Lanosterol 14α-Demethylase (Cytochrome P45014DM)
- 380 Downloads
Lanosterol 14α-demethylase (cytochrome P45014DM) is a housekeeping enzyme occurring widely in eukaryotes. It is a cytochrome P450 mono-oxygenase catalyzing the conversion of lanosterol or 24,25-dihydrolanosterol (DHL) to the 14-demethylated and 14,15-desaturated derivatives by removing the 14α-methyl group (C32) as formic acid (Fig. 1) (Watkinson and Akhtar 1969; Alexander et al. 1972; Gibbons and Mitropoulos 1973; Mitropoulos et al. 1976; Aoyama and Yoshida 1978; Aoyama et al. 1984; Trzaskos et al. 1986). The 14α-demethylation is an essential reaction of steroid biosynthesis by eukaryotes, because most of the functional steroids have no 14-methyl group.
KeywordsHeme Iron Cytochrome P450 Monooxygenases Ergosterol Biosynthesis Yeast Enzyme Azole Antifungal Agent
Unable to display preview. Download preview PDF.
- Ishida N, Aoyama Y, Hatanaka R, Oyama Y, Imajo S, Ishiguro M, Oshima T, Nakazato H, Noguchi T, Maitra US, Mohan VP, Sprinson DB, Yoshida Y (1988) A single amino acid substitution converts cytochrome P45014DM to an inactive form, cytochrome P450SG1-complete primary structures deduced from cloned DNAs. Biochem Biophys Res Commun 155: 317–323PubMedCrossRefGoogle Scholar
- Ortiz de Montellano PR (1986) Oxygen activation and transfer. In: Ortiz de Montellano (ed) Cytochrome P450: structure, mechanism and biochemistry. Plenum, New York, pp 217–272Google Scholar
- Watkinson IA, Akhtar M (1969) The formation of an 8,14-diene system during cholesterol biosynthesis. J Chem Soc Chem Commun 1969: 206Google Scholar
- Yoshida Y, Aoyama Y, Nishino T, Katsuki H, Maitra US, Mohan VP, Sprinson DB (1985) Spectral properties of a novel cytochrome P450 of a Saccharomyces cerevisiae mutant SG1. A cytochrome P450 species having a nitrogenous ligand trans to thiolate. Biochem Biophys Res Commun 127: 623–628PubMedCrossRefGoogle Scholar