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Biology of Borrelia burgdorferi

  • V. Preac-Mursic
  • B. Wilske

Abstract

Isolation and in vitro cultivation of Borrelia burgdorferi were first accomplished from Ixodes dammini ticks by Burgdorfer et al. (1982) and from patients by Benach et al. (1983) and Steere et al. (1983) in the Unites States. In Europe, identical microorganisms were detected and isolated from I. ricinus by Burgdorfer et al. (1983) and Barbour et al. (1983a) respectively. Isolates from patients were subsequently accomplished by Ackermann et al. (1984), Asbrink (1985), Asbrink et al. (1984) and Preac-Mursic et al. (1984). An important contribution had earlier been made by Kelly, who, in 1971 described a new medium permitting the cultivation of B. hermsii and other borreliae. Later on, Kelly’s medium was repeatedly modified especially for the cultivation of B. burgdorferi. The ability to grow B. burgdorferi in vitro has greatly facilitated studies concerning its morphological and biological characteristics.

Keywords

Lyme Disease Borrelia Burgdorferi Lyme Borreliosis Linear Plasmid Plasmid Profile 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ackermann R, Kabatzki J, Boisten HP, Steere AC, Grodzicki RL, Härtung S, Runne U (1984) Spirochäten-Ätiologie der Erythema-chronicum-migrans-Krankheit. Dtsch Med Wochenschr 109:92–97PubMedCrossRefGoogle Scholar
  2. Anderson JF, Magnarelli LA, LeFebvre RB, Andreadis TG, McAnich JB, Perng GC, Johnson RC (1989) Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. J Clin Micobiol. 27:13–20Google Scholar
  3. Asbrink E (1985) Erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans. Early and late manifestations of Ixodes ricinus-borne Borrelia spirochetes. Akademisk Avhandling, StockholmGoogle Scholar
  4. Asbrink E, Hederstedt B, Hovmark A (1984) The spirochetal etiology of erythema chronicum migrans Afzelius. Acta Derm Venereol (Stockh) 64:291–295Google Scholar
  5. Barbour AG (1988) Plasmid analysis of Borrelia burgdorferi, the Lyme borreliosis agent. J Clin Microbiol 26:475–478PubMedGoogle Scholar
  6. Barbour AG, Garon CF (1987) Linear plasmids of the bacterium Borrelia burgdorferi have covalently closed ends. Science 237:409–411PubMedCrossRefGoogle Scholar
  7. Barbour AG, Hayes SF (1986) Biology of Borrelia species. Microbiol Ref 4 (50) 381–400Google Scholar
  8. Barbour AG, Schrumpf ME (1986) Polymorphisms of major surface proteins of Borrelia burgdorferi. Zentralbl Bakteriol Hyg (A) 263:83–91Google Scholar
  9. Barbour AG, Burgdorfer W, Hayes SF, Péter O, Aeschlimann A (1983a) Isolation of a cultivable spirochete from Ixodes ricinus ticks of Switzerland. Curr Microbiol 8:123–126CrossRefGoogle Scholar
  10. Barbour AG, Tessier SL, Todd WJ (1983b) Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infect Immun 41:795–804PubMedGoogle Scholar
  11. Barbour AG, Tessier SL, Hayes SF (1984) Variation in a major surface protein of Lyme disease spirochetes. Infect Immun 45:94–100PubMedGoogle Scholar
  12. Barbour AG, Hayes SF, Heiland RA, Schrumpf ME, Tessier SL (1986) A Borrelia-specific monoclonal antibody binds to a flagellar epitope. Infect Immun 52:549–554PubMedGoogle Scholar
  13. Baril C, Richaud C, Baranton G, Saint Girons J (1989) Linear chromosome of Borrelia burgdorferi. Res Microbiol 140:507–516PubMedCrossRefGoogle Scholar
  14. Barthold SW, Moody KD, Terwilliger JA, Duray PH, Jacoby RO, Steere AC (1988) Experimental Lyme arthritis of rats infected with Borrelia burgdorferi. J Infect Dis 157:842–846PubMedCrossRefGoogle Scholar
