Skip to main content
SpringerLink
Log in

Molekulare Aspekte der Invasion von Krebszellen

  • Conference paper
Immuntherapie in der Uroonkologie

  • 24 Accesses

Zusammenfassung

Einerseits werden Invasion und Metastasierung durch die Hinaufregulation der Expression der kritischen Komponenten gefördert; diese können als Produkte dominanter Gene betrachtet werden. Proteasen (Kathepsine, Kollagenasen, Plasminogenaktivatoren usw.) sind erste Kandidaten in dieser Gruppe (Basset et al. 1990), da diese Moleküle den für invadierende Tumorzellen notwendigen Raum schaffen können. Auch Zelladhäsionsmo-leküle und Lektine sind beteiligt, da die invadierenden Zellen auf ihrem Wanderpfad ständig neue Kontakte herstellen. Zum Beispiel wurde eine gespleißte Variante des Zelladhäsionsmoleküls CD44 entdeckt, die nach Transfektion der entsprechenden cDNS in niedrig-metastatische Zellen das metastatische Potential erhöht (Günthert et al. 1991). Motilitäts- und Wachstumsfaktoren, welche die Zellmotilität beeinflussen, können auch auf das Invasionsverhalten Auswirkungen haben. Zum Beispiel fördern der Scatterfaktor und der Fibroblastenwachstumsfaktor die Invasivität von Epithelzellen in vitro (Weichner et al. 1990; Jouanneau et al. 1991). Die cDNS für den Rezeptor des autokrinen Motilitätsfaktors (AMF), der an der Progression von Harnblasenkarzinomen beteiligt zu sein scheint, wurde bereits charakterisiert; das kodierte Protein weist eine Homologie zu p53 auf (Watanabe et al. 1991).

Übersetzung aus dem Engl. von Belinde Junkers.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 54.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 69.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  • Basset G, Bellocq JP, Wolf C et al. (1990) A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature 348: 699–704

    Article  PubMed  CAS  Google Scholar 

  • Behrens J, Birchmeier W, Goodman SL, Imhof BA (1985) Dissociation of Madin-Darby canine kidney epithelial cells by the monoclonal antibody anti-Arc-1: mechanistic aspects and identification of the antigen as a component related to uvomorulin. J Cell Biol 101: 1307–1315

    Article  PubMed  CAS  Google Scholar 

  • Behrens J, Mareel MM, Van Roy FM, Birchmeier W (1989) Dissecting tumor cell invasion: Epithelial cells acquire invasive properties after the loss of unvomorulin-mediated cell-cell adhesion. J Cell Biol 108: 2435–2447

    Article  PubMed  CAS  Google Scholar 

  • Behrens J, Löwrick O, Klein-Hitpass L, Birchmeier W (1991) The E-cadherin promoter: Functional analysis of a GC-rich region and an epithelial specific palindromic regulator element. Proc Natl Acad Sci USA 88: 11495–11499

    Article  PubMed  CAS  Google Scholar 

  • Boller K, Vestweber D, Kemler R (1985) Cell-adhesion molecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells. J Cell Biol 100: 327–332

    Article  PubMed  CAS  Google Scholar 

  • Bottaro DP, Rubin JS, Faletto DL et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science 251: 802–904

    Article  PubMed  CAS  Google Scholar 

  • Carter BS, Ewing CM, Ward WS et al. (1990) Allelic loss of chromosome 16q and 10q in human prostate cancer. Proc Natl Acad Sci USA 87: 8751–8755

    Article  PubMed  CAS  Google Scholar 

  • Edelman GM (1984) Cell adhesion and morphogenesis: The regulator hypothesis. Proc Natl Acad Sci USA 81: 1460–1464

    Article  PubMed  CAS  Google Scholar 

  • Fearon ER, Cho KR, Nigro JM et al. (1990) Identification of a chromosome 18q gene that is altered in colorectal cancers. Science 247: 49–56

    Article  PubMed  CAS  Google Scholar 

  • Frixen U, Behrens J, Sachs M et al. (1991) E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells. J Cell Biol 111: 173–185

