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Lectins and Glycobiology pp 433–440Cite as

Identifying Cell-Cell Adhesion-Inhibitory Antibodies to Cell Surface Proteins Using Divalent Primary and Monovalent Secondary Antibodies — a Method Developed Using Cellular Slime Molds

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Abstract

Cellular slime mold has been used as a model for many developmental processes including cell-cell and species-specific adhesion. The organism is the subject of a number of books and countless research articles. The reader is referred to Loomis (1982), Raper (1984) and Spudich (1987) for three reviews covering all aspects of the cellular slime molds from their taxonomy to the isolation of specific proteins.

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References

  • Gerisch G (1980) Univalent antibody fragments as tools for the analysis of cell interactions in Dictyostelium . In: Moscona AA, Monroy A (eds) Current topics in developmental biology. Academic Press New York pp 243–269

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  • Loomis WF (ed) (1982) The development of Dictyostelium discoideum . Academic Press, New York

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  • McDonough JP, Springer WR, Barondes SH (1980) Species-specific cell cohesion in cellular slime molds. Exp Cell Res 125: 1–14

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  • Raper KB (1984) The dictyostelids. Princeton University Press Princeton

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  • Springer WR, Barondes SH (1980) Cell adhesion molecules: detection with univalent second antibody. J Cell Biol 87: 703–707

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  • Spudich J A (ed) (1987) Dictyostelium discoideum: molecular approaches to cell biology. Academic Press Orlando (Methods in cell biology, vol 28

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Authors
  1. W. R. Springer
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Editors and Affiliations

  1. Institut für Physiologische Chemie, Ludwig-Maximilians-Universität, Veterinärstraße 13, D-80539, München, Germany

    Hans-Joachim Gabius  & Sigrun Gabius  & 

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© 1993 Springer-Verlag Berlin Heidelberg

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Springer, W.R. (1993). Identifying Cell-Cell Adhesion-Inhibitory Antibodies to Cell Surface Proteins Using Divalent Primary and Monovalent Secondary Antibodies — a Method Developed Using Cellular Slime Molds. In: Gabius, HJ., Gabius, S. (eds) Lectins and Glycobiology. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77944-2_47

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  • DOI: https://doi.org/10.1007/978-3-642-77944-2_47

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77946-6

  • Online ISBN: 978-3-642-77944-2

  • eBook Packages: Springer Book Archive

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