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Microbiological Diagnosis of Lyme Borreliosis

  • Chapter
Aspects of Lyme Borreliosis

Abstract

Borrelia burgdorferi may be observed microscopically either as living and unstained organisms or after the application of dyes. Because of the small number of bacteria in specimens from humans, especially in blood and cerebrospinal fluid (CSF), organisms are difficult to find using direct microscopy. Centrifugation of specimens improves detection in many cases. Suitable specimens for detection of B. burgdorferi are listed in Table 1. For the direct examination of B. burgdorferi, various methods and preparations are currently used.

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References

  • Aberer E, Manitz M, Neumann R, Stanek G (1988) Immunoperoxidase staining of spirochestes in borrelia skin disease. Ann NY Acad Sci 539:362–364

    Google Scholar 

  • Ackermann R, Kabatzki J, Boisten HP, Steere AC, Grodzicki RL, Hartung S, Runne U (1984) Spirochäten-Ätiologie der Erythema-chronicum-migrans-Krankheit. Dtsch Med Wochenschr 109:92–97

    PubMed  CAS  Google Scholar 

  • Ackermann R, Gollmer E, Rehse-Küpper B (1985) Progressive Borrelien-Encephalomyelitis. Dtsch Med Wochenschr 26:1039–1042

    Google Scholar 

  • Aesehlimann A, Chamot E, Gigon F, Jeanneret JP, Kesseler D, Walter C (1986) B. burgdorferi in Switzerland. Zentralbl Bacteriol Hyg (A) 263:450–458

    Google Scholar 

  • Aesehlimann A, Gern L, Zhiova E, Frossard E, Walter A, Fahrer H, Sauvain MJ, van den Linden S, Gerber N (1988) Observations of two high-risk populations from the Swiss Plateau a region heavily infested with Ixodes ricinus/Borrelia burgdorferi complex. Ann NY Acad Sci 539:440–443

    Google Scholar 

  • Anderson JF, Magnarelli LA, Lebvre RB, Andreadis TG, Mninch JB, Perng G-C, Johnson RC (1989) Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. J Clin Microbiol 27:13–20

    PubMed  CAS  Google Scholar 

  • Asbrink E, Hovmark A (1985) Successful cultivation of spirochetes from skin lesion of patients with erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans. Acta Pathol Microbiol Immunol Scand (B) 93:161–163

    CAS  Google Scholar 

  • Asbrink E, Hederstedt B, Hovmark A (1984a) The spirochetal etiology of erythema chronicum migrans Afzelius. Acta Derm Venereol (Stockh) 64:291–295

    CAS  Google Scholar 

  • Asbrink E, Hovmark A, Hederstedt B (1984b) The spirochetal etiology of acrodermatitis chronica atrophicans Herxheimer. Acta Derm Venereol (Stockh) 64:506–512

    CAS  Google Scholar 

  • Asbrink E, Hovmark A, Hederstedt B (1985) Serological studies of erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans with indirect immunofluorescence and enzymelinked immunosorbent assays. Acta Derm Venereol (Stockh) 65:509–514

    CAS  Google Scholar 

  • Asbrink E, Hovmark A, Olsson I (1989) Lymphadenosis benigna cutis solitaria-Borrelia lymphocytoma in Sweden. Zentralbl Bacteriol (Suppl) 18:156–163

    Google Scholar 

  • Barbour AG, Hayes SF (1986) Biology of Borrelia species. Microbiol Rev 50:381–400

    PubMed  CAS  Google Scholar 

  • Barbour AG, Burgdorfer W, Grunwald E, Steere AC (1983a) Antibodies of patients with Lyme disease to components of the Ixodes dammini spirochete. J Clin Invest 73:504–515

    Google Scholar 

  • Barbour AG, Burgdorfer W, Hayes SF, Peter O, Aesehlimann A (1983b) Isolation of a cultivable spirochete from Ixodes ricinus ticks of Switzerland. Curr Microbiol 8:123–126

    Google Scholar 

  • Barbour AG, Tessier SL, Todd WJ (1983c) Lyme disease spirochetes and ixodid tick spirochetes share a common surface antigenic determinant defined by a monoclonal antibody. Infect Immun 41:795–804

