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Progenitor and Precursor B Lymphocytes of Mice. Proliferation and Differentiation In Vitro and Population, Differentiation and Turnover in SCID Mice In Vivo of Normal and Abnormal Cells

  • F. Melchers
  • D. Haasner
  • H. Karasuyama
  • L. Reininger
  • A. Rolink
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 182)

Abstract

Mouse progenitor B cells (pro B cells*), with H and L chain genes in germline configuration, and precursor B cells with L chain gene loci in germline configuration and H chain gene loci in DHJH-rearranged forms (pre B-I cells, ref. 1) can be grown from single cells of fetal liver, of neonatal liver, spleen, blood and bone marrow, and from adult bone marrow for several months in serum-substituted media on stromal cells in the presence of IL7. When IL7 is removed from the medium, more than 90% of the progenitors and precursors loose the capacity to proliferate on stromal cells in the presence of IL7 within 2 days. In these two days they rearrange their H and L chain gene loci in- and out-of-frame and thereby generate slg- and slg+ B cells which both die rapidly by apoptosis. The slg+ B cells can be activated by lipopolysaccharide (LPS), antigen and IL2 to a primary response of proliferation and maturation to IgM-secreting, plaque forming cells (PFC). Most of these findings have been published by Rolink et al. [7].

Keywords

Stromal Cell Fetal Liver SCID Mouse Chain Locus Plaque Form Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • F. Melchers
    • 1
  • D. Haasner
    • 1
  • H. Karasuyama
    • 1
  • L. Reininger
    • 1
  • A. Rolink
    • 1
  1. 1.Basel Institute for ImmunologyBaselSwitzerland

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