franck J, Brodin E, Born W, Fischer JA, Hiort W, Hökfelt T (1989) Evidence for differential storage of calcitonin gene-related peptide, substance P and serotonin in synaptosomal vesicles of rat spinal cord. Brain Res 499:315–324PubMedCrossRefGoogle Scholar
  98. Gamse R, Saria A (1985) Potentiation of tachykinin-induced plasma protein extra- vasation by calcitonin gene-related peptide. Eur J Pharmacol 114:61-66PubMedCrossRefGoogle Scholar
  99. Gardiner SM, Compton AM, Bennett T (1989) Regional hemodynamic effects of calcitonin gene-related peptide. Am J Physiol 256:R332-R338PubMedGoogle Scholar
  100. Gardiner SM, Compton AM, Kemp PA, Bennett T, Bose C, Foulkes R, Hughes B (1990) Antagonistic effect of human α-CGRP [8-37] on the in vivo regional haemodynamic actions of human α-CGRP. Biochem Biophys Res Commun 171:938-943PubMedCrossRefGoogle Scholar
  101. Gennari C, Fischer JA (1985) Cardiovascular action of calcitonin gene-related peptide in humans. Calcif Tissue Int 37:581-584PubMedCrossRefGoogle Scholar
  102. Gennari C, Nami R, Agnusdei D, Fischer JA (1990) Improved cardiac performance with human calcitonin gene-related peptide in patients with congestive heart failure. Cardiovasc Res 24:239-241PubMedCrossRefGoogle Scholar
  103. Geppetti P, Maggi CA, Perretti F, Frilli S, Manzini S (1988) Simultaneous release by bradykinin of substance P- and calcitonin gene-related peptide immunoreactivities from capsaicin-sensitive structures in guinea-pig heart. Br J Pharmacol 94:288–290PubMedGoogle Scholar
  104. Geppetti P, Baldi E, Castellucci A, del Bianco E, Santicioli P, Maggi CA, Lippe IT, Amann R, Skofitsch G, Theodorsson E, Manzini S (1989) Calcitonin gene- related peptide in the rat kidney: occurrence, sensitivity to capsaicin, and stimulation of adenylate cyclase. Neuroscience 30:503–513PubMedCrossRefGoogle Scholar
  105. Geppetti P, Tramontana M, Santicioli P, del Bianco E, Giuliani S, Maggi CA (1990) Bradykinin-induced release of calcitonin gene-related peptide from capsaicin- sensitive nerves in guinea-pig atria: mechanism of action and calcium require- ments. Neuroscience 38:687-692PubMedCrossRefGoogle Scholar
  106. Gibbins IL, Furness JB, Costa M, MacIntyre I, Hillyard CJ, Girgis S (1985) Co- localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs. Neurosci Lett 57:125-130PubMedCrossRefGoogle Scholar
  107. Gibbins IL, Furness JB, Costa M (1987) Pathway-specific patterns of the co-existence of substance P, calcitonin gene-related peptide, cholecystokinin and dynorphin in neurons of the dorsal root ganglia of the guinea-pig. Cell Tissue Res 248:417–437PubMedCrossRefGoogle Scholar
  108. Gibson SJ, Polak JM, Bloom SR, Sabate IM, Mulderry PM, Ghatei MA, McGregor GP, Morrison JFB, Kelly JS, Evans RM, Rosenfeld MG (1984) Calcitonin gene- related peptide immunoreactivity in the spinal cord of man and of eight other species. J Neurosci 4:3101-3111PubMedGoogle Scholar
  109. Gibson SJ, Polak JM, Giaid A, Hamid QA, Kar S, Jones PM, Denny P, Legon S, Amara SG, Craig RK, Bloom SR, Penketh RJA, Rodek C, Ibrahim NBN, Dawson A (1988) Calcitonin gene-related peptide messenger RNA is expressed in sensory neurones of the dorsal root ganglia and also in spinal motoneurones in man and rat. Neurosci Lett 91:283-288PubMedCrossRefGoogle Scholar
  110. Gkonos PJ, Born W, Jones BN, Petermann JB, Keutmann HT, Birnbaum RS, Fischer JA, Roos BA (1986) Biosynthesis of calcitonin gene-related peptide and calcitonin by a human medullary thyroid carcinoma cell line. J Biol Chem 261:14386-14391PubMedGoogle Scholar
  111. Gnaedinger MP, Uehlinger DE, Weidmann P, Sha SG, Muff R, Born W, Rascher W, Fischer JA (1989) Distinct hemodynamic and renal effects of calcitonin gene- related peptide and calcitonin in men. Am J Physiol 257:E848-E854PubMedGoogle Scholar
  112. Goltzman D, Mitchell J (1985) Interaction of calcitonin and calcitonin gene-related peptide at receptor sites in target tissues. Science 227:1343-1345PubMedCrossRefGoogle Scholar
  113. Goto K, Kimura S, Saito A (1987) Inhibitory effect of calcitonin gene-related peptide on excitation and contraction of smooth muscles of the rat vas deferens. J Pharmacol Exp Ther 241:635-641PubMedGoogle Scholar
  114. Gray TK, Brannan P, Juan D, Morawski SG, Fordtran JS (1976) Ion transport changes during calcitonin-induced intestinal secretion in man. Gastroenterology 71:392-398PubMedGoogle Scholar
  115. Greenberg B, Rhoden K, Barnes P (1987) Calcitonin gene-related peptide (CGRP) is a potent non-endothelium-dependent inhibitor of coronary vasomotor tone. Br J Pharmacol 92:789-794PubMedGoogle Scholar
  116. Gropp C, Luster W, Havemann K (1985) Salmon and human calcitonin like material in lung cancer. Br J Cancer 51:897-901PubMedCrossRefGoogle Scholar
  117. Gruber HE, Ivey JL, Baylink DJ, Matthews M, Nelp WB, Sisom K, Chestnut CH III (1984) Long-term calcitonin therapy in postmenopausal osteoporosis. Metabolism 33:295-303PubMedCrossRefGoogle Scholar
  118. Guidobono F, Netti C, Pagani F, Sibilia V, Pecile A, Candeletti S, Ferri S (1986) Relationship of analgesia induced by centrally injected calcitonin to the CNS serotonergic system. Neuropeptides 8:259-271PubMedCrossRefGoogle Scholar
  119. Gulbenkian S, Merighi A, Wharton J, Varndell IM, Polak JM (1986) Ultrastructural evidence for the coexistence of calcitonin gene-related peptide and substance P in secretory vesicles of peripheral nerves in the guinea pig. J Neurocytol 15:535–542PubMedCrossRefGoogle Scholar
  120. Haas CA, Streit WJ, Kreutzberg GW (1990) Rat facial motoneurons express increased levels of calcitonin gene-related peptide mRNA in response to axotomy. J Neurosci Res 27:270-275PubMedCrossRefGoogle Scholar
  121. Haas HG, Dambacher MA, Guncaga J, Lauffenburger T (1971) Renal effects of calcitonin and parathyroid extract in man. J Clin Invest 50:2689-2702PubMedCrossRefGoogle Scholar
  122. Haddad JG, Caldwell JG (1972) Calcitonin resistance: clinical and immunologic studies in subjects with Paget’s disease of bone treated with porcine and salmon calcitonins. J Clin Invest 51:3133-3141PubMedCrossRefGoogle Scholar
  123. Haller-Brem S, Muff R, Fischer JA (1988) Calcitonin gene-related peptide and calcitonin secretion from a human medullary thyroid carcinoma cell line: effects of ionomycin, phorbol ester and forskolin. J Endocrinol 119:147-152PubMedCrossRefGoogle Scholar
  124. Ham J, Ellison ML, Lumsden J (1980) Tumour calcitonin. Interaction with specific calcitonin receptors. Biochem J 190:545-550PubMedGoogle Scholar
  125. Han S-P, Naes L, Westfall TC (1990) Inhibition of periarterial nerve stimulation- induced vasodilation of the mesenteric arterial bed by CGRP (8-37) and CGRP receptor desensitization. Biochem Biophys Res Commun 168:786-791PubMedCrossRefGoogle Scholar
  126. Henke H, Tobler PH, Fischer JA (1983) Localization of salmon calcitonin binding sites in rat brain by autoradiography. Brain Res 272:373-377PubMedCrossRefGoogle Scholar