    Article  Google Scholar 

  • Gherardi E, Stoker M (1990) Scatter factor and hepatocytes. Nature 346: 228 (lett)

    Article  PubMed  CAS  Google Scholar 

  • Giancotti FG, Ruoslathi E (1990) Elevated levels of the 5ßl fibronectin receptor suppresses the transformed phenotype of Chinese hamster ovary cells. Cell 60: 849–859

    Article  PubMed  CAS  Google Scholar 

  • Grey A-M, Schor AM, Rushton G, Ellis I, Schor SL (1989) Purification of the migration stimulating factor produced by fetal and breast cancer patient fibroblasts. Proc Natl Acad Sci USA 86: 2438–2442

    Article  PubMed  CAS  Google Scholar 

  • Günthert U, Hoffmann M, Rudy W et al. (1991) A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 65: 13–24

    Article  PubMed  Google Scholar 

  • Jouanneau J, Gavrilowic J, Cavuelle D, Jaye M, Moens G, Cavuelle J-P, Thiery JP (1991) Secreted or non-secreted forms of acid fibroblast growth factor produced by transfected epithelial cells influence cell morphology, motility, and invasive potential. Proc Natl Acad Sci USA 88: 2893–2997

    Article  PubMed  CAS  Google Scholar 

  • Koch PJ, Walsh MJ, Schmelz M, Goldschmidt MD, Zimbelman R, Franke WW (1990) Identification of desmoglein, a constitutive desmosomal glycoprotein as a member of the Cadherin family of cell adhesion molecules. Eur J Cell Biol 53: 1–12

    PubMed  CAS  Google Scholar 

  • Leask A, Rosenberg M, Vassar R, Fuchs E (1990) Regulation of a human epidermal keratin gene: Sequences and nuclear factors involved in keratinocyte-specific transcription. Genes Dev 4: 1985–1998

    Article  PubMed  CAS  Google Scholar 

  • Lee SW, Tomasetto C, Sager R (1991) Positive selection of candidate tumor-suppressor genes by subtractive hybridization. Proc Natl Acad Sci USA 88: 2825–2829

    Article  PubMed  CAS  Google Scholar 

  • Leone A, Flatow U, King CR, Sandeen MA, Margulis IMK, Liotta LA, Steeg RS (1991) Reduced tumor incidence, metastatic potential, and cytokine responsiveness of nm 23-transfected melanoma cells. Cells 65: 25–35

    Article  CAS  Google Scholar 

  • Liotta LA, Mandler R, Murano G, Katz DA, Gordon RK, Ching PK, Schiffmann E (1986) Tumor cell autocrine motility factor. Proc Natl Acad Sci USA 83: 3302–3306

    Article  PubMed  CAS  Google Scholar 

  • Mareel MM (1983) Invasion in vitro: Methods of analysis. Cancer Metastasis Rev 2: 201–218

    Article  PubMed  CAS  Google Scholar 

  • Mechanic S, Raynor K, Hill JE, Cowin P (1991) Desmocollins form a distinct subset of the Cadherin family of cell adhesion molecules. Proc Natl Acad Sci USA 88: 4476–4480

    Article  PubMed  CAS  Google Scholar 

  • Mege RM, Matsuzaki F, Gallin WJ, Goldberg JI, Cunningham BA, Edelman GM (1988) Construction of epithelioid sheets by transfection of mouse sarcoma cells with cDN As for chicken cell adhesion molecules. Proc Natl Acad Sci USA 85: 7274–7278

    Article  PubMed  CAS  Google Scholar 

  • Miyazawa K, Tsubouchi H, Naka D et al. (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor. Biochem Biophys Res Commun 163: 967–973

    Article  PubMed  CAS  Google Scholar 

  • Nagafuchi A, Shirayoshi Y, Okazaki K, Yasuda K, Takeichi M (1987) Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. Nature (London) 329: 341–343

    Article  CAS  Google Scholar 

  • Naldini L, Weidner KM, Vigna E et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J 2867–2878

