Neoglycoprotein-Gold Complexes as a Tool in the Study of Carbohydrate-Specific Binding Proteins
Labeling of ligands or antibodies by using colloidal gold particles has become a widely used technique. Initially, this technique was introduced into electron microscopy exploiting the electron density of these particles (Faulk and Taylor 1971; Horrisberger and von Lanthen 1979). Since then, gold labeling has in addition been applied in Western or dot blots (Brada and Roth 1984), in photometric measurements (Teradeira et al. 1983), and in light microscopy (Geoghegan et al. 1978; Holgate et al. 1983) also in connection with in situ hybridization experiments (Wolber and Beals 1989). The latter applications exploit the dark red color of the colloids and the possibility to increase the signal by the silver enhancement technique (Moeremans et al. 1984), which has led to a very high sensitivity, so that in Western blots detection levels are in the picogram range (Holgate et al. 1983; Moeremans et al. 1984; Springall et al. 1984).
KeywordsPhosphorus Ethyl Albumin Carbohydrate Citrate
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