  127. Henke H, Tschopp FA, Fischer JA (1985) Distinct binding sites for calcitonin gene- related peptide and salmon calcitonin in rat central nervous system. Brain Res 360:165-171PubMedCrossRefGoogle Scholar
  128. Henke H, Sigrist S, Lang W, Schneider J, Fischer JA (1987) Comparison of binding sites for the calcitonin gene-related peptides I and II in man. Brain Res 410: 404-408PubMedCrossRefGoogle Scholar
  129. Hill EL, Eide R (1988) Calcitonin gene-related peptide-immunoreactive nerve fibers in mandibular periosteum of rat: evidence for primary afferent origin. Neurosci Lett 85:172-178PubMedCrossRefGoogle Scholar
  130. Hirata Y, Takagi Y, Takata S, Fukuda Y, Yoshimi H, Fujita T (1988) Calcitonin gene-related peptide receptor in cultured vascular smooth muscle and endothelial cells. Biochem Biophys Res Commun 151:1113-1121PubMedCrossRefGoogle Scholar
  131. Holman JJ, Craig RK, Marshall I (1986) Human α- and β-CGRP and rat a-CGRP are coronary vasodilators in the rat. Peptides 7:231-235PubMedCrossRefGoogle Scholar
  132. Holzer P, Barthó L, Matusák O, Bauer V (1989) Calcitonin gene-related peptide action on intestinal circular muscle. Am J Physiol 256:G546-G552PubMedGoogle Scholar
  133. Homma T, Watanabe M, Hirose S, Kanai A, Kangawa K, Matsuo H (1986) Isolation and determination of the amino acid sequence of chicken calcitonin I from chicken ultimobranchial glands. J Biochem 100:459-467PubMedGoogle Scholar
  134. Höppener JWM, Steenbergh PH, Zandberg J, Bakker E, Pearson PL, Geurts van Kessel AHM, Jansz HS, Lips CJM (1984) Localization of the polymorphic human calcitonin gene on chromosome 11. Hum Genet 66:309-312PubMedCrossRefGoogle Scholar
  135. Höppener JWM, Steenbergh PH, Zandberg J, Geurts van Kessel AHM, Baylin SB, Nelkin BD, Jansz HS, Lips CJM (1985) The second human calcitonin/CGRP gene is located on chromosome 11. Hum Genet 70:259-263PubMedCrossRefGoogle Scholar
  136. Höppener JWM, Steenbergh PH, Moonen PJJ, Wagenaar SS, Jansz HS, Lips CJM (1986) Detection of mRNA encoding calcitonin, calcitonin gene-related peptide and proopiomelanocortin in human tumors. Mol Cell Endocrinol 47:125-130PubMedCrossRefGoogle Scholar
  137. Höppener JWM, Steenbergh PH, Slebos RJC, Visser A, Lips CJM, Jansz HS, Bechet JM, Lenoir GM, Born W, Haller-Brem S, Petermann JB, Fischer JA (1987) Expression of the second calcitonin/calcitonin gene-related peptide gene in Ewing sarcoma cell lines. J Clin Endocrinol Metab 64:809-817PubMedCrossRefGoogle Scholar
  138. Höppener JWM, Steenbergh PH, Zandberg J, Adema GJ, Geurts van Kessel AHM, Lips CJM, Jansz HS (1988) A third human CALC (pseudo) gene on chromosome 11. FEBS Lett 233:57-63PubMedCrossRefGoogle Scholar
  139. Horiuchi N, Takahashi H, Matsumoto T, Takahashi N, Shimazawa E, Suda T, Ogata E (1979) Salmon calcitonin-induced stimulation of lα,25-Dihydroxychol- ecalciferol synthesis in rats involving a mechanism independent of adenosine 3’:5’-cyclic monophosphate. Biochem J 184:269-275PubMedGoogle Scholar
  140. Hua XY, Theodorsson-Norheim E, Lundberg JM, Kinn A-C, Hökfelt T, Cuello AC (1987) Co-localization of tachykinins and calcitonin gene-related peptide in capsaicin-sensitive afferents in relation to motility effects on the human ureter in vitro. Neuroscience 23:693-703PubMedCrossRefGoogle Scholar
  141. Hughes JJ, Levine AS, Morley JE, Gosnell BA, Silvis SE (1984) Intraventricular calcitonin gene-related peptide inhibits gastric acid secretion. Peptides 5:665–667PubMedCrossRefGoogle Scholar
  142. Huwyler R, Born W, Ohnhaus EE, Fischer JA (1979) Plasma kinetics and urinary excretion of exogenous human and salmon calcitonin in man. Am J Physiol 236:E15-E19PubMedGoogle Scholar
  143. Inagaki S, Kito S, Kubota Y, Girgis S, Hillyard CJ, MacIntyre I (1986) Autoradio- graphic localization of calcitonin gene-related peptide binding sites in human and rat brains. Brain Res 374:287-298PubMedCrossRefGoogle Scholar
  144. Isaac R, Merceron R, Caillens G, Raymond J-P, Ardaillou R (1980) Effects of calcitonin on basal and thyrotropin-releasing hormone-stimulated prolactin secretion in man. J Clin Endocrinol Metab 50:1011-1015PubMedCrossRefGoogle Scholar
  145. Ishihara T, Nakamura S, Kaziro Y, Takahashi T, Takahashi K, Nagata S (1991) Molecular cloning and expression of a cDNA encoding the secretin receptor. EMBO J 10:1635-1641PubMedGoogle Scholar
  146. Ittner J, Dambacher MA, Born W, Ketelslegers JM, Buysschaert M, Albert PM, Lambert AE, Fischer JA (1985) Diagnostic evaluation of measurements of carboxyl-terminal flanking peptide (PDN-21) of the human calcitonin gene in human serum. J Clin Endocrinol Metab 61:1133–1137PubMedCrossRefGoogle Scholar
  147. Iwasaki Y, Chihara K, Iwasaki HA, Abe H, Fujita T (1979) Effect of calcitonin on prolactin release in rats. Life Sci 25:1243-1248PubMedCrossRefGoogle Scholar
  148. Jaeger P, Jones W, Clemens TL, Hayslett JP (1986) Evidence that calcitonin stim- ulates 1,25-dihydroxyvitamin D production and intestinal absorption of calcium in vivo. J Clin Invest 78:456-461PubMedCrossRefGoogle Scholar
  149. Jäger K, Muench R, Seifert H, Beglinger C, Bollinger A, Fischer JA (1990) Calcitonin gene-related peptide (CGRP) causes redistribution of blood flow in humans. Eur J Clin Pharmacol 39:491-494PubMedCrossRefGoogle Scholar
  150. Jennings CGB, Mudge AW (1989) Chick myotubes in culture express high-affinity receptors for calcitonin gene-related peptide. Brain Res 504:199-205PubMedCrossRefGoogle Scholar
  151. Johnston FG, Bell BA, Robertson IJA, Miller JD, Haliburn C, O’Shaughnessy D, Riddell AJ, O’Laoire SA (1990) Effect of calcitonin gene-related peptide on postoperative neurological deficits after subarachnoid haemorrhage. Lancet 335: 869-872PubMedCrossRefGoogle Scholar
  152. Jonas V, Lin CR, Kawashima E, Semon D, Swanson LW, Mermod J-J, Evans RM, Rosenfeld MG (1985) Alternative RNA processing events in human calcitonin/ calcitonin gene-related peptide gene expression. Proc Natl Acad Sci USA 82: 1994-1998PubMedCrossRefGoogle Scholar
  153. Ju G, Hökfelt T, Brodin E, Fahrenkrug J, Fischer JA, Frey P, Elde RP, Brown JC (1987) Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptide- and cholecystokinin-immunoreactive ganglion cells. Cell Tissue Res 247:417-431PubMedCrossRefGoogle Scholar
  154. Jüppner H, Abou-Samra A-B, Freeman M, Kong XF, Schipani E, Richards J, Kolakowski LF Jr, Hock J, Potts JT Jr, Kronenberg HM, Segre GV (1991) A G protein-linked receptor for parathyroid hormone and parathyroid hormone- related peptide. Science 254:1024-1026PubMedCrossRefGoogle Scholar
  155. Kahn SE, d’Alessio DA, Schwartz MW, Fujimoto WY, Ensinck JW, Taborsky GJ Jr, Porte D Jr (1990) Evidence of cosecretion of islet amyloid polypeptide and insulin by β-cells. Diabetes 39:634-638PubMedCrossRefGoogle Scholar
  156. Kanatsuka A, Makino H, Ohsawa H, Tokuyama Y, Yamaguchi T, Yoshida S, Adachi M (1989) Secretion of islet amyloid polypeptide in response to glucose. FEBS Lett 259:199-201PubMedCrossRefGoogle Scholar
  157. Kashihara Y, Sakaguchi M, Kuno M (1989) Axonal transport and distribution of endogenous calcitonin gene-related peptide in rat peripheral nerve. J Neurosci 9:3796-3802PubMedGoogle Scholar
