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Microbiological Diagnosis of Lyme Borreliosis

  • B. Wilske
  • V. Preac-Mursic

Abstract

Borrelia burgdorferi may be observed microscopically either as living and unstained organisms or after the application of dyes. Because of the small number of bacteria in specimens from humans, especially in blood and cerebrospinal fluid (CSF), organisms are difficult to find using direct microscopy. Centrifugation of specimens improves detection in many cases. Suitable specimens for detection of B. burgdorferi are listed in Table 1. For the direct examination of B. burgdorferi, various methods and preparations are currently used.

Keywords

Lyme Disease Borrelia Burgdorferi Lyme Borreliosis Erythema Migrans Microbiological Diagnosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aberer E, Manitz M, Neumann R, Stanek G (1988) Immunoperoxidase staining of spirochestes in borrelia skin disease. Ann NY Acad Sci 539:362–364Google Scholar
  2. Ackermann R, Kabatzki J, Boisten HP, Steere AC, Grodzicki RL, Hartung S, Runne U (1984) Spirochäten-Ätiologie der Erythema-chronicum-migrans-Krankheit. Dtsch Med Wochenschr 109:92–97PubMedGoogle Scholar
  3. Ackermann R, Gollmer E, Rehse-Küpper B (1985) Progressive Borrelien-Encephalomyelitis. Dtsch Med Wochenschr 26:1039–1042Google Scholar
  4. Aesehlimann A, Chamot E, Gigon F, Jeanneret JP, Kesseler D, Walter C (1986) B. burgdorferi in Switzerland. Zentralbl Bacteriol Hyg (A) 263:450–458Google Scholar
  5. Aesehlimann A, Gern L, Zhiova E, Frossard E, Walter A, Fahrer H, Sauvain MJ, van den Linden S, Gerber N (1988) Observations of two high-risk populations from the Swiss Plateau a region heavily infested with Ixodes ricinus/Borrelia burgdorferi complex. Ann NY Acad Sci 539:440–443Google Scholar
  6. Anderson JF, Magnarelli LA, Lebvre RB, Andreadis TG, Mninch JB, Perng G-C, Johnson RC (1989) Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. J Clin Microbiol 27:13–20PubMedGoogle Scholar
  7. Asbrink E, Hovmark A (1985) Successful cultivation of spirochetes from skin lesion of patients with erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans. Acta Pathol Microbiol Immunol Scand (B) 93:161–163Google Scholar
  8. Asbrink E, Hederstedt B, Hovmark A (1984a) The spirochetal etiology of erythema chronicum migrans Afzelius. Acta Derm Venereol (Stockh) 64:291–295Google Scholar
  9. Asbrink E, Hovmark A, Hederstedt B (1984b) The spirochetal etiology of acrodermatitis chronica atrophicans Herxheimer. Acta Derm Venereol (Stockh) 64:506–512Google Scholar
  10. Asbrink E, Hovmark A, Hederstedt B (1985) Serological studies of erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans with indirect immunofluorescence and enzymelinked immunosorbent assays. Acta Derm Venereol (Stockh) 65:509–514Google Scholar
  11. Asbrink E, Hovmark A, Olsson I (1989) Lymphadenosis benigna cutis solitaria-Borrelia lymphocytoma in Sweden. Zentralbl Bacteriol (Suppl) 18:156–163Google Scholar
  12. Barbour AG, Hayes SF (1986) Biology of Borrelia species. Microbiol Rev 50:381–400PubMedGoogle Scholar
  13. Barbour AG, Burgdorfer W, Grunwald E, Steere AC (1983a) Antibodies of patients with Lyme disease to components of the Ixodes dammini spirochete. J Clin Invest 73:504–515Google Scholar
  14. Barbour AG, Burgdorfer W, Hayes SF, Peter O, Aesehlimann A (1983b) Isolation of a cultivable spirochete from Ixodes ricinus ticks of Switzerland. Curr Microbiol 8:123–126Google Scholar
  15. Barbour AG, Tessier SL, Todd WJ (1983c) Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infect Immun 41:795–804PubMedGoogle Scholar
