Are Rat Granulation Tissue Fibroblasts Regulated by Cytokines in the Same Manner as Skin Fibroblasts?
The aim of the present study was to compare the collagen synthesis and proliferation of fibroblasts from human and rat skin with those from granulation tissue before and after incubation with transforming growth factor (31 (TGFβ) and interferon-gamma (IFNg). TGFβand IFNg are well-characterized cytokines that are able to regulate fibroblast metabolism. We showed that rat skin and human skin fibroblasts did not differ in proliferation or in their capacity to synthesize collagen. However, rat granulation tissue fibroblasts seemed to be in an activated state. Their proliferation and their collagen synthesis were significantly increased compared with normal rat skin and human skin fibroblasts. This effect was significant in 3-day-old granulation tissue fibroblasts. The effects of the cytokines TGFβand IFNg on collagen synthesis and proliferation differed quantitatively between skin and granulation tissue fibroblasts. In reaction to the cytokines used, day-3 granulation tissue fibroblasts produced the highest levels of collagen, followed by day-6 granulation tissue fibroblasts. Our findings support the idea of a time-dependent activation of granulation tissue fibroblasts.
KeywordsSerine Proline Polypeptide Interferon Glutamine
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- 3.Clark RAF (1991) Cutaneous wound repair: a review with emphasis on integrin receptor expression. In: Janssen H, Rooman R, Robertson JIS (eds) Wound healing. Wirghtson:Google Scholar
- 15.Czaja MJ, Weiner FR, Eghbali ME, Giambrone M-A, Eghbali MA, Zern MA (1987) Differential effects of g-interferon on collagen and fibronectin gene expression. J Biol Chem 27: 13348–13351Google Scholar
- 18.Kulozik M, Heckmann M, Mauch C, Scharffetter K, Krieg T (1991) Cytokine regulation of collagen metabolism during wound healing in vitro and in vivo. In: Janssen H, Rooman R, Robertson JIS (eds) Wound healing. WirghtsonGoogle Scholar
- 24.Bayreuther K, Rodemann HP, Francz PI, Maier K (1988) Differentiation of fibroblasts stem cells. J Cell Sci [Suppl] 10: 115–130Google Scholar