Abstract
B lineage cells in the bone marrow can be recognized by the expression of the high molecular weight form of the common leukocyte antigen CD45, known as B220. We showed recently [1, 2] that a small portion of these cells in bone marrow co-express leukosialin, CD43 [3, 4], and that many of these cells possessed D-J, but not V-D-J rearrangements. Further analysis with other markers revealed that this D-J rearranging population was contained in the fraction of B220+CD43+ cells that also expressed intermediate levels of the heat stable antigen, HSA. We showed that in short term culture, many of these cells would progress to the Pre-B and B cell stages. We have termed cells with this phenotype “Pro-B”. These Pro B cells can be detected both in bone marrow and fetal, known sources of B lymphopoiesis (Figure 1).
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References
Hardy RR, Kemp JD, Hayakawa K (1989) Analysis of lymphoid population in Sci.d mice; detection of a potential B lymphocyte progenitor population present at normal levels in Sci.d mice by three color flow cytometry with B220 and S7. Curr Top Microbiol Immunol 152:19–25.
Hardy RR, Carmack CE, Shinton SA, Kemp D, Hayakawa K (1991) Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow. J Exp Med 173:1213–1225.
Baecher CM, Infante AJ, Semcheski KL, Frelinger JG (1988) Identification and characterization of a mouse cell surface antigen with alternative molecular forms. Immunogenetics 28:295–302.
Gulley ML, Ogata LC, Thorson JA, Dailey O, Kemp JD (1988) Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation. J Immunol 140:3751–3757.
Hardy RR, Hayakawa K (1991) A developmental switch in B lymphopoiesis. Proc. Natl. Acad. Sci. USA 88:11550–11554.
Bosma MJ (1989) The Sci.d mutation: occurrence and effect. Curr Top Microbiol Immunol 152:3–9.
Hayakawa K, Hardy RR, Herzenberg LA, Herzenberg LA (1985) Progenitors for Ly-1 B cells are distinct from progenitors for other B cells. J Exp Med 161:1554–1568.
Boumsell L, Bernard A, Lepage V, Degos L, Lemerle J, Dausset J (1978) Some chronic lymphocytic leukemia cells bearing surface Immunoglobulins share determinants with T cells. Eur J Immunol 8:900–904.
Haughton G, Arnold LW, Bishop GA, Mercolino TJ (1986) The CH series of murine B cell lymphomas: neoplastic analogues of Ly-1+ normal B cells. Immunol Rev 93:35–51.
Hayakawa K, Hardy RR, Stall AM, Herzenberg A, Herzenberg LA (1986) Immunoglobulin-bearing B cells reconstitute and maintain the murine Ly-1 B cell lineage. Eur J Immunol 16:1313–1316.
Mercolino TJ, Arnold LW, Hawkins LA, Haughton G (1988) Normal mouse peritoneum contains a large population of Ly-1+ (CD5) B cells that recognize phosphatidyl choline. Relationship to cells that secrete hemolytic antibody specific for autologous erythrocytes. J Exp Med 168:687–698.
Hardy RR, Carmack CE, Shinton SA, Riblet J, Hayakawa K (1989) A single V H gene is utilized predominantly in anti-BrMRBC hybridomas derived from purified Ly-1 B cells. Definition of the VH11 family. J Immunol 142:3643–3651.
Carmack CE, Shinton SA, Hayakawa K, Hardy RR (1990) Rearrangement and selection of VH11 in the Ly-1 B cell lineage. J Exp Med 172:371–374.
Hayakawa K (1989) Autoreactivity and CD5+B cells. Curr Opin Immunol 2:582–587.
Hayakawa K, Hardy RR, Parks DR, Herzenberg LA (1983) The “Ly-1 B” cell subpopulation in normal immunodefective, and autoimmune mice. J Exp Med 157:202–218.
Hayakawa K, Hardy RR, Honda M, Herzenberg A, Steinberg AD, Herzenberg LA (1984) Ly-1 B cells: functionally distinct lymphocytes that secrete IgM autoantibodies. Proc Natl Acad Sci. U S A 81:2494–2498.
Stall AM, Farinas MC, Tarlinton DM, Lalor A, Herzenberg LA, Strober S, Herzenberg LA (1988) Ly-1 B-cell clones similar to human chronic lymphocytic leukemias routinely develop in older normal mice and young autoimmune (New Zealand Black-related) animals. Proc Natl Acad Sci. U S A 85:7312–7316.
Hayakawa K, Hardy RR (1988) Normal, autoimmune, and malignant CD5+ B cells: the Ly-1 B lineage?. Annu Rev Immunol 6:197–218.
Pennell CA, Mercolino TJ, Grdina TA, Arnold W, Haughton G, Clarke SH (1989) Biased Immunoglobulin variable region gene expression by Ly-1 B cells due to clonal selection. EurJ Immunol 19:1289–1295.
Pennell CA, Arnold LW, Haughton G, Clarke SH (1988) Restricted Ig variable region gene expression among Ly-1+ B cell lymphomas. J Immunol 141:2788–2796.
Hardy RR, Hayakawa K, Shimizu M, Yamasaki K, Kishimoto T (1987) Rheumatoid factor secretion from human Leu-1+ B cells. Science 236:81–83.
Kipps TJ (1989) The CD5 B Cell. Adv. Immunol. 47:117–185.
Miller RA, Gralow J (1984) The induction of Leu-1 antigen expression in human malignant and normal B cells by phorbol myristic acetate (PMA). J Immunol 133:3408–3414.
Cong YZ, Rabin E, Wortis HH (1991) Treatment of murine CD5-B cells with anti-Ig, but not LPS, induces surface CD5: two B-cell activation pathways. Int Immunol 3:467–476.
Feeney AJ (1990) Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences. J Exp Med 172:1377–1390.
Gu H, Forster I, Rajewsky K (1990) Sequence homologies, N sequence insertion and JH gene utilization in VHDJH joining: implications for the joining mechanism and the ontogenetic timing of Ly1 B cell and B-CLL progenitor generation. Embo J 9:2133–2140.
Yancopoulos GD, Malynn BA, Alt FW (1988) Developmentally regulated and strainspecific expression of murine VH gene families. J Exp Med 168:417–435.
Perlmutter RM, Kearney JF, Chang SP, Hood LE (1985) Developmentally controlled expression of immunoglobulin VH genes. Science 227:1597–1601.
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© 1992 Springer-Verlag Berlin Heidelberg
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Hardy, R.R., Shinton, S.A., Hayakawa, K. (1992). Repopulation of SCID Mice with Fetal-Derived B-Lineage Cells. In: Potter, M., Melchers, F. (eds) Mechanisms in B-Cell Neoplasia 1992. Current Topics in Microbiology and Immunology, vol 182. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77633-5_9
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DOI: https://doi.org/10.1007/978-3-642-77633-5_9
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