  158. Katsoulis S, Conlon JM (1989) Calcitonin gene-related peptides relax guinea pig and rat gastric smooth muscle. Eur J Pharmacol 161:129-134CrossRefGoogle Scholar
  159. Kawai Y, Takami K, Shiosaka S, Emson PC, Hillyard CJ, Girgis S, MacIntyre I, Tohyama M (1985) Topographic localization of calcitonin gene-related peptide in the rat brain: an immunohistochemical analysis. Neuroscience 15:747–763PubMedCrossRefGoogle Scholar
  160. Kim S, Morimoto S, Kawai Y, Koh E, Onishi T, Ogihara T (1989) Circulating levels of calcitonin gene-related peptide in patients with medullary thyroid carcinoma. J Clin Chem Clin Biochem 27:423-427PubMedGoogle Scholar
  161. Kimura S, Sugita Y, Kanazawa I, Saito A, Goto K (1987) Isolation and amino acid sequence of calcitonin gene-related peptide from porcine spinal cord. Neuro- peptides 9:75-82Google Scholar
  162. Kline LW, Kaneko T, Chiu K-W, Harvey S, Pang PKT (1988) Calcitonin gene- related peptide in the bullfrog, Rana catesbeiana: localization and vascular actions. Gen Comp Endocrinol 72:123-129PubMedCrossRefGoogle Scholar
  163. Koop H, Eissele R, Kühlkamp V, Bothe E, Dionysius J, Arnold R (1987) Calcitonin gene-related peptide stimulates rat gastric somatostatin release in vitro. Life Sci 40:541-546PubMedCrossRefGoogle Scholar
  164. Kraenzlin ME, Ch’ng JLC, Mulderry PK, Ghatei MA, Bloom SR (1985) Infusion of a novel peptide, calcitonin gene-related peptide (CGRP) in man. Pharmacokin- etics and effects on gastric acid secretion and on gastrointestinal hormones. Regul Pept 10:189-197PubMedCrossRefGoogle Scholar
  165. Krahn DD, Gosnell BA, Levine AS, Morley JE (1984) Effects of calcitonin gene- related peptide on food intake. Peptides 5:861-864PubMedCrossRefGoogle Scholar
  166. Kreutter D, Orena SJ, Andrews KM (1989) Suppression of insulin-stimulated glucose transport in L6 myocytes by calcitonin gene-related peptide. Biochem Biophys Res Commun 164:461-467PubMedCrossRefGoogle Scholar
  167. Kruger L, Silverman JD, Mantyh PW, Sternini C, Brecha NC (1989) Peripheral patterns of calcitonin gene-related peptide general somatic sensory innervation: cutaneous and deep terminations. J Comp Neurol 280:291-302PubMedCrossRefGoogle Scholar
  168. Kubota M, Moseley JM, Butera L, Dusting GJ, MacDonald PS, Martin TJ (1985) Calcitonin gene-related peptide stimulates cyclic AMP formation in rat aortic smooth muscle cells. Biochem Biophys Res Commun 132:88-94PubMedCrossRefGoogle Scholar
  169. Kurtz A, Muff R, Born W, Lundberg JM, Millberg B-I, Gnädinger MP, Uehlinger DE, Weidmann P, Hökfelt T, Fischer JA (1988) Calcitonin gene-related peptide is a stimulator of renin secretion. J Clin Invest 82:538-543PubMedCrossRefGoogle Scholar
  170. Kurtz A, Schurek H-J, Jelkmann W, Muff R, Lipp H-P, Heckmann U, Eckardt K- U, Scholz H, Fischer JA, Bauer C (1989) Renal mesangium is a target for calcitonin gene-related peptide. Kidney Int 36:222-227PubMedCrossRefGoogle Scholar
  171. Lasmoles F, Jullienne A, Day F, Minvielle S, Milhaud G, Moukhtar MS (1985) Elucidation of the nucleotide sequence of chicken calcitonin mRNA: direct evidence for the expression of a lower vertebrate calcitonin-like gene in man and rat. EMBO J 4:2603-2607PubMedGoogle Scholar
  172. Laufer R, Changeux J-P (1989) Calcitonin gene-related peptide and cyclic AMP stimulate phosphoinositide turnover in skeletal muscle cells. J Biol Chem 264: 2683-2689PubMedGoogle Scholar
  173. Le Greves P, Nyberg F, Terenius L, Hökfelt T (1985) Calcitonin gene-related peptide is a potent inhibitor of substance P degradation. Eur J Pharmacol 115:309-311PubMedCrossRefGoogle Scholar
  174. Leighton B, Cooper GJS (1988) Pancreatic amylin and calcitonin gene-related peptide cause resistance to insulin in skeletal muscle in vitro. Nature 335:632-635PubMedCrossRefGoogle Scholar
  175. Lengyel A-MJ, Tannenbaum GS (1987) Mechanisms of calcitonin-induced growth hormone (GH) suppression: roles of somatostatin and GH-releasing factor. Endocrinology 120:1377-1383PubMedCrossRefGoogle Scholar
  176. Lenz HJ (1988) Calcitonin and CGRP inhibit gastrointestinal transit via distinct neuronal pathways. Am J Physiol 254.G920-G924Google Scholar
  177. Lenz HJ, Brown MR (1990) Cerebroventricular calcitonin gene-related peptide inhibits rat duodenal bicarbonate secretion by release of norepinephrine and vasopressin. J Clin Invest 85:25-32PubMedCrossRefGoogle Scholar
  178. Lenz HJ, Mortrud MT, Rivier JE, Brown MR (1985) Calcitonin gene-related peptide inhibits basal, pentagastrin, histamine, and bethanecol stimulated gastric acid secretion. Gut 26:550-555PubMedCrossRefGoogle Scholar
  179. Lenz HJ, Mortrud MT, Vale WW, Rivier E, Brown MR (1984) Calcitonin gene- related peptide acts within the central nervous system to inhibit gastric acid secretion. Regul Pept 9:271-277PubMedCrossRefGoogle Scholar
  180. Leung FW, Tallos EG, Taché YF, Guth PH (1987) Calcitonin gene-related peptide inhibits acid secretion without modifying blood flow. Am J Physiol 252:G215- G218PubMedGoogle Scholar
  181. Levy F, Muff R, Dotti-Sigrist S, Dambacher MA, Fischer JA (1988) Formation of neutralizing antibodies during intranasal synthetic salmon calcitonin treatment of Paget’s disease. J Clin Endocrinol Metab 67:541-545PubMedCrossRefGoogle Scholar
  182. Lindsay RM, Harmar AJ (1989) Nerve growth factor regulates expression of neuro- peptide genes in adult sensory neurons. Nature 337:362-364PubMedCrossRefGoogle Scholar
  183. Looij BJ Jr, Roelfsema F, van der Heide D, Frölich M, Souverijn JHM, Nieuwenhuizen Kruseman AC (1988) The effect of calcitonin on growth hormone secretion in man. Clin Endocrinol (Oxf) 29:517-527CrossRefGoogle Scholar
  184. Lundberg JM, Franco-Cereceda A, Hua X, Hökfelt T, Fischer JA (1985) Co- existence of substance P and calcitonin gene-related peptide-like immuno- reactivities in sensory nerves in relation to cardiovascular and bronchoconstrictor effects of capsaicin. Eur J Pharmacol 108:315-319PubMedCrossRefGoogle Scholar
  185. Lynch B, Kaiser ET (1988) Biological properties of two models of calcitonin gene- related peptide with idealized amphiphilic α-helices of different lengths. Bio- chemistry 27:7600-7607Google Scholar
  186. Macdonald NJ, Butters L, O’Shaughnessy DJ, Riddell AJ, Rubin PC (1989) A comparison of the effects of human alpha calcitonin gene-related peptide and glyceryl trinitrate on regional blood velocity in man. Br J Clin Pharmacol 28:257-261PubMedGoogle Scholar
  187. MacIntyre I, Hillyard CJ, Reynolds JJ, Gaines Das RE, Craig RK (1984) A second plasma calcium-lowering peptide from the human calcitonin precursor - a re- evaluation. Nature 308:84PubMedGoogle Scholar
  188. MacLean DB, Bennett B, Morris M, Wheeler FB (1989) Differential regulation of calcitonin gene-related peptide and substance P in cultured neonatal rat vagal sensory neurons. Brain Res 478:349-355PubMedCrossRefGoogle Scholar
