Effective Reconstitution of Cell Membrane Proteins into Artificial Cell Liposomes

  • J. Sunamoto
  • V. Rosilio
  • Y. Mori
  • K. Suzuki
  • M. Ishitobi
  • T. Sato
  • K. Akiyoshi
Conference paper

Abstract

Perfect extraction of intrinsic membrane proteins or enzymes from intact cells and reconstitution of them without any denaturation and deactivation are basic requirement in investigation of the function of membrane proteins and/or the utilization of them in membrane protein engineering. For this purpose, artificial cell, liposome, seems to be a desirable and promising tool in both basic investigation and application.

Keywords

Cholesterol Sucrose Filtration Carbohydrate Leukemia 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. Sunamoto
    • 1
  • V. Rosilio
    • 1
  • Y. Mori
    • 1
  • K. Suzuki
    • 1
  • M. Ishitobi
    • 1
  • T. Sato
    • 1
  • K. Akiyoshi
    • 1
  1. 1.Department of Polymer ChemistryKyoto UniversityKyotoJapan

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