Re-epithelialization of Incision and Burn Wounds in Culture
Sheets of epidermal cells emigrated from tadpoles of Xenopus laevis retain their in situ relationship of basal and surface cells. These cellular sheets have been used to compare the process of primary closure of wounds made either by incision with a scalpel or by UV irradiation. In the latter case, no response of the cells surrounding the killed epidermis is observed. After mechanical injury, retraction of the wound margins followed by extension of lamellae and subsequent migration of the marginal cells immediately initiates wound closure. We conclude that mechanical interactions of the intact cells with those being killed by irradiation prevents the sequence of reactions taking place in incision wounds.
KeywordsEpidermal Cell Xenopus Laevis Wound Closure Incision Wound Marginal Cell
Unable to display preview. Download preview PDF.
- 2.Bereiter-Hahn J (1986) Epidermal cell migration and wound repair. In: Bereiter- Hahn J, Matoltsy AG, Richards S (eds) Biology of the integument, vol. 2. Springer, Berlin Heidelberg New York, pp 443–471Google Scholar
- 3.Bereiter-Hahn J (1988) Involvement of microcompartmentation in the regulation of cell proliferation. In: Jones DP (ed) Microcompartmentation. CRC, Boca Raton, pp 56–69Google Scholar
- 10.Korohoda W (1971) Interrelations of motile and metabolic activities in tissue culture cells. I. Respiration in chicken embryo fibroblasts actively locomoting, contact-inhibited, and suspended in fluid medium. Folia Biol (Praha) 19: 41–51Google Scholar
- 13.Matsuda M, Sawa M, Edelhauser HF, Bartels SP, Neufeld AH, Kenyon KR (1985) Cellular migration and morphology in corneal endothelial wound repair. Invest Ophthalmol Sci 26: 443–449Google Scholar
- 14.Montandon D, Gabbiani G (1985) Contractile phenomena in burn wounds. In: May JF, Dogo J (eds), Care of the burn wound. Karger, Basel, pp 111–116Google Scholar
- 15.Nieuwkoop PD, Faber J (1956) Normal tables of Xenopus laevis (DAUDIN). North- Holland, AmsterdamGoogle Scholar
- 18.Takeuchi S (1972) Wound healing of the cornea in the chick embryo. I. Factors effecting the migration of corneal epithelium. J Fac Sci Univ Tokyo 12: 439 - 447Google Scholar
- 19.Wolfe K, Quimby MC (1962) Synthetic media. Amphibian culture medium. Excerpta Med 16 (9):Google Scholar