  15. Beck G, Habicht GS (1988) Isolation and biological activity of Borrelia burgdoiferi pepti-doglycan. Ann NY Acad Sci 539:365–366CrossRefGoogle Scholar
  16. Benach JL, Bosler EM, Hanrahan JP, Coleman JL, Habicht GS, Bost TF, Cameron DJ, Ziegler JL, Barbour AG, Burgdorfer W, Edelman R, Kaslow RA (1983) Spirochetes isolated from the blood of two patients with Lyme disease. N Engl J Med 308:740–742PubMedCrossRefGoogle Scholar
  17. Bundoc VG, Barbour AG (1989) Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdoiferi. Infect Immun 57:2733–2741PubMedGoogle Scholar
  18. Burgdorfer W (1984) The New Zealand white rabbit. An experimental host for infecting ticks with Lyme disease spirochetes. Yale J Biol Med 57:609–612PubMedGoogle Scholar
  19. Burgdorfer W (1991) Lyme borreliosis - a relapsing fever-like illness? Scand J Infect Dis Suppl 77:17–22PubMedGoogle Scholar
  20. Burgdorfer W, Gage KL (1987) Susceptibility of the hispid cotton rat (Sigmodon hispidus) to the Lyme disease spirochete (Borrelia burgdorferi). Am J Trop Med Hyg 37:624–628PubMedGoogle Scholar
  21. Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davis JP (1982) Lyme disease - a tick-borne spirochetosis? Science 216:1317–1319PubMedCrossRefGoogle Scholar
  22. Burgdorfer W, Barbour AG, Hayes SF, Péter O, Aeschlimann A (1983) Erythema chronicum migrans - a tick-borne spirochetosis. Acta Trop (Basel) 40:17–83Google Scholar
  23. Burgess EC (1986) Experimental inoculation of dogs with Borrelia burgdorferi. Zentralbl Bakteriol (A) 263:49–54Google Scholar
  24. Cluss RG, Boothby JT (1990) Thermoregulation of protein synthesis in Borrelia burgdorferi. Infect Immun 58:1038–1042PubMedGoogle Scholar
  25. Coleman JL, Benach JL, Beck G, Habicht GS (1986) Isolation of outer envelope from Borrelia burgdorferi. Zentralbl Bakteriol Hyg (A) 263:123–126Google Scholar
  26. Elwell LP, Shipley PI (1980) Plasmid-mediated factors associated with virulence of bacteria to animals. Annu Rev Microbiol 34:465–496PubMedCrossRefGoogle Scholar
  27. Ferdows MS, Barbour AG (1989) Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent. Proc Natl Acad Sci USA 86:5969–5973PubMedCrossRefGoogle Scholar
  28. Fumarola D, Munno J, Marcuccio C, Miragliotta G (1986) Endotoxicity associated with the Lyme disease Borrelia: recent findings. Microbiologica 9:249–252PubMedGoogle Scholar
  29. Garon CL, Dorward DW, Lubke LL, Corwin MD (1990) Ultrastructural detection of a DNA- containing compartment on the surface of Borrelia burgdorferi cells (poster). In: 4th international conference on Lyme borreliosis, StockholmGoogle Scholar
  30. Gassmann GS, Kramer M, Göbel UB, Wallich R (1989) Nucleotide sequenze of a gene encoding theB. burgdorferi flagellin. Nucleic Acids Res 17:3590PubMedCrossRefGoogle Scholar
  31. Goodman JL, Jurkovich P, Kramber JM, Johnson RC (1991) Molecular detection of persistent Borrelia burgdorferi in the urine of patients with active Lyme disease. Infect Immun 59:269–278PubMedGoogle Scholar
  32. Habicht GS, Beck G, Benach JL, Coleman JL (1986) Borrelia burgdorferi lipopolysaccharide and its role in the pathogenesis of Lyme disease. Zentralbl Bakteriol Hyg (A) 263:137–141Google Scholar
  33. Hansen K, Bangsborg JM, Fjordvang H, Pedersen NS, Hindersson P (1988) Immunochemical characterization and isolation of the gene for a Borrelia burgdorferi immunodominant 60- kilodalton antigen common to a wide range of bacteria. Infect Immun 56:2047–2053PubMedGoogle Scholar