  16. Barbour AG, Tessier SL, Hayes SF (1984) Variation in a major surface protein of Lyme disease spirochetes. Infect Immun 45:94–100PubMedGoogle Scholar
  17. Barbour AG, Hayes SF, Heiland RA, Schrumpf ME, Tessier SL (1986) A Borrelia-specific monoclonal antibody binds to a flagellar epitope. Infect Immun 52:549–554PubMedGoogle Scholar
  18. Benach JL, Bosler EM, Hanrahan JP, Coleman JL, Habicht GS, Bast TF, Cameron DJ, Ziegler JL, Barbour AG, Burgdorfer W, Edelman R, Kaslow RA (1983) Spirochetes isolated from the blood of two patients with Lyme disease. N Engl J Med 308:740–742PubMedGoogle Scholar
  19. Berardi VP, Weeks KE, Steere AC (1988) Serodiagnosis of early Lyme disease: analysis of I and I antibody responses bv using an antibody-capture enzyme immunoassay. J Infect Dis 158:754–760PubMedGoogle Scholar
  20. Berger BW, Kaplan MH, Rothenberg IR, Barbour AG (1985) Isolation and characterization of the Lyme disease spirochete from the skin of patients with erythema chronicum migrans. J Am Acad Dermatol 13:444–449PubMedGoogle Scholar
  21. Bergström S, Bundoc VG, Barbour AG (1989) Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi Mol Microbiol 3:479–486PubMedGoogle Scholar
  22. Bissett ML, Hill W (1987) Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California. J Clin Microbiol 25:2296–2301PubMedGoogle Scholar
  23. Bozsik BP (1985) Borrelia burgdorferi stained with acidic acridine orange. Ann Immunol Hung 25:335–344Google Scholar
  24. Brandt ME, Riley BS, Radolf JD, Norgard MV (1990) Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infect Immun 58:989–991Google Scholar
  25. Bundoc VG, Barbour A (1989) Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdorferi Infect Immun 57:2733–2741PubMedGoogle Scholar
  26. Burgdorfer W, Gage KL (1986) Susceptibility of the black-legged tick, Ixodes scapularis, to the Lyme disease spirochete,Borrelia burgdorferi Zentralbl Bakteriol Hyg (A) 263:15–20Google Scholar
  27. Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwald E, Davis JP (1982) Lyme disease - a tickborne spirochetosis? Science 216:1317–1319PubMedGoogle Scholar
  28. Burgdorfer W, Lane RS, Barbour AG, Gresbrink RA, Anderson JR (1985) The Western black- legged tick, Ixodes pacificus: a vektor of Borrelia burgdorferi Am J Trop Med Hyg 34:925–930PubMedGoogle Scholar
  29. Cerny EH, Farsy CE, Hunter EF, Larsen SA (1985) Rheumatoid factor in syphilis. J Clin Microbiol 22:89–94PubMedGoogle Scholar
  30. Cluss RG, Boothby JT (1990) Thermoregulation of protein synthesis in Borrelia burgdorferi Infect Immun 58:1038–1042Google Scholar
  31. Coleman JL, Benach JL (1987) Isolation of antigenic components from the Lyme disease spirochete: Their role in early diagnosis. J Infect Dis 155:756–765PubMedGoogle Scholar
  32. Craft JE, Grodzicki RL, Steere AC (1984) Antibody response in Lyme disease: evaluation of diagnostic tests. J Infect Dis 149:789–795PubMedGoogle Scholar
  33. Craft JE, Fischer DH, Shumamoto GT, Steere AC (1986) Antigens ofBorrelia burgdorferi recognized during Lyme disease. J Clin Invest 78:934–939PubMedGoogle Scholar
  34. Fuchs R, Wilske B, Preac-Mursic V, Schierz G, Gueye W, Liegl G (1989) The use of purified immunodominant antigens of Borrelia burgdorferi in IgM- and IgG-ELISA (poster). In: European Society of Clinical Microbiology and Infectious Diseases (ed) 4th European congress of clinical microbiology. Nizza, pp 912/PP43Google Scholar
  35. Fuchs R, Wilske B, Preac-Mursic V, Schierz G (1990a) Purification of B. burgdorferi flagellum by use of a monoclonal antibody. Mol Cell Probes 4:451–462PubMedGoogle Scholar
