Control of Intramarrow B-Cell Genesis by Stromal Cell-Derived Molecules
A remarkable progress has been made in the investigation of B cell-genesis since Whitlock and Witte have reported a long-term culture system of bone marrow B cells (Whitlock and Witte 1982). Although some more stromal cell molecules are yet to be cloned before concluding that we know all molecules involved in the intramarrow B cellgenesis, the basic framework of the molecular requirements for B cell-genesis has been understood to a considerable extent. As a result of this progress, a numbers of methods which use stromal cell lines and recombinant cytokines to control B cell-genesis in vitro are presently available (for review see Kincade et al. 1989; Rolink and Melchers 1991, Kunisada et al. 1992). It is also true, however, that some inconsistency is still present among the previous results on the growth signal requirement of B precursors. Given that control of cell proliferation in higher organisms might be achieved in a redundant and failsafe manner, this redundancy could be a source of the inconsistency among the previous results. Thus, in this article, we would like to re-examine three key propositions which we have been stated in the previous studies and discuss them in detail in light of the previous results of ours and other groups.
KeywordsStromal Cell Normal Bone Marrow Bone Marrow Culture Stromal Cell Line Murine Hematopoietic Stem Cell
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