Lectin and Neoglycoprotein Binding to Cells
Lectins and neoglycoproteins have been used extensively in histochemistry, electron microscopy, and flow cytometry. Many techniques exist for each application, utilizing a variety of labels and instrumentation. For example, the use of lectins for light microscopic staining of cells can involve fluorochromes, enzymes, or gold labels and can be accomplished by direct, indirect, sandwich, or avidin-biotin methods (McCoy 1987). Comprehensive surveys of histochemical and cytochemical staining procedures are found in several excellent reviews (Schrevel et al. 1981; Roth 1983; Leathern 1986) and in this book (Danguy and Gabius; Egea; Kolb-Bachofen).
KeywordsColloidal Gold Trisodium Citrate Gold Marker Boar Sperm Bovine Spermatozoon
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