  36. Fuchs R, Soutschek E, Wilske B, Preac-Mursic V (1990b) Identification and molecular characterisation of an immunogenic protein (p100) of Borrelia burgdorferi (abstract) in: 4th international conference on Lyme borreliosis, StockholmGoogle Scholar
  37. Gaßmann GS, Kramer M, Göbel UB, Wallich R (1989) Nucleotide sequence of a gene encoding the B. burgdorferi flagellin. Nucleic Acids Res 17:3590PubMedGoogle Scholar
  38. Goodman JL, Jurkovich P, Kramber JM, Johnson RC (1991) Molecular detection of persistant Borrelia burgdorferi in the urine of patients with active Lyme disease. Infect Immun 59:269–278PubMedGoogle Scholar
  39. Grodzicki RL, Steere AC (1988) Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. J Infect Dis 157:790–797PubMedGoogle Scholar
  40. Hansen K, Asbrink E (1989) Serodiagnosis of erythema migrans and acrodermatitis chronica atrophicans by the Borrelia burgdorferi flagellum enzyme-linked immunosorbent assay. J Clin Microbiol 27:545–551PubMedGoogle Scholar
  41. Hansen K, Bangsborg JM, Fjordvang H, Pedersen NS, Hindersson P (1988a) Immunochemical characterization and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. Infect Immun 56:2047–2053PubMedGoogle Scholar
  42. Hansen K, Hindersson P, Pedersen NS (1988b) Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease. J Clin Microbiol 26:338–346PubMedGoogle Scholar
  43. Hansen K, Cruz M, Link H (1990) Oligoclonal Borrelia burgdorferi-specific IgG antibodies in cerebrospinal fluid in Lyme neuroborreliosis. J Infect Dis 161:1194–1202PubMedGoogle Scholar
  44. Hansen K, Pii K, Lebech A-M (1991) Improved immunoglobulin M serodiagnosis in Lyme borreliosis by using a µ-capture enzyme-linked immunosorbent assay with biotinylated Borrelia burgdorferi flagella. J Clin Microbiol 291:166–173Google Scholar
  45. Hechemy KE, Harris HL, Duerr MJ, Benach JL, Reimer CB (1988) Immunoglobulin G subclasses specific to Borrelia burgdorferi in patients with Lyme disease. Ann NY Acad Sci 539:162–169PubMedGoogle Scholar
  46. Herzer P, Wilske B (1986) Lyme arthritis in Germany. Zentralbl Bakteriol Hyg (A) 263:268–274Google Scholar
  47. Herzer P, Wilske B, Preac-Mursic V, Schierz G, Schattenkirchner M, Zöllner N (1986) Lyme arthritis: clinical features, serological and radiographic findings of cases in Germany. Klin Wochenschr 64:206–215PubMedGoogle Scholar
  48. Herzer P, Wilske B, Schewe S, Preac-Mursic V, Schierz G (1989) Serologische Diagnostik der Lyme-Arthritis. Aktuel Rheumatol 14:125–129Google Scholar
  49. Hofstad H, Matre R, Nyland H, Ulvestad E (1987) Bannwarth’s syndrome: serum and CSF IgG antibodies against Borrelia burgdorferi examined by ELISA. Acta Neurol Scand 75:37–45PubMedGoogle Scholar
  50. Hyde FW, Johnson RC, White TJ, Shelburne CE (1989) Detection of antigens in urine of mice and humans infected with Borrelia burgdorferi, etiologic agent of Lyme disease. J Clin Microbiol 27:58–61PubMedGoogle Scholar
  51. Johnson SE, Klein GC, Sehmid GP, Bowen GS, Feeley JC, Schulze T (1984) Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete. J Clin Microbiol 19:81–82PubMedGoogle Scholar
  52. Jonsson M, Bergström S (1990) Molecular characteristics and genetic organization of the osp operon from different European Borrelia burgdorferi strains. 4th international conference on Lyme borreliosis, StockholmGoogle Scholar
  53. Karlsson M (1990a) Western immunoblot and flagellum enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis. J Clin Microbiol 28:2148–2150PubMedGoogle Scholar
  54. Karlsson M, Hovind-Hougen K, Svenungsson B, Stiernstedt G (1990b) Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis. J Clin Microbiol 28:473–479PubMedGoogle Scholar
