Progenitor and Precursor B Lymphocytes of Mice. Proliferation and Differentiation In Vitro and Population, Differentiation and Turnover in SCID Mice In Vivo of Normal and Abnormal Cells
Mouse progenitor B cells (pro B cells*), with H and L chain genes in germline configuration, and precursor B cells with L chain gene loci in germline configuration and H chain gene loci in DHJH-rearranged forms (pre B-I cells, ref. 1) can be grown from single cells of fetal liver, of neonatal liver, spleen, blood and bone marrow, and from adult bone marrow for several months in serum-substituted media on stromal cells in the presence of IL7. When IL7 is removed from the medium, more than 90% of the progenitors and precursors loose the capacity to proliferate on stromal cells in the presence of IL7 within 2 days. In these two days they rearrange their H and L chain gene loci in- and out-of-frame and thereby generate slg- and slg+ B cells which both die rapidly by apoptosis. The slg+ B cells can be activated by lipopolysaccharide (LPS), antigen and IL2 to a primary response of proliferation and maturation to IgM-secreting, plaque forming cells (PFC). Most of these findings have been published by Rolink et al. .
KeywordsLymphoma Tyrosine Polypeptide Disulfide Integrin
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