    PubMed  CAS  Google Scholar 

  • Barbour AG, Tessier SL, Hayes SF (1984) Variation in a major surface protein of Lyme disease spirochetes. Infect Immun 45:94–100

    PubMed  CAS  Google Scholar 

  • Barbour AG, Hayes SF, Heiland RA, Schrumpf ME, Tessier SL (1986) A Borrelia-specific monoclonal antibody binds to a flagellar epitope. Infect Immun 52:549–554

    PubMed  CAS  Google Scholar 

  • Benach JL, Bosler EM, Hanrahan JP, Coleman JL, Habicht GS, Bast TF, Cameron DJ, Ziegler JL, Barbour AG, Burgdorfer W, Edelman R, Kaslow RA (1983) Spirochetes isolated from the blood of two patients with Lyme disease. N Engl J Med 308:740–742

    PubMed  CAS  Google Scholar 

  • Berardi VP, Weeks KE, Steere AC (1988) Serodiagnosis of early Lyme disease: analysis of I and I antibody responses bv using an antibody-capture enzyme immunoassay. J Infect Dis 158:754–760

    PubMed  CAS  Google Scholar 

  • Berger BW, Kaplan MH, Rothenberg IR, Barbour AG (1985) Isolation and characterization of the Lyme disease spirochete from the skin of patients with erythema chronicum migrans. J Am Acad Dermatol 13:444–449

    PubMed  CAS  Google Scholar 

  • Bergström S, Bundoc VG, Barbour AG (1989) Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi Mol Microbiol 3:479–486

    PubMed  Google Scholar 

  • Bissett ML, Hill W (1987) Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California. J Clin Microbiol 25:2296–2301

    PubMed  CAS  Google Scholar 

  • Bozsik BP (1985) Borrelia burgdorferi stained with acidic acridine orange. Ann Immunol Hung 25:335–344

    Google Scholar 

  • Brandt ME, Riley BS, Radolf JD, Norgard MV (1990) Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infect Immun 58:989–991

    Google Scholar 

  • Bundoc VG, Barbour A (1989) Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdorferi Infect Immun 57:2733–2741

    PubMed  CAS  Google Scholar 

  • Burgdorfer W, Gage KL (1986) Susceptibility of the black-legged tick, Ixodes scapularis, to the Lyme disease spirochete,Borrelia burgdorferi Zentralbl Bakteriol Hyg (A) 263:15–20

    CAS  Google Scholar 

  • Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwald E, Davis JP (1982) Lyme disease - a tickborne spirochetosis? Science 216:1317–1319

    PubMed  CAS  Google Scholar 

  • Burgdorfer W, Lane RS, Barbour AG, Gresbrink RA, Anderson JR (1985) The Western black- legged tick, Ixodes pacificus: a vektor of Borrelia burgdorferi Am J Trop Med Hyg 34:925–930

    PubMed  CAS  Google Scholar 

  • Cerny EH, Farsy CE, Hunter EF, Larsen SA (1985) Rheumatoid factor in syphilis. J Clin Microbiol 22:89–94

    PubMed  CAS  Google Scholar 

  • Cluss RG, Boothby JT (1990) Thermoregulation of protein synthesis in Borrelia burgdorferi Infect Immun 58:1038–1042

    CAS  Google Scholar 

  • Coleman JL, Benach JL (1987) Isolation of antigenic components from the Lyme disease spirochete: Their role in early diagnosis. J Infect Dis 155:756–765

    PubMed  CAS  Google Scholar 

  • Craft JE, Grodzicki RL, Steere AC (1984) Antibody response in Lyme disease: evaluation of diagnostic tests. J Infect Dis 149:789–795

    PubMed  CAS  Google Scholar 

  • Craft JE, Fischer DH, Shumamoto GT, Steere AC (1986) Antigens ofBorrelia burgdorferi recognized during Lyme disease. J Clin Invest 78:934–939

    PubMed  CAS  Google Scholar 

  • Fuchs R, Wilske B, Preac-Mursic V, Schierz G, Gueye W, Liegl G (1989) The use of purified immunodominant antigens of Borrelia burgdorferi in IgM- and IgG-ELISA (poster). In: European Society of Clinical Microbiology and Infectious Diseases (ed) 4th European congress of clinical microbiology. Nizza, pp 912/PP43