  55. Kmety E, Rehacek J, Vyrostekova V (1986) Investigation of ticks for the presence of Borrelia in Czechoslovakia. Zentralbl Bakteriol Hyg (A) 263:468–470Google Scholar
  56. Kristoferitsch W, Sterck AJ, Murray N, Stanek G, Lanschützer H (1986) Oligoclonal antibodies in CSF of patients with meningopolyneuritis Garin-Bujadoux-Bannwarth: Ig class, light chain type and specifity. Zentralbl Bakteriol Hyg (A) 263:307–313Google Scholar
  57. LeFebvre RB, Perng G-C, Johnson RC (1990) The 83-kilodalton antigen of Borrelia burgdorferi which stimulates immunoglobulin M (IgM) and IgG responses in infected hosts is expressed by a chromosomal gene. J Clin Microbiol 28:1673–1676PubMedGoogle Scholar
  58. Link H, Baig S, Olsson T, Zachau A, Stiernstedt G (1988) B-cell response at a cellular level in cerebrospinal fluid and blood in Lyme disease and controls. Ann NY Acad Sci 539:389–392Google Scholar
  59. Luft BJ, Jiang W, Munoz P, Dattwyler RJ, Gorevic PD (1989) Biochemical and immunological characterization of the surface proteins of Borrelia burgdorferi Infect Immun 57:3637–3645PubMedGoogle Scholar
  60. Luther B, Moskophidis M (1990) Antigenic cross-reactivity between Borrelia burgdorferi, Borellia recurrentis, Treponema pallidum and Treponema phagedenis Zentralbl Bakteriol 274:214–226PubMedGoogle Scholar
  61. MacDonald AB (1986) Human fetal borreliosis, toxemia of pregnancy, and fetal death. Zentralbl Bakteriol Hyg (A) 263:189–200Google Scholar
  62. Magnarelli LA (1988) Serological diagnosis of Lyme disease. Ann NY Acad Sci 539:154–161PubMedGoogle Scholar
  63. Magnarelli LA, Anderson JF (1988) Enzyme-linked immunosorbent assays for detection of class-specific immunoglobulins to Borrelia burgdorferi Am J Epidemiol 127:818–825PubMedGoogle Scholar
  64. Magnarelli LA, Meegan JM, Anderson JF, Chappell WA (1984) Comparison of an indirect fluorescent antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease. J Clin Microbiol 20:181–184PubMedGoogle Scholar
  65. Magnarelli LA, Anderson JF, Johnson RC (1987) Cross-reactivity in serological tests for Lyme disease and other spirochetal infections. J Infect Dis 156:183–188PubMedGoogle Scholar
  66. Martin R, Martens U, Krüger H, Sticht-Groh V, Dörries R (1987) Nachweis und Identifizierung Borrelia-burgdorferi-spezifischer, oligoklonarer Immunglobuline (IgG) im unkonzentrierten Liquor mit der Immunoblottechnik. In: Peock K, Hacke W, Schneider R, (eds) Neuroimmunologie, spinale Krankheiten, Neuropsychologic, metabolische Enzephalopathien, neurologische Notfälle, interventionelle Neuroradiologie. Springer, Berlin Heidelberg New York, pp 440–442 (Verhandlungen der Deutschen Gesellschaft für Neurologie, vol 4)Google Scholar
  67. Martin R, Martens U, Sticht-Groh V, Dörries R, Krüger H (1988) Persistent intrathecal secretion of oligoclonal, Borrelia burgdorferi-specific IgG in chronic meningoradiculomyelitis. J Neurol 225:229–239Google Scholar
  68. Müller F (1982) Der 19S-IgM-FTA-Abs-Test in der Serodiagnostik der Syphilis. Technik, Fehlermöglichkeiten und diagnostische Aussagen. Immun Infekt 10:32–34Google Scholar
  69. Müller F, Moskophidis M (1983) Estimation of the local production of antibodies to Treponema pallidum in the central nervous system of patients with neurosyphilis. Br J Vener Dis 59:80–84PubMedGoogle Scholar
  70. Neubert U, Krampitz HE, Engl H (1986) Microbiological findings in erythema (chronicum) migrans and related disorders. Zentralbl Bakteriol Hyg (A) 263:237–252Google Scholar
  71. Park HK, Jones BE, Barbour AG (1986) Erythema chronicum migrans of Lyme disease: diagnosis by monoclonal antibodies. J Am Acad Dermatol 15:406–410PubMedGoogle Scholar