  189. Maggi CA, Evangelista S, Giuliani S, Meli A (1987a) Anti-ulcer activity of calcitonin gene-related peptide in rats. Gen Pharmacol 18:33-34PubMedCrossRefGoogle Scholar
  190. Maggi CA, Giuliani S, Santicioli P, Abelli L, Meli A (1987b) Visceromotor responses to calcitonin gene-related peptide (CGRP) in the rat lower urinary tract: evidence for a transmitter role in the capsaicin-sensitive nerves of the ureter. Eur J Pharmacol 143:73-82PubMedCrossRefGoogle Scholar
  191. Maggi CA, Rovero P, Giuliani S, Evangelista S, Regoli D, Meli A (1990) Biological activity of N-terminal fragments of calcitonin gene-related peptide. Eur J Pharmacol 179:217-219PubMedCrossRefGoogle Scholar
  192. Malgaroli A, Meldolesi J, Zallone AZ, Teti A (1989) Control of cytosolic free calcium in rat and chicken osteoclasts. The role of extracellular calcium and calcitonin. J Biol Chem 264:14342-14347PubMedGoogle Scholar
  193. Manning MC (1989) Conformation of the alpha form of human calcitonin gene-related peptide (CGRP) in aqueous solution as determined by circular dichroism spec- troscopy. Biochem Biophys Res Commun 160:388-392PubMedCrossRefGoogle Scholar
  194. Marlier L, Rajaofetra N, Peretti-Renucci R, Kachidian P, Poulat P, Feuerstein C, Privat A (1990) Calcitonin gene-related peptide staining intensity is reduced in rat lumbar motoneurons after spinal cord transection: a quantitative immuno- cytochemical study. Exp Brain Res 82:40-47PubMedCrossRefGoogle Scholar
  195. Martial K, Minvielle S, Jullienne A, Segond N, Milhaud G, Lasmoles F (1990) Predicted structure of rabbit N-terminal, calcitonin and katacalcin peptides. Biochem Biophys Res Commun 171:1111-1114PubMedCrossRefGoogle Scholar
  196. Marx SJ, Woodard CJ, Aurbach GD (1972) Calcitonin receptors of kidney and bone. Science 178:999-1001PubMedCrossRefGoogle Scholar
  197. Mason RT, Peterfreund RA, Sawchenko PE, Corrigan AZ, Rivier JE, Vale WW (1984) Release of predicted calcitonin gene-related peptide from cultured rat trigeminal ganglion cells. Nature 308:653-655PubMedCrossRefGoogle Scholar
  198. Mason RT, Shulkes A, Zajac JD, Fletcher AE, Hardy KJ, Martin TJ (1986) Basal and stimulated release of calcitonin gene-related peptide (CGRP) in patients with medullary thyroid carcinoma. Clin Endocrinol (Oxf) 25:675-685CrossRefGoogle Scholar
  199. Maton PN, Pradhan T, Zhou Z-C, Gardner JD, Jensen RT (1990) Activities of calcitonin gene-related peptide (CGRP) and related peptides at the CGRP receptor. Peptides 11:485-489PubMedCrossRefGoogle Scholar
  200. Mazzuoli GF, Tabolli S, Bigi F, Valtorta C, Minisola S, Diacinti D, Scarnecchia L, Bianchi G, Piolini M, delۥAcqua S (1990) Effects of salmon calcitonin on the bone loss induced by ovariectomy. Calcif Tissue Int 47:209-214PubMedCrossRefGoogle Scholar
  201. McCormack DG, Mak JCW, Coupe MO, Barnes PJ (1989) Calcitonin gene-related peptide vasodilation of human pulmonary vessels. J Appl Physiol 67:1265–1270PubMedGoogle Scholar
  202. McCulloch CR, Cooke HJ (1989) Human α-calcitonin gene-related peptide influences colonic secretion by acting on myenteric neurons. Regul Pept 24:87-96PubMedCrossRefGoogle Scholar
  203. McLean GW, Rabin D, Moore L, Deftos LJ, Lorber D, McKenna TJ (1984) Evaluation of provocative tests in suspected medullary carcinoma of the thyroid: heterogeneity of calcitonin responses to calcium and pentagastrin. Metabolism 33:790-796PubMedCrossRefGoogle Scholar
  204. Merighi A, Polak JM, Gibson SJ, Gulbenkian S, Valentino KL, Peirone SM (1988) Ultrastructural studies on calcitonin gene-related peptide-, tachykinins- and somatostatin-immunoreactive neurones in rat dorsal root ganglia: evidence for the colocalization of different peptides in single secretory granules. Cell Tissue Res 254:101-109PubMedCrossRefGoogle Scholar
  205. Michelangeli VP, Findlay DM, Fletcher A, Martin TJ (1986) Calcitonin gene-related peptide (CGRP) acts independently of calcitonin on cyclic AMP formation in clonal osteogenic sarcoma cells (UMR 106-01). Calcif Tissue Int 39:44-48PubMedCrossRefGoogle Scholar
  206. Michelangeli VP, Fletcher AE, Allan EH, Nicholson GC, Martin TJ (1989) Effects of calcitonin gene-related peptide on cyclic AMP formation in chicken, rat, and mouse bone cells. J Bone Miner Res 4:269-272PubMedCrossRefGoogle Scholar
  207. Miles K, Greengard P, Huganir RL (1989) Calcitonin gene-related peptide regulates phosphorylation of the nicotinic acetylcholine receptor in rat myotubes. Neuron 2:1517-1524PubMedCrossRefGoogle Scholar
  208. Minvielle S, Cressent M, Lasmoles F, Jullienne A, Milhaud G, Moukhtar MS (1986) Isolation and partial characterization of the calcitonin gene in a lower vertebrate. FEBS Lett 203:7-10PubMedCrossRefGoogle Scholar
  209. Minvielle S, Cressent M, Delehaye MC, Segond N, Milhaud G, Jullienne A, Moukhtar MS, Lasmoles F (1987) Sequence and expression of the chicken calcitonin gene. FEBS Lett 223:63-68PubMedCrossRefGoogle Scholar
  210. Mitsuma T, Nogimori T, Chaya M (1984) Peripheral administration of eel calcitonin inhibits thyrotropin secretion in rats. Eur J Pharmacol 102:123-128PubMedCrossRefGoogle Scholar
  211. Miyauchi T, Sano Y, Hiroshima O, Yuzuriha T, Sugishita Y, Ishikawa T, Saito A, Goto K (1988) Positive inotropic effects and receptors of calcitonin gene-related peptide (CGRP) in porcine ventricular muscles. Biochem Biophys Res Commun 155:289-294PubMedCrossRefGoogle Scholar
  212. Molina JM, Cooper GJS, Leighton B, Olefsky JM (1990) Induction of insulin resistance in vivo by amylin and calcitonin gene-related peptide. Diabetes 39: 260-265PubMedCrossRefGoogle Scholar
  213. Mora M, Marchi M, Polak JM, Gibson SJ, Cornelio F (1989) Calcitonin gene-related peptide immunoreactivity at the human neuromuscular junction. Brain Res 492:404-407PubMedCrossRefGoogle Scholar
  214. Morel F (1983) Regulation of kidney functions by hormones: a new approach. Recent Prog Horm Res 39:271-304PubMedGoogle Scholar
  215. Morimoto T, Okamoto M, Koida M, Nakamuta H, Stahl GL, Orlowski RC (1985) Intracerebroventricular injection of 125I-salmon calcitonin in rats: fate, anorexia and hypocalcemia. Jpn J Pharmacol 37:21-29PubMedCrossRefGoogle Scholar
  216. Morley JE, Levine AS, Silvis SE (1981) Intraventricular calcitonin inhibits gastric acid secretion. Science 214:671-673PubMedCrossRefGoogle Scholar
  217. Morris HR, Panico M, Etienne T, Tippins J, Girgis SI, MacIntyre I (1984) Isolation and characterization of human calcitonin gene-related peptide. Nature 308: 746-748PubMedCrossRefGoogle Scholar
  218. Morton CR, Hutchison WD (1989) Release of sensory neuropeptides in the spinal cord: studies with calcitonin gene-related peptide and galanin. Neuroscience 31:807-815PubMedCrossRefGoogle Scholar
  219. Moseley JM, Smith P, Martin TJ (1986) Identification of the calcitonin receptor by chemical cross-linking and photoaffinity labeling in human cancer cell lines. J Bone Miner Res 1:293-297PubMedCrossRefGoogle Scholar
  220. Mosselman S, Höppener JWM, Zandberg J, van Mansfeld ADM, Geurts van Kessel AHM, Lips CJM, Jansz HS (1988) Islet amyloid polypeptide: identification and chromosomal localization of the human gene. FEBS Lett 239:227-232PubMedCrossRefGoogle Scholar
