Mutational Analysis of the Transforming Function of the EBV Encoded LMP-1

  • R. Moorthy
  • D. A. Thorley-Lawson
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 182)


Epstein-Barr virus, a member of the herpesvirus group, infects B cells in vitro and in vivo. In vitro the virus infects small resting B cells and drives them to become immortalized, latently infected, proliferating B lymphoblasts. This process has been employed as a model system for studying the mechanisms of herpesvirus latency, B cell activation and oncogenic transformation. To date nine virus encoded proteins have been detected in latently infected B cells (reviewed in Kieff and Liebowitz 1989) consisting of six nuclear proteins (EBNA’s) and three membrane proteins (LMP-1, TP-1 and TP-2). Of these only LMP-1 has been demonstrated to have the function of a transforming oncogene in rodent fibroblasts such as Balb 3T3 (Baichwal and Sugden 1988) and Rat 1 cells (Wang et al 1985).


Methylene Blue Latent Membrane Protein Threonine Residue Serine Phosphorylation Transmembrane Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baichwal VR, Sugden B (1987) Posttranslational processing of an Epstein-Barr virus encoded membrane protein expressed in cells transformed by Epstein-Barr virus. J Virol 61:866–875.PubMedGoogle Scholar
  2. Baichwal VR, Sugden B (1988) Transformation of Balb-3T3 cells by the BNLF-1 gene of Epstein-Barr virus. Oncogene 2:461–468.PubMedGoogle Scholar
  3. Hammerscmidt W Sugden B Baichwal VR (1989) The transforming domain alone of the latent membrane protein of Epstein-Barr virus is toxic to cells when expressed at high levels. J Virol 63:2469–2475.Google Scholar
  4. Henderson S Rowe M Gregory C Croom-Carter D Wang F Longnecker R Kieff E Rickinson A. (1991) Induction of bcl-2 expression by Epstien-Barr virus latent membrane protein protects cells from programmed cell death. Cell 65:1107–1115.PubMedCrossRefGoogle Scholar
  5. Kieff E, Liebowitz D (1989) Epstein-Barr Virus and its Replication In: Virology. Fields B. and D. Knipe, eds. Raven Press, New York, p. 1889–1920.Google Scholar
  6. Mann KP Staunton D Thorley-Lawson DA (1998) An Epstein-Barr virus encoded protein found in the plasma membranes of transformed cells. J Virol 55:710–720.Google Scholar
  7. Mann KP and Thorley-Lawson DA (1987) Posttranslational processing of the Epstein-Barr virus encoded p63/LMP protein. J Virol 61:2100–2108.PubMedGoogle Scholar
  8. Moorthy RK and Thorley-Lawson DA (1990) Processing of the Epstein-Barr virus encoded latent membrane proteinp63/LMP. J Virol 64:829–837.PubMedGoogle Scholar
  9. Wang D, Liebowitz D, Kieff E (1985) An EBV membrane protein expressed in immortalised lymphocytes transform established rodent cells. Cell 43: 831–840.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • R. Moorthy
    • 1
  • D. A. Thorley-Lawson
    • 1
  1. 1.Dept. of PathologyTufts University School of MedicineBostonUSA

Personalised recommendations