  72. Pfister HW, Neubert U, Wilske B, Preac-Mursic V, Einhäupl KM, Borasio GD (1986) Reinfection with Borrelia burgdorferi Lancet II:984–985Google Scholar
  73. Preac-Mursic V, Schierz G, Pfister H-W, Einhäupl K, Wilske B, Weber K (1984a) Isolierung einer Spirochäte aus Liquor cerebrospinalis bei Meningoradiculitis-Bannwarth. Munch Med Wochenschr 126:275–276Google Scholar
  74. Preac-Mursic V, Wilske B, Schierz G, Pfister H-W, Einhäupl K (1984b) Repeated isolation of spirochetes from the cerebrospinal fluid of a patient with meningoradiculitis Bannwarth. Eur J Clin Microbiol 3:564–565PubMedGoogle Scholar
  75. Preac-Mursic V, Wilske B, Herzer P, Schierz G, Bauer H (1985) Acrodermatitis chronica atrophicans: eine Borreliose! Hautarzt 36:691–693PubMedGoogle Scholar
  76. Preac-Mursic V, Wilske B, Schierz G (1986) European Borrelia burgdorferi isolated from humans and ticks: Culture conditions and antibiotic susceptibility. Zentralbl Bakteriol Hyg (A) 263:112–118Google Scholar
  77. Preac-Mursic V, Weber K, Pfister HW, Wilske B, Gross B, Baumann A, Prokop J (1989) Survival ofBorrelia burgdorferi in antibiotically treated patients with Lyme borreliosis. Infection 17:355–359PubMedGoogle Scholar
  78. Preac-Mursic V, Patsouris E, Wilske B, Reinhardt S, Groß B, Mehrain P (1990) Persistence of Borrelia burgdorferi and histopathological alterations in experimentally infected animals; comparison with histopathological findings in human Lyme disease. Infection 18:332–341PubMedGoogle Scholar
  79. Rehse-Küpper B, Ackermann R (1986) Demonstration of locally synthesized borrelia antibodies in cerebrospinal fluid. Zentralbl Bakteriol Hyg (A) 263:407–411Google Scholar
  80. Reimers CD, Pongratz DE, Neubert U, Pilz A, Hübner G, Naegele M, Wilske B, Duray PH, de Koning JC (1989) Myositis caused by B. burgdorferi: report of four cases. J Neuorol Sci 91:215–226Google Scholar
  81. Rosa PA, Schwan TG (1989) A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. J Infect Dis 160:1018–1029PubMedGoogle Scholar
  82. Röther E, Vogt A, Meyer-Düttingdorf E, Peter HH (1988) Wertigkeit der Serodiagnostik für Verlauf und Therapie der Borreliose. Immun Infekt 16:27–28PubMedGoogle Scholar
  83. Russel H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B (1984) Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis 149:465–470Google Scholar
  84. Schmidli J, Hunziker T, Moesli P, Schaad UB (1988) Cultivation of Borrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis. J Infect Dis 158:905–906PubMedGoogle Scholar
  85. Schwan TG, Burgdorfer W (1987) Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis 156:852–853PubMedGoogle Scholar
  86. Schwan TG, Burgdorfer W, Garon CF (1988) Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of cultivation. Infect Immun 56:1831–1836PubMedGoogle Scholar
  87. Schwan TG, Simpson WJ, Schrumpf ME, Karstens RH (1989) Identification of Borrelia burgdorferi andB. hermsii using DNA hybridization probes. J Clin Microbiol 27:1734–1738PubMedGoogle Scholar
  88. Simpson WJ, Garon CF, Schwan TG (1990a) Borrelia burgdorferi contains DNA sequences that are species specific and plasmid associated. Infect Immun 58:847–853PubMedGoogle Scholar
  89. Simpson WJ, Schrumpf ME, Schwan TG (1990b) Reactivity of human Lyme borreliosis sera with a 39-kilodalton antigen specific to Borrelia burgdorferi Clin Microbiol 28:1329–1337Google Scholar
  90. Simpson WJ, Burgdorfer W, Schrumpf ME, Karstens RH, Schwan TG (1991) Antibody to a 39-kilodalton Borrelia burgdorferi antigen (P39) as a marker for infection in experimentally and naturally inoculated animals. J Clin Microbiol 29:236–243PubMedGoogle Scholar
