Selection of Functional RNA and DNA Molecules from Randomized Sequences
In vitro selection is a technique that allows the rapid screening of very large numbers of different DNA or RNA sequences for specific functional properties (Green et al. 1991; Famulok and Szostak 1992b; Szostak 1992). Applications of this method include the evaluation of binding sites between proteins and double-stranded (ds) or single-stranded (ss) nucleic acids, the study of structure, function, and substrate specificity of ribozymes and the de novo isolation and evaluation of nucleic acids (RNA and DNA) that bind to small biological and abiotic organic molecules.
KeywordsTriple Helix Primer Binding Site Selection Cycle Peptidyl Transferase Transcriptional Activator Protein
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- Famulok M, Szostak JW (1992b) In vitro selection of specific ligand-binding nucleic acids. Angew Chem 104: 1001–1011 (Angew Chem Int Ed Engl 104:979-988)Google Scholar