  221. Muff R, Dambacher MA, Perrenoud A, Simon C, Fischer JA (1990) Efficacy of intranasal human calcitonin in patients with Paget’s disease refractory to salmon calcitonin. Am J Med 89:181-184PubMedCrossRefGoogle Scholar
  222. Muff R, Dambacher MA, Fischer JA (1991) Formation of neutralizing antibodies during intranasal synthetic salmon calcitonin treatment of postmenopausal osteoporosis. Osteoporosis Int 1:72-75CrossRefGoogle Scholar
  223. Mulderry PK, Ghatei MA, Rodrigo J, Allen JM, Rosenfeld MG, Polak JM, Bloom SR (1985) Calcitonin gene-related peptide in cardiovascular tissues of the rat. Neuroscience 14:947-954PubMedCrossRefGoogle Scholar
  224. Mulderry PK, Ghatei MA, Spokes RA, Jones PM, Pierson AM, Hamid QA, Kanse S, Amara SG, Burrin JM, Legon S, Polak JM, Bloom SR (1988) Differential expression of α-CGRP and β-CGRP by primary sensory neurons and enteric autonomic neurons of the rat. Neuroscience 25:195-205PubMedCrossRefGoogle Scholar
  225. Mulholland MW, Jaffer S (1990) Stimulation of acetylcholine release in myenteric plexus by calcitonin gene-related peptide. Am J Physiol 259:G934-G939PubMedGoogle Scholar
  226. Murphy E, Chamberlin ME, Mandel LJ (1986) Effects of calcitonin on cytosolic Ca in a suspension of rabbit medullary thick ascending limb tubules. Am J Physiol 251:C491-C495PubMedGoogle Scholar
  227. Murphy S, Osborne PB, Adamson S, Campbell G (1990) Co-localization of calcitonin gene-related peptide- and substance P-like immunoreactivity in mucosal intra- epithelial nerves in the toad colon. Neurosci Lett 116:7-11PubMedCrossRefGoogle Scholar
  228. Naveh-Many T, Silver J (1988) Regulation of calcitonin gene transcription by vitamin D metabolites in vivo in the rat. J Clin Invest 81:270-273PubMedCrossRefGoogle Scholar
  229. Nealon WH, Beauchamp RD, Townsend CM, Thompson JC (1990) Additive interactions of calcitonin gene-related peptide and calcitonin on pancreatic exocrine function in conscious dogs. Surgery 107:434-441PubMedGoogle Scholar
  230. Nelkin BD, Rosenfeld KI, de Bustros A, Leong SS, Roos BA, Baylin SB (1984) Structure and expression of a gene encoding human calcitonin and calcitonin gene-related peptide. Biochem Biophys Res Commun 123:648-655PubMedCrossRefGoogle Scholar
  231. Nelkin BD, Chen KY, de Bustros A, Roos BA, Baylin SB (1989) Changes in calcitonin gene RNA processing during growth of a human medullary thyroid carcinoma cell line. Cancer Res 49:6949-6952PubMedGoogle Scholar
  232. Nelson MT, Huang Y, Brayden JE, Hescheler J, Standen NB (1990) Arterial dilations in response to calcitonin gene-related peptide involve activation of K+ channels. Nature 344:770-773PubMedCrossRefGoogle Scholar
  233. New HV, Mudge AW (1986) Calcitonin gene-related peptide regulates muscle acetylcholine receptor synthesis. Nature 323:809-811PubMedCrossRefGoogle Scholar
  234. Nicholson GC, Moseley JM, Sexton PM, Mendelsohn FAO, Martin TJ (1986) Abundant calcitonin receptors in isolated rat osteoclasts. J Clin Invest 78:355–360PubMedCrossRefGoogle Scholar
  235. Nicholson GC, Moseley JM, Yates AJP, Martin TJ (1987) Control of cyclic adenosine 3’,5’-monophosphate production in osteoclasts: calcitonin-induced persistent activation and homologous desensitization of adenylate cyclase. Endocrinology 120:1902-1908PubMedCrossRefGoogle Scholar
  236. Nicholson GC, D’Santos CS, Evans T, Moseley JM, Kemp BE, Michelangeli VP, Martin TJ (1988) Human placental calcitonin receptors. Biochem J 250:877-882PubMedGoogle Scholar
  237. Nicoletti F, Clementi G, Patti F, Canonico PL, di Giorgio RM, Matera M, Pennisi G, Angelucci L, Scapagnini U (1982) Effects of calcitonin on rat extrapyramidal motor system: behavioral and biochemical data. Brain Res 250:381-385PubMedCrossRefGoogle Scholar
  238. Nilsson G, Alving K, Ahlstedt S, Hökfelt T, Lundberg JM (1990) Peptidergic innervation of rat lymphoid tissue and lung: relation to mast cells and sensitivity to capsaicin and immunization. Cell Tissue Res 262:125-133PubMedCrossRefGoogle Scholar
  239. Nishi M, Sanke T, Seino S, Eddy RL, Fan Y-S, Byers MG, Shows TB, Bell GI, Steiner DF (1989) Human islet amyloid polypeptide gene: complete nucleotide sequence, chromosomal localization, and evolutionary history. Mol Endocrinol 3:1775-1781PubMedCrossRefGoogle Scholar
  240. Nishizawa Y, Okui Y, Inaba M, Okuno S, Yukioka K, Miki T, Watanabe Y, Morii H (1988) Calcium/calmodulin-mediated action of calcitonin on lipid metabolism in rats. J Clin Invest 82:1165-1172PubMedCrossRefGoogle Scholar
  241. Noel M, Gavoille A, Lasmoles F, Kahn E, Caillou B, Gardet P, Fragu P (1990) Quantification of intracellular calcitonin gene transcripts in human medullary thyroid carcinoma (MTC) by in situ hybridization. J Endocrinol Invest 13:567–573PubMedGoogle Scholar
  242. Noguchi K, Senba E, Morita Y, Sato M, Tohyama M (1990a) a-CGRP and β-CGRP mRNAs are differentially regulated in the rat spinal cord and dorsal root ganglion. Mol Brain Res 7:299-304PubMedCrossRefGoogle Scholar
  243. Noguchi K, Senba E, Morita Y, Sato M, Tohyama M (1990b) Co-expression of α- CGRP and β-CGRP mRNAs in the rat dorsal root ganglion cells. Neurosci Lett 108:1-5PubMedCrossRefGoogle Scholar
  244. O’Brien TD, Westermark P, Johnson KH (1990) Islet amyloid polypeptide (IAPP) does not inhibit glucose-stimulated insulin secretion from isolated perfused rat pancreas. Biochem Biophys Res Commun 170:1223-1228PubMedCrossRefGoogle Scholar
  245. Ohhashi T, Jacobowitz DM (1988) Effects of calcitonin gene-related peptide on neuromuscular transmission in the isolated rat diaphragm. Peptides 9:613-617PubMedCrossRefGoogle Scholar
  246. Ohmura T, Nishio M, Kigoshi S, Muramatsu I (1990) Electrophysiological and mechanical effects of calcitonin gene-related peptide on guinea-pig atria. Br J Pharmacol 100:27-30PubMedGoogle Scholar
  247. Ohsawa H, Kanatsuka A, Yamaguchi T, Makino H, Yoshida S (1989) Islet amyloid polypeptide inhibits glucose-stimulated insulin secretion from isolated rat pancreatic islets. Biochem Biophys Res Commun 160:961-967PubMedCrossRefGoogle Scholar
  248. Ohtani R, Kaneko T, Kline LW, Labedz T, Tang Y, Pang PKT (1989) Localization of calcitonin gene-related peptide in the small intestine of various vertebrate species. Cell Tissue Res 258:35-42PubMedCrossRefGoogle Scholar
  249. Olgiati VR, Guidobono F, Netti C, Pecile A (1983) Localization of calcitonin binding sites in rat central nervous system: evidence of its neuroactivity. Brain Res 265:209-215PubMedCrossRefGoogle Scholar
  250. Ono K, Delay M, Nakajima T, Irisawa H, Giles W (1989) Calcitonin gene-related peptide regulates calcium current in heart muscle. Nature 340:721-724PubMedCrossRefGoogle Scholar
  251. Palmer JBD, Cuss FMC, Mulderry PK, Ghatei MA, Springall DR, Cadieux A, Bloom SR, Polak JM, Barnes PJ (1987) Calcitonin gene-related peptide is localised to human airway nerves and potently constricts human airway smooth muscle. Br J Pharmacol 91:95-101PubMedGoogle Scholar