  91. Stanek G, Wewalka G, Groh V, Neumann R (1985) Isolation of spirochetes from the skin of patients with erythema chronicum migrans in Austria. Zentralbl Bakteriol Hyg (A) 260:88–90Google Scholar
  92. Stanek G, Hirschl A, Kristoferitsch W (1986) EFA and ELISA in serodiagnosis of Lyme borreliosis. Mitt Österr Ges Trop Parasitol 8:1–6Google Scholar
  93. Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE (1983) The spirochetal etiology of Lyme disease. N Engl J Med 308:733–740PubMedGoogle Scholar
  94. Stiernstedt G, Sköldenberg B, Vandvik B, Hederstedt B, Gårde A, Kolmodin G, Jörbäck H, Svenungsson B (1984) Chronic meningitis and Lyme disease in Sweden. Yale J Biol Med 57:491–497PubMedGoogle Scholar
  95. Stiernstedt G, Granström M, Hederstedt B, Sköldenberg B (1985) Diagnosis of spirochetal meningitis by enzyme-linked immunosorbent assay and indirect immunofluorescent assay in serum and cerebrospinal fluid. J Clin Microbiol 21:819–825PubMedGoogle Scholar
  96. Stiernstedt G, Eriksson G, Enfors W, Jörbeck H, Svenungson B, Sköldenberg B, Granström M (1986) Erythema chronicum migrans in Sweden: clinical manifestations and antibodies to Ixodes ricinus spirochete measured by indirect immunofluorescence and enzyme-linked immunosorbent assay. Scand J Infect Dis 18:217–224PubMedGoogle Scholar
  97. Wallich R, Schaible UE, Simon MM, Heiberger A, Kramer MD (1989) Cloning and sequencing of the gene encoding the outer surface protein A (OspA) of a European Borrelia burgdorferi isolate. Nucleic Acids Res 17:8864PubMedGoogle Scholar
  98. Wallich R, Moter SE, Simon MM, Ebnet K, Heiberger A, Kramer MD (1990) The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): Molecular cloning, expression, and amplification of the gene. Infect Immun 58:1711–1719PubMedGoogle Scholar
  99. Wassmann K, Borg-von Zepelin M, Zimmermann O, Stadler M, Eiffert H, Thomssen R (1989) Determination of immunoglobulin M antibody against Borrelia burgdorferi to differentiate between acute and past infections. Zentralbl Bacteriol (Suppl) 18:281–289Google Scholar
  100. Weber K, Schierz G, Wilske B, Preac-Mursic V (1984a) Zur Klinik und Ätiologie der Acrodermatitis chronica atrophicans. Hautarzt 35:571–577PubMedGoogle Scholar
  101. Weber K, Schierz G, Wilske B, Preac-Mursic V (1984b) European erythema migrans disease and related disorders. Yale J Biol Med 57:463–471PubMedGoogle Scholar
  102. Weber K, Schierz G, Wilske B, Preac-Mursic V (1985) Das Lymphozytom - eine Borreliose? Z Hautkr 60:1585–1598PubMedGoogle Scholar
  103. Weber K, Schierz G, Wilske B, Neubert U, Krampitz HE, Barbour AG, Burgdorfer W (1986) Reinfection in erythema migrans disease. Infection 14:32–35PubMedGoogle Scholar
  104. Weber K, Preac-Mursic V, Neubert U, Thurmayr R, Herzer P, Wilske B, Schierz G, Marget W (1988a) Antibiotic therapy of early European Lyme borreliosis and acrodermatitis chronica atrophicans. Ann NY Acad Sci 539:324–345PubMedGoogle Scholar
  105. Weber K, Preac-Mursic V, Reimers CD (1988b) Spirochetes isolated from two patients with morphea. Infection 16:25–26PubMedGoogle Scholar
  106. Wilske B, Schierz G, Preac-Mursic V, Weber K, Pfister H-W, Einhäupl K (1984) Serological diagnosis of erythema migrans disease and related disorders. Infection 5:331–337Google Scholar
  107. Wilske B, Münchhoff P, Schierz G, Preac-Mursic V, Roggendorf M, Zoulek G (1985) Zur Epidemiologie der Borrelia-burgdorferi-lnfektion bei Waldarbeitern aus Oberbayern. Munch Med Wochenschr 127:171–172Google Scholar
  108. Wilske B, Preac-Mursic V, Schierz G, von Busch K (1986a) Immunochemical and immunological analysis of European Borrelia burgdorferi strains. Zentralbl Bakteriol Hyg (A) 263:92–102Google Scholar