  34. Holt JG, Krieg NR (eds) (1984) Bergey’s manual of systematic bacteriology, vol 1. Williams and Wilkins, BaltimoreGoogle Scholar
  35. Hyde FW, Johnson RC (1984) Genetic relationship of Lyme disease spirochetes toBorrelia, Treponema, andLeptospira spp. J Clin Microbiol 20:151–154PubMedGoogle Scholar
  36. Hyde FW, Johnson RC (1988) Characterization of a circular plasmid from Borrelia burgdorferi etiologic agent of Lyme disease. J Clin Microbiol 26:2203–2205PubMedGoogle Scholar
  37. Johnson RC, Hyde FW, Rumpel CM (1984a) Taxonomy of the Lyme disease spirochetes. Yale J Biol Med 57:79–87Google Scholar
  38. Johnson RC, Schmid GP, Hyde FW, Steigerwalt AG, Brenner DJ (1984b) Borrelia burgdorferi sp.nov: etiologic agent of Lyme disease. Int J Syst Bacteriol 34:496–497CrossRefGoogle Scholar
  39. Johnson RC, Marek N, Kodner C (1984c) Infection of Syrian hamsters with Lyme disease spirochetes. J Clin Microbiol 20:1099–1101PubMedGoogle Scholar
  40. Johnson RC, Kodner C, Russel M, Duray PH (1988) Experimental infection of the hamster with Borrelia burgdorferi. Ann NY Acad Sci 539:258–263PubMedCrossRefGoogle Scholar
  41. Kelly RT (1971) Cultivation of Borrelia hermsii. Science 173:443–444PubMedCrossRefGoogle Scholar
  42. Kelly RT (1976) Cultivation and physiology of relapsing fever borreliae. In: Johnson RC (ed) The biology of parasitic spirochetes. Academic, New York p 87Google Scholar
  43. Kornblatt AN, Steere AC, Brownstein DG (1984) Experimental Lyme disease in rabbits: spirochetes found in erythema migrans and blood. Infect Immun 46:220–223PubMedGoogle Scholar
  44. Kurtti TJ, Munderloh UG, Johnson RC, Ahlstrand GG (1987) Colony formation and morphology in Borrelia burgdorferi. J Clin Microbiol 25:2054–2058PubMedGoogle Scholar
  45. Livermore BP, Bey RF, Johnson RC (1978) Lipid metabolism of Borrelia hermsii. Infect Immun 20:215–220PubMedGoogle Scholar
  46. Persing DH, Telford SR, Spielman A, Barthold SW (1990) Detection of Borrelia burgdorferi infection inIxodes dammini ticks with the polymerase chain reaction. J Clin Microbiol 28:566–572PubMedGoogle Scholar
  47. Pillot Z (1965) Contribution à 1’ étude du genre Treponema: structure anatomique et antige- nique. Thesis, ParisGoogle Scholar
  48. Postic D, Edlinger C, Rauchaud C, Grimont F, Dufresne Y, Perolat P, Baranton G, Grimon PAD (1990) Two genomic species in Borrelia burgdorferi. Res Microbiol 141:465–475PubMedCrossRefGoogle Scholar
  49. Preac-Mursic V, Schierz G, Pfister-HW, Einhäupl K, Wilske B, Weber K (1984) Isolierung einer Spirochäte aus Liquor cerebrospinalis bei Meningoradikulitis Bannwarth. Munch Med Wochenschr 126:275–276Google Scholar
  50. Preac-Mursic V, Wilske B, Schierz G (1986) European Borrelia burgdorferi isolated from humans and ticks: culture conditions and antibiotic susceptibility. Zentralbl Bakteriol Hyg (A) 263:112–118Google Scholar
  51. Preac-Mursic V, Wilske B, Schierz G, Holmburger M, Süß E (1987) In vitro and in vivo susceptibility of Borrelia burgdorferi. Eur J Clin Mibrobiol 6:424–426CrossRefGoogle Scholar
  52. Preac-Mursic V, Patsouris E, Wilske B, Reinhardt S, Groß B (1990) Persistence of Borrelia burgdorferi and histopathological alterations in experimentally infected gerbils. Comparison with histopathological findings in human Lyme disease. Infection 18:1–10CrossRefGoogle Scholar
  53. Preac-Mursic V, Wilske B, Reinhardt S (1991) Culture of Borrelia burgdorferi on six solid media. Eur J Clin Microbiol 10:1076–1079CrossRefGoogle Scholar