    Google Scholar 

  • Fuchs R, Wilske B, Preac-Mursic V, Schierz G (1990a) Purification of B. burgdorferi flagellum by use of a monoclonal antibody. Mol Cell Probes 4:451–462

    PubMed  CAS  Google Scholar 

  • Fuchs R, Soutschek E, Wilske B, Preac-Mursic V (1990b) Identification and molecular characterisation of an immunogenic protein (p100) of Borrelia burgdorferi (abstract) in: 4th international conference on Lyme borreliosis, Stockholm

    Google Scholar 

  • Gaßmann GS, Kramer M, Göbel UB, Wallich R (1989) Nucleotide sequence of a gene encoding the B. burgdorferi flagellin. Nucleic Acids Res 17:3590

    PubMed  Google Scholar 

  • Goodman JL, Jurkovich P, Kramber JM, Johnson RC (1991) Molecular detection of persistant Borrelia burgdorferi in the urine of patients with active Lyme disease. Infect Immun 59:269–278

    PubMed  CAS  Google Scholar 

  • Grodzicki RL, Steere AC (1988) Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. J Infect Dis 157:790–797

    PubMed  CAS  Google Scholar 

  • Hansen K, Asbrink E (1989) Serodiagnosis of erythema migrans and acrodermatitis chronica atrophicans by the Borrelia burgdorferi flagellum enzyme-linked immunosorbent assay. J Clin Microbiol 27:545–551

    PubMed  CAS  Google Scholar 

  • Hansen K, Bangsborg JM, Fjordvang H, Pedersen NS, Hindersson P (1988a) Immunochemical characterization and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. Infect Immun 56:2047–2053

    PubMed  CAS  Google Scholar 

  • Hansen K, Hindersson P, Pedersen NS (1988b) Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease. J Clin Microbiol 26:338–346

    PubMed  CAS  Google Scholar 

  • Hansen K, Cruz M, Link H (1990) Oligoclonal Borrelia burgdorferi-specific IgG antibodies in cerebrospinal fluid in Lyme neuroborreliosis. J Infect Dis 161:1194–1202

    PubMed  CAS  Google Scholar 

  • Hansen K, Pii K, Lebech A-M (1991) Improved immunoglobulin M serodiagnosis in Lyme borreliosis by using a µ-capture enzyme-linked immunosorbent assay with biotinylated Borrelia burgdorferi flagella. J Clin Microbiol 291:166–173

    Google Scholar 

  • Hechemy KE, Harris HL, Duerr MJ, Benach JL, Reimer CB (1988) Immunoglobulin G subclasses specific to Borrelia burgdorferi in patients with Lyme disease. Ann NY Acad Sci 539:162–169

    PubMed  CAS  Google Scholar 

  • Herzer P, Wilske B (1986) Lyme arthritis in Germany. Zentralbl Bakteriol Hyg (A) 263:268–274

    CAS  Google Scholar 

  • Herzer P, Wilske B, Preac-Mursic V, Schierz G, Schattenkirchner M, Zöllner N (1986) Lyme arthritis: clinical features, serological and radiographic findings of cases in Germany. Klin Wochenschr 64:206–215

    PubMed  CAS  Google Scholar 

  • Herzer P, Wilske B, Schewe S, Preac-Mursic V, Schierz G (1989) Serologische Diagnostik der Lyme-Arthritis. Aktuel Rheumatol 14:125–129

    Google Scholar 

  • Hofstad H, Matre R, Nyland H, Ulvestad E (1987) Bannwarth’s syndrome: serum and CSF IgG antibodies against Borrelia burgdorferi examined by ELISA. Acta Neurol Scand 75:37–45

    PubMed  CAS  Google Scholar 

  • Hyde FW, Johnson RC, White TJ, Shelburne CE (1989) Detection of antigens in urine of mice and humans infected with Borrelia burgdorferi, etiologic agent of Lyme disease. J Clin Microbiol 27:58–61

    PubMed  CAS  Google Scholar 

  • Johnson SE, Klein GC, Sehmid GP, Bowen GS, Feeley JC, Schulze T (1984) Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete. J Clin Microbiol 19:81–82

    PubMed  CAS  Google Scholar 

  • Jonsson M, Bergström S (1990) Molecular characteristics and genetic organization of the osp operon from different European Borrelia burgdorferi strains. 4th international conference on Lyme borreliosis, Stockholm

