Cytochrome P450: Probes of Active Site Residues

  • L. S. Kaminsky
  • R. S. Obach
  • M. J. Fasco
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 105)


The extent of the multiplicity of cytochrome P450 has been demonstrated by the current listing of 154 cytochrome P450 genes (Nebert et al. 1991). This multiplicity is to a major extent responsible for the extremely broad substrate specificity of the hepatic microsomal monooxygenase system, although many individual cytochromes P450 catalyze the metabolism of a number of substrates and frequently display overlapping substrate specificities (Guengerich et al. 1982; Yang and Lu 1987). In contrast to this broad substrate specificity of cytochrome P450 forms, some also display regio- and stereospecificities for selected substrates (Guengerich et al. 1982).


Active Site Residue Heme Cofactor Mammalian Cytochrome Photoaffinity Probe Cytochrome P450 Form 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • L. S. Kaminsky
  • R. S. Obach
  • M. J. Fasco

There are no affiliations available

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