Age Differences in Human Skin Collagen

  • J. Brinckmann
  • M. Brey
  • P. K. Müller
Conference paper

Abstract

Aging of the skin must be divided into endogenous aging and photo aging. We investigated compositional differences in the collagens of (photo-) aged skin. The results were compared with the qualitative collagen synthesis of (photo-) aged fibroblasts. There is no difference in the relative proportion of type-Ill to type-I collagens in skin extracts and cell cultures from young and old sun-protected areas. In photo aged skin there is an increase of the extractable type III. This increase was not detected in cell culture. These differences may be explained by photo-induced changes during the process of cross-linking. Modified intermolecular cross-links influence the susceptibility of collagens to collagenase attack. UV-aged cells showed a higher collagenase production, so a slower cleavage of type-III collagen would result in an accumulation of this type in the tissue.

Keywords

Glutamine Polyacrylamide Streptomycin Fibril Collagenase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dalle Carbonare M, Pathok MA (1989) Photoaging and the role of UV (290–400 nm) and reactive oxygen species in collagen cross-links. J Invest Dermatol 92: 410Google Scholar
  2. 2.
    Duncan MR, Berman B (1989) Differential regulation of collagen, glycosaminoglycan, fibronectin, and collagenase activity production in cultured human adult dermal fibroblasts by interleukin 1-alpha and beta and tumor necrosis factor-alpha and beta. J Invest Dermatol 92: 699–706PubMedCrossRefGoogle Scholar
  3. 3.
    Houck CJ, Sharma VK, Hayflick L (1971) Functional failures of cultured human diploid fibroblasts after continued population doublings. Proc Soc Exp Biol Med 137: 331–333PubMedGoogle Scholar
  4. 4.
    Junker P, Lorenzen I (1983) Reversibility of D-penicillamin induced collagen alterations in rat skin and granulation tissue. Biochem Pharmacol 32 (11): 1753PubMedCrossRefGoogle Scholar
  5. 5.
    Lavker RM, Zhen P, Dong G (1987) Aged skin: a study by light, transmission- electron, and scanning-electron microscopy. J Invest Dermatol 88: 44s - 51sPubMedCrossRefGoogle Scholar
  6. 6.
    Schwartz T, Luger TA (1989) Effect of UV irridiation on epidermal cell cytokine production. J Photochem Photobiol 4: 1–13CrossRefGoogle Scholar
  7. 7.
    Tsuji T, Yorifuji T, Hayashi Y, Hamada T (1986) Light and scanning-electron microscopic studies on wrinkles in aged persons’ skin. Br J Dermatol 114: 329–335PubMedCrossRefGoogle Scholar
  8. 8.
    Uitto J (1989) Connective tissue biochemistry of the aging dermis: age-associated alterations in collagen and elastin. Clin Geriatr Med 5 (1): 127–147PubMedGoogle Scholar
  9. 9.
    Vater CA, Haus ED jr, Siegel RC (1979) Native cross-links in collagen fibrils induce resistance to human synovial collagenase. Biochem J 181: 639–645PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • J. Brinckmann
  • M. Brey
  • P. K. Müller

There are no affiliations available

Personalised recommendations