Identifying Cell-Cell Adhesion-Inhibitory Antibodies to Cell Surface Proteins Using Divalent Primary and Monovalent Secondary Antibodies — a Method Developed Using Cellular Slime Molds
Cellular slime mold has been used as a model for many developmental processes including cell-cell and species-specific adhesion. The organism is the subject of a number of books and countless research articles. The reader is referred to Loomis (1982), Raper (1984) and Spudich (1987) for three reviews covering all aspects of the cellular slime molds from their taxonomy to the isolation of specific proteins.
KeywordsDictyostelium Discoideum Cellular Slime Mold Conical Centrifuge Tube Percent Adhesion Incubation Tube
Unable to display preview. Download preview PDF.
- Gerisch G (1980) Univalent antibody fragments as tools for the analysis of cell interactions in Dictyostelium . In: Moscona AA, Monroy A (eds) Current topics in developmental biology. Academic Press New York pp 243–269Google Scholar
- Loomis WF (ed) (1982) The development of Dictyostelium discoideum . Academic Press, New YorkGoogle Scholar
- Raper KB (1984) The dictyostelids. Princeton University Press PrincetonGoogle Scholar
- Spudich J A (ed) (1987) Dictyostelium discoideum: molecular approaches to cell biology. Academic Press Orlando (Methods in cell biology, vol 28Google Scholar