Mutational Analysis of the Transforming Function of the EBV Encoded LMP-1
Epstein-Barr virus, a member of the herpesvirus group, infects B cells in vitro and in vivo. In vitro the virus infects small resting B cells and drives them to become immortalized, latently infected, proliferating B lymphoblasts. This process has been employed as a model system for studying the mechanisms of herpesvirus latency, B cell activation and oncogenic transformation. To date nine virus encoded proteins have been detected in latently infected B cells (reviewed in Kieff and Liebowitz 1989) consisting of six nuclear proteins (EBNA’s) and three membrane proteins (LMP-1, TP-1 and TP-2). Of these only LMP-1 has been demonstrated to have the function of a transforming oncogene in rodent fibroblasts such as Balb 3T3 (Baichwal and Sugden 1988) and Rat 1 cells (Wang et al 1985).
KeywordsToxicity Codon Glycine Serine Lysine
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