Advertisement

Neoglycoprotein-Gold Complexes as a Tool in the Study of Carbohydrate-Specific Binding Proteins

  • V. Kolb-Bachofen
  • C. Egenhofer
Part of the Springer Laboratory book series (SLM)

Abstract

Labeling of ligands or antibodies by using colloidal gold particles has become a widely used technique. Initially, this technique was introduced into electron microscopy exploiting the electron density of these particles (Faulk and Taylor 1971; Horrisberger and von Lanthen 1979). Since then, gold labeling has in addition been applied in Western or dot blots (Brada and Roth 1984), in photometric measurements (Teradeira et al. 1983), and in light microscopy (Geoghegan et al. 1978; Holgate et al. 1983) also in connection with in situ hybridization experiments (Wolber and Beals 1989). The latter applications exploit the dark red color of the colloids and the possibility to increase the signal by the silver enhancement technique (Moeremans et al. 1984), which has led to a very high sensitivity, so that in Western blots detection levels are in the picogram range (Holgate et al. 1983; Moeremans et al. 1984; Springall et al. 1984).

Keywords

Gold Particle Colloidal Gold Gold Complex Colloidal Gold Particle Gold Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alsdorff K, Kolb-Bachofen V (1992) Isolation and purification of a receptor for C-reactive protein (CRP) from rat macrophages. Eur J cell Biol 57 (Suppl 36): 5Google Scholar
  2. Baschong W, Lucocq JM, Roth J (1985) Thiocyanate gold: small (2–3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy. Histochemistry 83: 409–411PubMedCrossRefGoogle Scholar
  3. Brada D, Roth J (1984) “Golden Blot”-detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes. Anal Biochem 142: 79–83PubMedCrossRefGoogle Scholar
  4. De Waele M, De Mey J, Renmans W, Labeur C, Reynaert PH, Van Camp B (1986) An immunogold-silver staining method for detection of cell-surface antigens in light microscopy. J Histochem Cytochem 34: 935–939PubMedCrossRefGoogle Scholar
  5. Faulk WP, Taylor GM (1971) An immunocolloid method for the electron microscope. Immunochemistry 8: 1081–1083PubMedCrossRefGoogle Scholar
  6. Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisoperse gold solutions. Nature (London) Phys Sci 241: 20–22Google Scholar
  7. Geoghegan WD, Ackerman GA (1977) Adsorption of horseradish peroxidase, ovomucoid and antiimmunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem 25: 1187–1200PubMedCrossRefGoogle Scholar
  8. Geoghegan WD, Scillian JJ, Ackerman GA (1978) The detection of human B lymphocytes by both light and electron microscopy utilizating colloidal gold labeled anti-immunoglobulin. Immunol Commun 7: 1–12PubMedGoogle Scholar
  9. Handley DA, Chien S (1987) Colloidal gold labeling studies related to vascular and endothelial functions, hemostasis and receptor-mediated processing of plasma macromolecules. Eur J Cell Biol 43: 163–174PubMedGoogle Scholar
  10. Holgate CS, Jackson R, Cowen PN, Bird CC (1983) Immunogold-silver staining: new method for immuno-staining with enhanced sensitivity. J Histochem Cytochem 31: 938–944PubMedCrossRefGoogle Scholar
  11. Horrisberger M (1979) Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy. Biol Cell 36: 253–258Google Scholar
  12. Horrisberger M, von Lanthen M (1979) Multiple marking of cell surface receptors by gold granules: simultaneous localization of three lectin receptors on human erythrocytes. J Microscopy (Oxford) 115: 97–102CrossRefGoogle Scholar
  13. Hsu YH (1984) Immunogold for detection of antigen on nitrocellulose paper. Anal Biochem 142:221–22PubMedCrossRefGoogle Scholar
  14. Kolb-Bachofen V (1989) Carbohydrate receptor binding using colloidal gold. In: Ginsburg V (ed) Methods in enzymology, vol 179. Academic Press, London New York, pp 111–121Google Scholar
  15. Lee RT, Lee YC (1980) Preparation and some biochemical properties of neoglycoproteins produced by reductive amination thioglycosides containing an co-aldehydoaglycon. Biochemistry 19: 156–163PubMedCrossRefGoogle Scholar
  16. Moeremans M, Daneels G, Van Dijck A, Langanger G, De Mey J (1984) Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immuno-gold/silver staining. J Immunol Methods 74: 353–360PubMedCrossRefGoogle Scholar
  17. Roth J (1983a) Applications of immunocolloids in light microscopy. II. Demonstration of lectin-binding sites in paraffin sections by the use of lectin-gold or glycoprotein-gold complexes. J Histochem Cytochem 31: 547–552PubMedCrossRefGoogle Scholar
  18. Roth J (1983b) The colloidal gold marker system for light and electron microscopic cytochemistry. In: Bulloch GR, Petrusz P (eds) Techniques in immunocytochemistry, vol 2. Academic Press, New York, pp 217–284Google Scholar
  19. Springall DR, Hacker GW, Grimelius L, Polak JM (1984) The potential of the immunogold-silver staining method for paraffin sections. Histochemistry 81: 603–608PubMedCrossRefGoogle Scholar
  20. Teradeira R, Kolb-Bachofen V, Schlepper-Schafer J, Kolb H (1983) Galactose-particle receptor on liver macrophages. Quantition of particle uptake. Biochem Biophys Acta 759: 306–310CrossRefGoogle Scholar
  21. Wolber RA, Beals TF (1989) Streptavidin-gold labeling for ultrastructural in situ nucleic acid hybridization. In: Hayat MA (ed) Colloidal gold: principles, methods, and applications, vol 2. Academic Press, London New York, pp 379–396Google Scholar
  22. Zsigmondy R (1901) Z Anal Chem 40: 697–719CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • V. Kolb-Bachofen
  • C. Egenhofer

There are no affiliations available

Personalised recommendations