    Google Scholar 

  • Nakamura T, Nishizawa T, Hagiya M et al. (1989) Molecular cloning and expression of human hepatocyte growth factor. Nature 342: 440–443

    Article  PubMed  CAS  Google Scholar 

  • Natt E, Magenis RE, Zimmer J, Mansouri A, Scherer G (1989) Regional assignment of the human loci for unvomorulin (UVO) and chymotrypsinogen B (CTRB) with the help of two overlapping deletions on the long arm of chromosome 16. Cytogenet Cell Genet 50: 145–148

    Article  PubMed  CAS  Google Scholar 

  • Ringwald M, Schuh R, Vestweber D et al. (1987) The structure of the cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion. EMBO J 6: 3647–3653

    PubMed  CAS  Google Scholar 

  • Rubin JS, Osada H, Finch PW, Taylor WG, Rudikoff S, Aaronson SA (1991) Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc Natl Acad Sci USA 86: 802–806

    Article  Google Scholar 

  • Sato T, Tanigami A, Yamakawa K, Akiyama F, Kasumi F, Sakamoto G, Nakamura Y (1990) Allelotype of breast cancer: cummulative allele losses promote tumor progression in primary breast cancer. Cancer Res 50: 7184–7189

    PubMed  CAS  Google Scholar 

  • Schipper JH, Frixen UH, Behrens J, Unger A, Jahncke K, Birchmeier W (1991) E-cadherin expression in squamous cell carcinomas of head and neck: Inverse correlation with tumor differentiation and lymph node metastasis. Cancer Res 51: 6328–6337

    PubMed  CAS  Google Scholar 

  • Shimoyama Y, Hirohashi S (1991) Expression of E- and P-cadherin in gastric carcinomas. Cancer Res 51: 2185–2192

    PubMed  CAS  Google Scholar 

  • Stoker M, Gherardi E, Perryman M, Gray J (1987) Scatter factor is a fibroblast-derived modulator of epithelial cell motility. Nature 327: 239–242

    Article  PubMed  CAS  Google Scholar 

  • Takeichi M (1991) Cadherin cell adhesion receptors as a morphologic regulator. Science 251: 1451–1455

    Article  PubMed  CAS  Google Scholar 

  • Tsuda H, Zhang W, Shimosato Y et al. (1990) Allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma. Proc Natl Acad Sci USA 87: 6791–6794

    Article  PubMed  CAS  Google Scholar 

  • Vleminckx K, Vakat L Jr, Mareel M, Fiers W, Van Roy F (1991) Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role. Cell 66: 107–119

    Article  PubMed  CAS  Google Scholar 

  • Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A (1991) Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor. J Biol Chem 266: 13442–13448

    PubMed  CAS  Google Scholar 

  • Weidner KM, Behrens J, Vandekerckhove J, Birchmeier W (1990) Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells. J Cell Biol 111: 2097–2108

    Article  PubMed  CAS  Google Scholar 

  • Weidner KM, Arakaki N, Hartmann G et al. (1991) Evidence for the identity of human scatter factor and human hepatocyte growth factor. Proc Natl Acad Sci USA 88: 7001–7005

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. W. Birchmeier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. M. Weidner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Schipper
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Behrens
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Urologische Klinik und Poliklinik, Universitätsklinikum Essen, Hufelandstr. 55, W-4300, Essen 1, Deutschland

    Herbert Rübben  & Mark Goepel  & 

  2. Urologische Universitätsklinik, Moorenstr. 5, W-4000, Düsseldorf 1, Deutschland

    Bernd J. Schmitz-Dräger

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Birchmeier, W., Weidner, K.M., Schipper, J., Behrens, J. (1993). Molekulare Aspekte der Invasion von Krebszellen. In: Rübben, H., Goepel, M., Schmitz-Dräger, B.J. (eds) Immuntherapie in der Uroonkologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77830-8_10

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-77830-8_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77831-5

  • Online ISBN: 978-3-642-77830-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation