Lanosterol 14α-Demethylase (Cytochrome P45014DM)

  • Y. Yoshida
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 105)


Lanosterol 14α-demethylase (cytochrome P45014DM) is a housekeeping enzyme occurring widely in eukaryotes. It is a cytochrome P450 mono-oxygenase catalyzing the conversion of lanosterol or 24,25-dihydrolanosterol (DHL) to the 14-demethylated and 14,15-desaturated derivatives by removing the 14α-methyl group (C32) as formic acid (Fig. 1) (Watkinson and Akhtar 1969; Alexander et al. 1972; Gibbons and Mitropoulos 1973; Mitropoulos et al. 1976; Aoyama and Yoshida 1978; Aoyama et al. 1984; Trzaskos et al. 1986). The 14α-demethylation is an essential reaction of steroid biosynthesis by eukaryotes, because most of the functional steroids have no 14-methyl group.


Heme Iron Cytochrome P450 Monooxygenases Ergosterol Biosynthesis Yeast Enzyme Azole Antifungal Agent 
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© Springer-Verlag Berlin Heidelberg 1993

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  • Y. Yoshida

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