  252. Pappas T, Debas HT, Walsh JH, Rivier J, Taché Y (1986) Calcitonin gene-related peptide-induced selective inhibition of gastric acid secretion in dogs. Am J Physiol 250:G127-G133PubMedGoogle Scholar
  253. Pecile A, Ferri S, Braga PC, Olgiati VR (1975) Effects of intracerebroventricular calcitonin in the conscious rabbit. Experientia 31:332-333PubMedCrossRefGoogle Scholar
  254. Pecile A, Guidobono F, Netti C, Sibilia V, Biella G, Braga PC (1987) Calcitonin gene-related peptide: antinociceptive activity in rats, comparison with calcitonin. Regul Pept 18:189-199PubMedCrossRefGoogle Scholar
  255. Peleg S, Abruzzese RV, Cote GJ, Gagel RF (1990) Transcription of the human calcitonin gene is mediated by a C cell-specific enhancer containing E-box-like elements. Mol Endocrinol 4:1750-1757PubMedCrossRefGoogle Scholar
  256. Pennefather JN, Reynoldson NA, Handberg GM (1990) Inhibition of rat uterine contractions by rat and human CGRP. Peptides 11:903-906PubMedCrossRefGoogle Scholar
  257. Perez Cano R, Girgis SI, Galan Galan F, MacIntyre I (1982a) Identification of both human and salmon calcitonin-like molecules in birds suggesting the existence of two calcitonin genes. J Endocrinol 92:351-355PubMedCrossRefGoogle Scholar
  258. Perez Cano R, Girgis SI, MacIntyre I (1982b) Further evidence for calcitonin gene duplication: the identification of two different calcitonins in a fish, a reptile and two mammals. Acta Endocrinol (Copenh) 100:256-261Google Scholar
  259. Peterfreund RA, Vale WW (1986) Local anesthetics inhibit veratridine-induced secretion of calcitonin gene-related peptide (CGRP) from cultured rat trigeminal ganglion cells. Brain Res 380:159-161PubMedCrossRefGoogle Scholar
  260. Petermann JB, Born W, Chang JY, Fischer JA (1987) Identification in the human central nervous system, pituitary, and thyroid of a novel calcitonin gene-related peptide, and partial amino acid sequence in the spinal cord. J Biol Chem 262:542-545PubMedGoogle Scholar
  261. Pettersson M, Ahrén B (1988) Insulin and glucagon secretion in rats: effects of calcitonin gene-related peptide. Regul Pept 23:37-50PubMedCrossRefGoogle Scholar
  262. Pontiroli AE (1985) Intranasal calcitonin and plasma calcium concentrations. Br Med J 291: 54-55CrossRefGoogle Scholar
  263. Popper P, Micevych PE (1990) Steroid regulation of calcitonin gene-related peptide mRNA expression in motoneurons of the spinal nucleus of the bulbocavernosus. Mol Brain Res 8:159-166PubMedCrossRefGoogle Scholar
  264. Pöschl E, Lindley I, Hofer E, Seifert J-M, Brunowsky W, Besemer J (1987) The structure of procalcitonin of the salmon as deduced from its cDNA sequence. FEBS Lett 226:96-100PubMedCrossRefGoogle Scholar
  265. Primi MP, Bueno L (1986) Centrally mediated stimulation of jejunal water and electrolyte secretion by calcitonin in dogs. Am J Physiol 250:G172-G176PubMedGoogle Scholar
  266. Rattan S, Gonnella P, Goyal RK (1988) Inhibitory effect of calcitonin gene-related peptide and calcitonin on opossum esophageal smooth muscle. Gastroenterology 94:284-293PubMedGoogle Scholar
  267. Reginster JY, Albert A, Lecart MP, Labelin P, Denis D, Deroisy R, Fontaine MA, Franchimont P (1987) 1-year controlled randomised trial of prevention of early postmenopausal bone loss by intranasal calcitonin. Lancet 2:1481-1483PubMedCrossRefGoogle Scholar
  268. Reinecke M, Forssmann WG (1988) Neuropeptide (neuropeptide Y, neurotensin, vasoactive intestinal polypeptide, substance P, calcitonin gene-related peptide, somatostatin) immunohistochemistry and ultrastructure of renal nerves. Histo- chemistry 89:1-9Google Scholar
  269. Réthelyi M, Metz CB, Lund PK (1989) Distribution of neurons expressing calcitonin gene-related peptide mRNAs in the brain stem, spinal cord and dorsal root ganglia of rat and guinea-pig. Neuroscience 29:225-239PubMedCrossRefGoogle Scholar
  270. Riley JH, Edbrooke MR, Craig RK (1986) Ectopic synthesis of high-Mr calcitonin by the BEN lung carcinoma cell line reflects aberrant proteolytic processing. FEBS Lett 198:71-79PubMedCrossRefGoogle Scholar
  271. Roos BA, Fischer JA, Pignat W, Alander CB, Raisz LG (1986) Evaluation of the in vivo and in vitro calcium-regulating actions of noncalcitonin peptides produced via calcitonin gene expression. Endocrinology 118:46-51PubMedCrossRefGoogle Scholar
  272. Rosenfeld MG, Mermod J-J, Amara SG, Swanson LW, Sawchenko PE, Rivier J, Vale WW, Evans RM (1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature 304:129-135PubMedCrossRefGoogle Scholar
  273. Russo AF, Nelson C, Roos BA, Rosenfeld MG (1988) Differential regulation of the coexpressed calcitonin/α-CGRP and β-CGRP neuroendocrine genes. J Biol Chem 263:5-8PubMedGoogle Scholar
  274. Sabate MI, Stolarsky LS, Polak JM, Bloom SR, Varndell IM, Ghatei MA, Evans RM, Rosenfeld MG (1985) Regulation of neuroendocrine gene expression by alternative RNA processing. J Biol Chem 260:2589-2592PubMedGoogle Scholar
  275. Sabbatini F, Fimmel CJ, Pace F, Tobler PH, Hinder RA, Blum AL, Fischer JA (1985) Distribution of intraventricular salmon calcitonin and suppression of gastric secretion. Digestion 32:273-281PubMedCrossRefGoogle Scholar
  276. Saito A, Masaki T; Uchiyama Y, Lee TJ-F, Goto K (1989) Calcitonin gene-related peptide and vasodilator nerves in large cerebral arteries of cats. J Pharmacol Exp Ther 248:455-462PubMedGoogle Scholar
  277. Samuelson UE, Dalsgaard C-J, Lundberg JM, Hökfelt T (1985) Calcitonin gene- related peptide inhibits spontaneous contractions in human uterus and fallopian tube. Neurosci Lett 62:225-230PubMedCrossRefGoogle Scholar
  278. Sano Y, Hiroshima O, Yuzuriha T, Yamato C, Saito A, Kimura S, Hirabayashi T, Goto K (1989) Calcitonin gene-related peptide-binding sites of porcine cardiac muscles and coronary arteries: solubilization and characterization. J Neurochem 52:1919-1924PubMedCrossRefGoogle Scholar
  279. Saria A, Gamse R, Petermann J, Fischer JA, Theodorsson-Norheim E, Lundberg J- M (1986) Simultaneous release of several tachykinins and calcitonin gene-related peptide from rat spinal cord slices. Neurosci Lett 63:310-314PubMedCrossRefGoogle Scholar
  280. Sasayama Y, Matsuda K, Oguro C, Kambegawa A (1989) Immunohistochemical demonstration of calcitonin gene-related peptide in the ultimobranchial gland of some lower vertebrates and in the nervous tissues of some invertebrates. Zool Sci 6:423-426Google Scholar
  281. Scherübl H, Schultz G, Hescheler J (1990) A slowly inactivating calcium current works as a calcium sensor in calcitonin-secreting cells. FEBS Lett 273:51-54PubMedCrossRefGoogle Scholar
  282. Schiebinger RJ, Santora AC (1989) Stimulation by calcitonin gene-related peptide of atrial natriuretic peptide secretion in vitro and its mechanism of action. Endo- crinology 124:2473-2479Google Scholar
  283. Schifter S, Johannsen L, Aagaard MT, Goltermann N, Parkes HC, Craig RK (1989) Elevated serum levels of calcitonin gene-related peptide (CGRP) but no evidence for CGRP gene expression in non-small cell lung carcinomas. Clin Endocrinol (Oxf) 31:137-142CrossRefGoogle Scholar
