Identifying Cell-Cell Adhesion-Inhibitory Antibodies to Cell Surface Proteins Using Divalent Primary and Monovalent Secondary Antibodies — a Method Developed Using Cellular Slime Molds

  • W. R. Springer
Part of the Springer Laboratory book series (SLM)


Cellular slime mold has been used as a model for many developmental processes including cell-cell and species-specific adhesion. The organism is the subject of a number of books and countless research articles. The reader is referred to Loomis (1982), Raper (1984) and Spudich (1987) for three reviews covering all aspects of the cellular slime molds from their taxonomy to the isolation of specific proteins.


Dictyostelium Discoideum Cellular Slime Mold Conical Centrifuge Tube Percent Adhesion Incubation Tube 
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  2. Loomis WF (ed) (1982) The development of Dictyostelium discoideum . Academic Press, New YorkGoogle Scholar
  3. McDonough JP, Springer WR, Barondes SH (1980) Species-specific cell cohesion in cellular slime molds. Exp Cell Res 125: 1–14PubMedCrossRefGoogle Scholar
  4. Raper KB (1984) The dictyostelids. Princeton University Press PrincetonGoogle Scholar
  5. Springer WR, Barondes SH (1980) Cell adhesion molecules: detection with univalent second antibody. J Cell Biol 87: 703–707PubMedCrossRefGoogle Scholar
  6. Spudich J A (ed) (1987) Dictyostelium discoideum: molecular approaches to cell biology. Academic Press Orlando (Methods in cell biology, vol 28Google Scholar

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© Springer-Verlag Berlin Heidelberg 1993

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  • W. R. Springer

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