  54. Rosa PA, Schwan TG (1991) A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using polymerase chain reaction. J Infect Dis 160:1018–1029CrossRefGoogle Scholar
  55. Rosa PA, Hogan D, Schwan TG (1991) Polymerase chain reaction analyses indentify two distinct classes of Borrelia burgdorferi. J Clin Microbiol 29:524–532PubMedGoogle Scholar
  56. Schaible UE, Kramer MD, Museteanu C, Zimmer G, Mossmann H, Simon MM (1989) The severe combined immunodeficiency (SCID) mouse. A laboratory model for analysis of Lyme arthritis and carditis. J Exp Med 170:1427–1431PubMedCrossRefGoogle Scholar
  57. Schmid GP, Steigerwalt AG, Johnson SE, Barbour AG, Steere AC, Robinson JM, Brenner DJ (1984) DNA characterization of the spirochete that causes Lyme disease. J Clin Microbiol 20:155–158PubMedGoogle Scholar
  58. Schwan TG, Barbour AG (1988) Efficacy of nucleic acid hybridization probes for the detection and identification of Borrelia burgdorferi. Ann NY Acad Sci 539:419–421CrossRefGoogle Scholar
  59. Schwan TG, Burgdorfer W (1987) Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis 156:852–853PubMedCrossRefGoogle Scholar
  60. Schwan TG, Burgdorfer W, Garon CF (1988a) Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation. Infect Immun 56:1831–1836PubMedGoogle Scholar
  61. Schwan TG, Burgdorfer W, Schrumpf ME, Karstens RH (1988b) The urinary bladder, a consistent source of Borrelia burgdorferi in experimentally infected white-footed mice (Peromys- cus leucopus). J Clin Microbiol 26:893–895PubMedGoogle Scholar
  62. Simpson WJ, Burgdorfer W, Schrumpf ME, Karstens RH, Schwan TG (1991) Antibody to a 39- KilodaltonBorrelia burgdorferi antigen (P39) as a marker for infection in experimentally and naturally inoculated animals. J Clin Microbiol 29:236–243PubMedGoogle Scholar
  63. Stanek G, Burger I, Hirschl A, Wewalka G, Radda IA (1986)Borrelia transfer by ticks during their life cycle. Zentralbl Bakteriol Hyg (A) 263:29–33Google Scholar
  64. Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista S (1983) The spirochetal etiology of Lyme disease. N Engl J Med 308:733–740PubMedCrossRefGoogle Scholar
  65. Stoenner HG (1974) Biology of Borrelia hermsii in Kelly medium. Appl Microbiol 28:540–543PubMedGoogle Scholar
  66. Takayama K, Rothenberg RJ, Barbour AG (1987) Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi. Infect Immun 55:2311–2313PubMedGoogle Scholar
  67. Wilske B, Preac-Mursic V, Schierz G (1985) Antigenic heterogeneity of European Borrelia burgdorferi strains isolated from patients and ticks. Lancet I: 1099PubMedCrossRefGoogle Scholar
  68. Wilske B, Preac-Mursic B, Schierz G, von Busch K (1986) Immunochemical and immunological analysis of EuropeanBorrelia burgdorferi strains. Zentral Bakteriol Hyg (A) 263:92–102Google Scholar
  69. Wilske B, Preac-Mursic V, Schierz G, Kühbeck R, Barbour AG, Kramer M (1988) Antigenic variability of Borrelia burgdorferi. Ann NY Acad Sci 539:126–143PubMedCrossRefGoogle Scholar
  70. Wilske B, Preac-Mursic V, Fuchs R, Bruckbauer H, Hofmann A, Zumstein G, Jauris S, Soutschek E, Motz M (1990) Immundominant proteins of Borrelia burgdorferi; implications for improving serodiagnosis of Lyme borreliosis. In: Neu HC (eds) New antibacterial strategies. Churchill Livingstone, LondonGoogle Scholar
  71. Wilske B, Anderson JF, Barbour AG, Hovind-Hougen K, Johnson RC, Preac-Mursic V (1991) Taxonomy of Borrelia spp. Scand J Infect Dis Suppl 77:108–129PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • V. Preac-Mursic
  • B. Wilske

There are no affiliations available

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