  284. Scott Helton W, Mulholland MM, Bunnett NW, Debas HT (1989) Inhibition of gastric and pancreatic secretion in dogs by CGRP: role of somatostatin. Am J Physiol 256:G715-G720Google Scholar
  285. Segond N, Legendre B, Tahri EH, Besnard P, Jullienne A, Moukhtar MS, Garel J- M (1985) Increased level of calcitonin mRNA after 1,25-dihydroxyvitamin D3 injection in the rat. FEBS Lett 184:268-272PubMedCrossRefGoogle Scholar
  286. Segond N, Delehaye MC, Jullienne A, Taboulet J, Minvielle S, Milhaud G, Moukhtar MS (1989) Actinomycin D inhibits the rapid increase in translatable calcitonin mRNA provoked by acute calcium stimulation. Horm Metab Res 21:489-493PubMedCrossRefGoogle Scholar
  287. Seifert H, Chesnut J, de Souza E, Rivier J, Vale W (1985) Binding sites for calcitonin gene-related peptide in distinct areas of rat brain. Brain Res 346:195–198PubMedCrossRefGoogle Scholar
  288. Seitz PK, Cooper CW (1989) Cosecretion of calcitonin and calcitonin gene-related peptide from cultured rat medullary thyroid C cells. J Bone Miner Res 4:129–134PubMedCrossRefGoogle Scholar
  289. Sexton PM, McKenzie JS, Mason RT, Moseley JM, Martin TJ, Mendelsohn FAO (1986) Localization of binding sites for calcitonin gene-related peptide in rat brain by in vitro autoradiography. Neuroscience 19:1235-1245PubMedCrossRefGoogle Scholar
  290. Sexton PM, Adam WR, Moseley JM, Martin TJ, Mendelsohn FAO (1987) Localiza- tion and characterization of renal calcitonin receptors by in vitro autoradiography. Kidney Int 32:862-868PubMedCrossRefGoogle Scholar
  291. Sexton PM, McKenzie JS, Mendelsohn FAO (1988) Evidence for a new subclass of calcitonin/calcitonin gene-related peptide binding site in rat brain. Neurochem Int 12:323-335PubMedCrossRefGoogle Scholar
  292. Shah GV, Wang W, Grosvenor CE, Crowley WR (1990) Calcitonin inhibits basal and thyrotropin-releasing hormone-induced release of prolactin from anterior pituitary cells: evidence for a selective action exerted proximal to secretagogue- induced increases in cytosolic Ca2+. Endocrinology 127:621-628PubMedCrossRefGoogle Scholar
  293. Shimizu N, Oomura Y (1986) Calcitonin-induced anorexia in rats: evidence for its inhibitory action on lateral hypothalamic chemosensitive neurons. Brain Res 367:128-140PubMedCrossRefGoogle Scholar
  294. Sigrist S, Franco-Cereceda A, Muff R, Henke H, Lundberg JM, Fischer JA (1986) Specific receptor and cardiovascular effects of calcitonin gene-related peptide. Endocrinology 119:381-389PubMedCrossRefGoogle Scholar
  295. Simmons RE, Hjelle JT, Mahoney C, Deftos LJ, Lisker W, Kato P, Rabkin R (1988) Renal metabolism of calcitonin. Am J Physiol 254:F593-F600PubMedGoogle Scholar
  296. Singer FR, Mills BG (1984) Paget’s disease of bone: etiologic and therapeutic aspects. Bone Miner Res 2:394-421Google Scholar
  297. Singer FR, Habener JF, Greene E, Godin P, Potts JT Jr (1972a) Inactivation of calcitonin by specific organs. Nature [New Biol] 237:269-270CrossRefGoogle Scholar
  298. Singer FR, Aldred JP, Neer RM, Krane SM, Potts JT Jr, Bloch KJ (1972b) An evaluation of antibodies and clinical resistance to salmon calcitonin. J Clin Invest 51:2331-2338PubMedCrossRefGoogle Scholar
  299. Skofitsch G, Jacobowitz DM (1985) Autoradiographic distribution of 125I calcitonin gene-related peptide binding sites in the rat central nervous system. Peptides 4:975-986CrossRefGoogle Scholar
  300. Stangl D, Born W, Fischer JA (1991) Characterization and photoaffinity labeling of a calcitonin gene-related peptide receptor solubilized from human cerebellum. Biochemistry 30:8605-8611PubMedCrossRefGoogle Scholar
  301. Steenbergh PH, Höppener JWM, Zandberg J, van de Ven WJM, Jansz HS, Lips CJM (1984) Calcitonin gene-related peptide coding sequence is conserved in the human genome and is expressed in medullary thyroid carcinoma. J Clin Endo- crinol Metab 59:358-360CrossRefGoogle Scholar
  302. Steenbergh PH, Höppener JWM, Zandberg J, Cremers AFM, Jansz HS, Lips CJM (1985a) Structure of the human calcitonin gene and its transcripts in medullary thyroid carcinoma. In: Pecile A (ed) Calcitonin. Elsevier Amsterdam, pp 23–31Google Scholar
  303. Steenbergh PH, Höppener JWM, Zandberg J, Lips CJM, Jansz HS (1985b) A second human calcitonin/CGRP gene. FEBS Lett 183:403-407PubMedCrossRefGoogle Scholar
  304. Steenbergh PH, Höppener JWM, Zandberg J, Visser A, Lips CJM, Jansz HS (1986) Structure and expression of the human calcitonin/CGRP genes. FEBS Lett 209:97-103PubMedCrossRefGoogle Scholar
  305. Stevenson JC, Adrian TE, Christofides ND, Bloom SR (1985) Effect of calcitonin on gastrointestinal regulatory peptides in man. Clin Endocrinol (Oxf) 22:655-660CrossRefGoogle Scholar
  306. Stief CG, Benard F, Bosch RJLH, Aboseif SR, Lue TF, Tanagho EA (1990) A possible role for calcitonin gene-related peptide in the regulation of the smooth muscle tone of the bladder and penis. J Urol 143:392-397PubMedGoogle Scholar
  307. Stolarsky-Fredman L, Leff SE, Klein ES, Crenshaw EB III, Yeakley J, Rosenfeld MG (1990) A tissue-specific enhancer in the rat-calcitonin/CGRP gene is active in both neural and endocrine cell types. Mol Endocrinol 4:497-504PubMedCrossRefGoogle Scholar
  308. Struthers AD, Brown MJ, MacDonald DWR, Beacham JL, Stevenson JC, Morris HR, MacIntyre I (1986) Human calcitonin gene-related peptide: a potent endogenous vasodilator in man. Clin Sci 70:389-393PubMedGoogle Scholar
  309. Su HC, Bishop AE, Power RF, Hamada Y, Polak JM (1987) Dual intrinsic and extrinsic origins of CGRP- and NPY-immunoreactive nerves of rat gut and pancreas. J Neurosci 7:2674-2687PubMedGoogle Scholar
  310. Takamori M, Yoshikawa H (1989) Effect of calcitonin gene-related peptide on skeletal muscle via specific binding site and G protein. J Neurol Sci 90:99–109PubMedCrossRefGoogle Scholar
  311. Tannenbaum GS, Goltzman D (1985) Calcitonin gene-related peptide mimics calcitonin actions in brain on growth hormone release and feeding. Endocrinology 116:2685-2687PubMedCrossRefGoogle Scholar
  312. Tashjian AH Jr, Wright DR, Ivey JL, Pont A (1978) Calcitonin binding sites in bone: relationships to biological response and “escape”. Recent Prog Horm Res 34:285-334PubMedGoogle Scholar
  313. Tobler PH, Tschopp FA, Dambacher MA, Fischer JA (1984) Salmon and human calcitonin-like peptides in man. Clin Endocrinol (Oxf) 20:253-259CrossRefGoogle Scholar
  314. Tschirhart E, Bertrand C, Theodorsson E, Landry Y (1990) Evidence for the involvement of calcitonin gene-related peptide in the epithelium-dependent contraction of guinea-pig trachea in response to capsaicin. Naunyn Schmiede- bergs Arch Pharmacol 342:177-181CrossRefGoogle Scholar
  315. Tschopp FA, Tobler PH, Born W, Dambacher MA, Pettengill OS, Sorenson G, Fischer JA (1982) Human calcitonin and carboxyl-terminal adjacent peptide (PDN-21): identification of a common polyprotein in man. In: Cohn DV, Potts JT Jr, Fujita T (eds) Endocrine control of bone and calcium metabolism, Vol 8B. Elsevier, Amsterdam, pp 273-275Google Scholar
