Metabolic Reactions: Mechanisms of Substrate Oxygenation

  • H. Rein
  • C. Jung
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 105)


A characteristic property of cytochrome P450 enzymes is their ability to react with a multitude of chemically very different substrates. Despite this fact, the basic mechanism of substrate oxygenation is the same for multiple forms and isozymes; it includes the energy-expensive process of molecular oxygen activation, splitting and formation of a very reactive oxygen species suited to oxidizing such chemically inert compounds as alkanes. The catalytic power of cytochrome P450 can be demonstrated by the thermodynamic parameters of the oxygenation process (Eq. 1).


Soret Band Microsomal Cytochrome Terminal Oxygen Atom Thiolate Ligand Deuterium Isotope Effect 
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© Springer-Verlag Berlin Heidelberg 1993

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  • H. Rein
  • C. Jung

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