  109. Wilske B, Schierz G, Preac-Mursic V, von Busch K, Kühbeck R, Pfister HW, Einhäupl K (1986b) Intrathecal production of specific antibodies against Borrelia burgdorferi in patients with lymphocytic meningoradiculitis (Bannwarth’s syndrome). J Infect Dis 153:304–314PubMedGoogle Scholar
  110. Wilske B, Schierz G, Preac-Mursic V, Bader L, Pfister HW, Einhäupl K, Herzer P (1987) Intrathekale Produktion spezifischer Antikörper gegen Borrelia burgdorferi bei Patienten mit Bannwarth-Syndrome. In: Poeck K, Hacke W, Schneider R (eds) Neuroimmunologie, spinale Krankheiten, Neuropsychologic, metabolische Enzephalopathien, neurologische Notfälle, interventionelle Neuroradiologie. Springer, Berlin Heidelberg New York, pp 443–447 (Verhandlungen der Deutschen Gesellschaft für Neurologie, vol 4)Google Scholar
  111. Wilske B, Preac-Mursic V, Schierz G, Gueye W, Herzer P, Weber K (1988a) Immunochemische Analyse der Immunantwort bei Spätmanifestationen der Lyme Borreliose. (Immunochemical analysis of the immune response in late manifestations of Lyme borreliosis) Zentralbl Bakteriol Hyg (A) 267:549–558Google Scholar
  112. Wilske B, Preac-Mursic V, Schierz G, Kühbeck R, Barbour AG, Kramer M (1988b) Antigenic variability of Borrelia burgdorferi Ann NY Acad Sci 539:126–143PubMedGoogle Scholar
  113. Wilske B, Fuchs R, Schierz G, Preac-Mursic V, Liegl G (1989a) Immunodominant proteins for the humoral immune response in Lyme borreliosis (poster). In: European Society of Clinical Microbiology and Infectious Diseases (ed) 4th European congress of clinical microbiology. Nizza, pp 911/PP43Google Scholar
  114. Wilske B, Preac-Mursic V, Schierz G, Liegl G, Gueye W (1989b) Detection of IgM- and IgG antibodies to Borrelia burgdorferi using different strains as antigen. Zentralbl Bakteriol (Suppl) 18:299–309Google Scholar
  115. Wilske B, Schierz G, Preac-Mursic V, Pfister HW, Weber K, von Busch K, Baruschke A (1989c) IgM- and IgG immune response to Borrelia burgdorferi in erythema migrans and neuroborreliosis. Zentralbl Bakteriol (Suppl) 18:290–298Google Scholar
  116. Wilske B, Preac-Mursic V, Fuchs R, Bruckbauer H, Hofmann A, Zumstein G, Jauris S, Soutschek E, Motz M (1990) Immundominant proteins of Borrelia burgdorferi; implications for improving serodiagnosis of Lyme borreliosis. In: Neu HC (ed) New antibacterial strategies. Churchill Livingstone, LondonGoogle Scholar
  117. Wilske B, Anderson JF, Baranton G, Barbour AG, Hoving-Hougen K, Johnson RC, Preac-Mursic V (1991) Workshop summary: taxonomy of Borrelia spp. Scand J Infect Dis Suppl 77:108–129PubMedGoogle Scholar
  118. Wilske B, Barbour AG, Bergström S, Burman N, Restrepo BI, Rosa PA, Schwan T, Soutschek E, Wallich R (1992) Antigenic variation and strain heterogeneity in Borrelia spp. Res Microbiol 143:in pressGoogle Scholar
  119. Ziegelmeier R, Bieker R, Behrens F, Vermeer H (1983) Diagnostik akuter Infektionen: IgM-Antikörperbestimmung ohne Rheumafaktor-Interferenz. Laboratoriumsblätter 33:19–25Google Scholar
  120. Zöller L, Burkard S, Schäfer H (1991) Validity of Western immunoblot band patterns in the serodiagnosis of Lyme borreliosis. J Clin Microbiol 29:174–182PubMedGoogle Scholar
  121. Zumstein G, Fuchs R, Preac-Mursic V, Wilske B, Soutschek E (1990) Identification and characterisation by restriction endonucleases of the outer surface protein OspA of Borrelia burgdorferi (Poster). In: 4th international conference on Lyme Borreliosis, StockholmGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • B. Wilske
  • V. Preac-Mursic

There are no affiliations available

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