Progenitor and Precursor B Lymphocytes of Mice. Proliferation and Differentiation In Vitro and Population, Differentiation and Turnover in SCID Mice In Vivo of Normal and Abnormal Cells
Mouse progenitor B cells (pro B cells*), with H and L chain genes in germline configuration, and precursor B cells with L chain gene loci in germline configuration and H chain gene loci in DHJH-rearranged forms (pre B-I cells, ref. 1) can be grown from single cells of fetal liver, of neonatal liver, spleen, blood and bone marrow, and from adult bone marrow for several months in serum-substituted media on stromal cells in the presence of IL7. When IL7 is removed from the medium, more than 90% of the progenitors and precursors loose the capacity to proliferate on stromal cells in the presence of IL7 within 2 days. In these two days they rearrange their H and L chain gene loci in- and out-of-frame and thereby generate slg- and slg+ B cells which both die rapidly by apoptosis. The slg+ B cells can be activated by lipopolysaccharide (LPS), antigen and IL2 to a primary response of proliferation and maturation to IgM-secreting, plaque forming cells (PFC). Most of these findings have been published by Rolink et al. .
KeywordsStromal Cell Fetal Liver SCID Mouse Chain Locus Plaque Form Cell
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- 8.Melchers F, Strasser A, Bauer SR, Kudo A, Thalmann P, Rolink A (1989) Cellular stages and molecular steps of murine B-cell development. Cold Spring Harbor Symp. Quant. Biol. LIV:183–189.Google Scholar
- 13.McNiece IK, Langley KE, Zsebo KM (1991) The role of recombinant stem cell factor in early B cell development. J. Immunol. 156:3785–3790.Google Scholar
- 18.Takai Y, Sakata T, Iwagami S, Tai G, Kita Y, Hamaoka T, Sakaguchi N, Yamagishi H, Tsuruta T, Teraoka H, Fujiwara H (1992) Identification of IL-7-dependent bone marrow-derived thy-, B220-, lymphoid cell clones that rearrange and express both Ig and T cell receptor genes. J. Immunol. 148:1329–1337.PubMedGoogle Scholar
- 31.Reininger L, Radaszkiewicz T, Kosco M, Melchers F, Rolink A (1992) Development of autoimmune disease in SCID mice populated with long-term “in vitro” proliferating (NZBxNZW) Fl pre B cells. J. Exp. Med., in press.Google Scholar