  316. Tschopp FA, Tobler PH, Fischer JA (1984) Calcitonin gene-related peptide in the human thyroid, pituitary and brain. Mol Cell Endocrinol 36:53-57PubMedCrossRefGoogle Scholar
  317. Tschopp FA, Henke H, Petermann JB, Tobler PH, Janzer R, Hökfelt T, Lundberg JM, Cuello C, Fischer JA (1985) Calcitonin gene-related peptide and its binding sites in the human central nervous system and pituitary. Proc Natl Acad Sci USA 82:248-252PubMedCrossRefGoogle Scholar
  318. Twery MJ, Kirkpatrick B, Critcher EC, Lewis MH, Mailman RB, Cooper CW (1986) Motor effects of calcitonin administered intracerebroventricularly in the rat. Eur J Pharmacol 121:189-198PubMedCrossRefGoogle Scholar
  319. Uchida S, Yamamoto H, Iio S, Matsumoto N, Wang X-B, Yonehara N, Imai Y, Inoki R, Yoshida H (1990) Release of calcitonin gene-related peptide-like immunoreactive substance from neuromuscular junction by nerve excitation and its action on striated muscle. J Neurochem 54:1000-1003PubMedCrossRefGoogle Scholar
  320. Umeda Y, Arisawa M (1989) Characterization of the calcitonin gene-related peptide receptor in mouse T lymphocytes. Neuropeptide 14:237-242CrossRefGoogle Scholar
  321. Umeda Y, Okada T (1987) Inhibition of gastric acid secretion by human calcitonin gene-related peptide with picomolar potency in guinea-pig parietal cell prepara- tions. Biochem Biophys Res Commun 146:430-436PubMedCrossRefGoogle Scholar
  322. Van Mansfeld ADM, Mosselman S, Höppener JWM, Zandberg J, van Teeffelen HAAM, Baas PD, Lips CJM, Jansz HS (1990) Islet amyloid polypeptide: structure and upstream sequences of the IAPP gene in rat and man. Biochim Biophys Acta 1087:235-240PubMedGoogle Scholar
  323. Venesio T, Mulatero B, Fasolo A (1987) Coexistence of substance P and calcitonin gene-related peptide in the frog spinal cord. Neurosci Lett 80:246-250PubMedCrossRefGoogle Scholar
  324. Villar MJ, Roa M, Huchet M, Hökfelt T, Changeux J-P, Fahrenkrug J, Brown JC, Epstein M, Hersh L (1989) Immunoreactive calcitonin gene-related peptide, vasoactive intestinal polypeptide, and somatostatin in developing chicken spinal cord motoneurons. Distribution and role in regulation of cAMP in cultured muscle cells. Eur J Neurosci 1:269-287PubMedCrossRefGoogle Scholar
  325. Villarreal D, Freeman RH, Verbürg KM, Brands MW (1988) Renal hemodynamic response to intrarenal infusion of calcitonin gene-related peptide in dogs. Peptides 9:1129-1135PubMedCrossRefGoogle Scholar
  326. Voelkel EF, Tashjian AH Jr, Davidoff FF, Cohen RB, Perlia CP, Wurtman RJ (1973) Concentrations of calcitonin and catecholamines in pheochromocytomas, a mucosal neuroma and medullary thyroid carcinoma. Endocrinol Metab 37: 297-307CrossRefGoogle Scholar
  327. Vora NM, Williams GA, Hargis GK, Bowser EN, Kawahara W, Jackson BL, Henderson WJ, Kukreja SC (1978) Comparative effect of calcium and of the adrenergic system on calcitonin secretion in man. J Clin Endocrinol Metab 46:567-571PubMedCrossRefGoogle Scholar
  328. Wada C, Hashimoto C, Kameya T, Yamaguchi K, Ono M (1988) Developmentally regulated expression of the calcitonin gene-related peptide (CGRP) in rat lung endocrine cells. Virchows Arch [B] 55:217-223Google Scholar
  329. Westermark P, Wernstedt C, Wilander E, Sletten K (1986) A novel peptide in the calcitonin gene-related peptide family as an amyloid fibril protein in the endocrine pancreas. Biochem Biophys Res Commun 140:827-831PubMedCrossRefGoogle Scholar
  330. Wiesenfeld-Hallin Z, Hökfelt T, Lundberg JM, Forssmann WG, Reinecke M, Tschopp FA, Fischer JA (1984) Immunoreactive calcitonin gene-related peptide and substance P coexist in sensory neurons to the spinal cord and interact in spinal behavioral responses of the rat. Neurosci Lett 52:199-204PubMedCrossRefGoogle Scholar
  331. Wimalawansa SJ, Morris HR, MacIntyre I (1989) Both α- and β-calcitonin gene- related peptides are present in plasma, cerebrospinal fluid and spinal cord in man. J Mol Endocrinol 3:247-252PubMedCrossRefGoogle Scholar
  332. Wimalawansa SJ, Morris HR, Etienne A, Blench I, Panico M, MacIntyre I (1990) Isolation, purification and characterization of β-hCGRP from human spinal cord. Biochem Biophys Res Commun 167:993-1000PubMedCrossRefGoogle Scholar
  333. Wohlwend A, Malmström K, Henke H, Murer H, Vassalli J-D, Fischer JA (1985) Calcitonin and calcitonin gene-related peptide interact with the same receptor in cultured LLC-PK1 kidney cells. Biochem Biophys Biophys Res Commun 131:537-542CrossRefGoogle Scholar
  334. Woloszczuk W, Reich-Kilcher B, Benke A, Dinstl K (1986) Effect of infusion of salmon calcitonin on the secretion of somatostatin and gastrin in man. Horm Metab Res 18:197-200PubMedCrossRefGoogle Scholar
  335. Yamamoto A, Kimura S, Hasui K, Fujisawa Y, Tamaki T, Fukui K, Iwao H, Abe Y (1988) Calcitonin gene-related peptide (CGRP) stimulates the release of atrial natriuretic peptide (ANP) from isolated rat atria. Biochem Biophys Res Commun 155:1452-1458PubMedCrossRefGoogle Scholar
  336. Yamatani T, Kadowaki S, Chiba T, Abe H, Chihara K, Fukase M, Fujita T (1986) Calcitonin gene-related peptide stimulates somatostatin release from isolated perfused rat stomach. Endocrinology 118:2144-2145PubMedCrossRefGoogle Scholar
  337. Yusufi ANK, Berndt TJ, Murayama N, Knox FG, Dousa TP (1987) Calcitonin inhibits Na+ gradient-dependent phosphate uptake across renal brush-border membranes. Am J Physiol 252:F598-F604PubMedGoogle Scholar
  338. Zabel M, Schäfer HJ (1988) Localization of calcitonin and calcitonin gene-related peptide mRNAs in rat parafollicular cells by hybridocytochemistry. J Histochem Cytochem 36:543-546PubMedCrossRefGoogle Scholar
  339. Zaidi M, Bevis PJR, Girgis SI, Lynch C, Stevenson JC, MacIntyre I (1985) Circulating CGRP comes from the perivascular nerves. Eur J Pharmacol 117:283-284PubMedCrossRefGoogle Scholar
  340. Zaidi M, Datta HK, Chambers TJ, MacIntyre I (1989) Production and characterisation of immunoreactive calcitonin gene-related peptide (CGRP) from a CGRP receptor-positive cloned osteosarcoma cell line (UMR 106.01). Biochem Biophys Res Commun 158:214-219PubMedCrossRefGoogle Scholar
  341. Zaidi M, Datta HK, Moonga BS, MacIntyre I (1990) Evidence that the action of calcitonin on rat osteoclasts is mediated by two G proteins acting via separate post-receptor pathways. J Endocrinol 126:473-481PubMedCrossRefGoogle Scholar
  342. Zhou Z-C, Villanueva ML, Noguchi M, Jones SW, Gardner JD, Jensen RT (1986) Mechanism of action of calcitonin gene-related peptide in stimulating pancreatic enzyme secretion. Am J Physiol 251:G391-G397PubMedGoogle Scholar
  343. Zona C, Farini D, Palma E, Eusebi F (1991) Modulation of voltage-activated channels by calcitonin gene-related peptide in cultured rat neurones. J Physiol (Lond) 433:631-643Google Scholar

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© Springer-Verlag Berlin Heidelberg 1993

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  • W. Born
  • J. A. Fischer

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