    Google Scholar 

  • Karlsson M (1990a) Western immunoblot and flagellum enzyme-linked immunosorbent assay for serodiagnosis of Lyme borreliosis. J Clin Microbiol 28:2148–2150

    PubMed  CAS  Google Scholar 

  • Karlsson M, Hovind-Hougen K, Svenungsson B, Stiernstedt G (1990b) Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis. J Clin Microbiol 28:473–479

    PubMed  CAS  Google Scholar 

  • Kmety E, Rehacek J, Vyrostekova V (1986) Investigation of ticks for the presence of Borrelia in Czechoslovakia. Zentralbl Bakteriol Hyg (A) 263:468–470

    Google Scholar 

  • Kristoferitsch W, Sterck AJ, Murray N, Stanek G, Lanschützer H (1986) Oligoclonal antibodies in CSF of patients with meningopolyneuritis Garin-Bujadoux-Bannwarth: Ig class, light chain type and specifity. Zentralbl Bakteriol Hyg (A) 263:307–313

    Google Scholar 

  • LeFebvre RB, Perng G-C, Johnson RC (1990) The 83-kilodalton antigen of Borrelia burgdorferi which stimulates immunoglobulin M (IgM) and IgG responses in infected hosts is expressed by a chromosomal gene. J Clin Microbiol 28:1673–1676

    PubMed  CAS  Google Scholar 

  • Link H, Baig S, Olsson T, Zachau A, Stiernstedt G (1988) B-cell response at a cellular level in cerebrospinal fluid and blood in Lyme disease and controls. Ann NY Acad Sci 539:389–392

    Google Scholar 

  • Luft BJ, Jiang W, Munoz P, Dattwyler RJ, Gorevic PD (1989) Biochemical and immunological characterization of the surface proteins of Borrelia burgdorferi Infect Immun 57:3637–3645

    PubMed  CAS  Google Scholar 

  • Luther B, Moskophidis M (1990) Antigenic cross-reactivity between Borrelia burgdorferi, Borellia recurrentis, Treponema pallidum and Treponema phagedenis Zentralbl Bakteriol 274:214–226

    PubMed  CAS  Google Scholar 

  • MacDonald AB (1986) Human fetal borreliosis, toxemia of pregnancy, and fetal death. Zentralbl Bakteriol Hyg (A) 263:189–200

    CAS  Google Scholar 

  • Magnarelli LA (1988) Serological diagnosis of Lyme disease. Ann NY Acad Sci 539:154–161

    PubMed  CAS  Google Scholar 

  • Magnarelli LA, Anderson JF (1988) Enzyme-linked immunosorbent assays for detection of class-specific immunoglobulins to Borrelia burgdorferi Am J Epidemiol 127:818–825

    PubMed  CAS  Google Scholar 

  • Magnarelli LA, Meegan JM, Anderson JF, Chappell WA (1984) Comparison of an indirect fluorescent antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease. J Clin Microbiol 20:181–184

    PubMed  CAS  Google Scholar 

  • Magnarelli LA, Anderson JF, Johnson RC (1987) Cross-reactivity in serological tests for Lyme disease and other spirochetal infections. J Infect Dis 156:183–188

    PubMed  CAS  Google Scholar 

  • Martin R, Martens U, Krüger H, Sticht-Groh V, Dörries R (1987) Nachweis und Identifizierung Borrelia-burgdorferi-spezifischer, oligoklonarer Immunglobuline (IgG) im unkonzentrierten Liquor mit der Immunoblottechnik. In: Peock K, Hacke W, Schneider R, (eds) Neuroimmunologie, spinale Krankheiten, Neuropsychologic, metabolische Enzephalopathien, neurologische Notfälle, interventionelle Neuroradiologie. Springer, Berlin Heidelberg New York, pp 440–442 (Verhandlungen der Deutschen Gesellschaft für Neurologie, vol 4)

    Google Scholar 

  • Martin R, Martens U, Sticht-Groh V, Dörries R, Krüger H (1988) Persistent intrathecal secretion of oligoclonal, Borrelia burgdorferi-specific IgG in chronic meningoradiculomyelitis. J Neurol 225:229–239

    Google Scholar 

  • Müller F (1982) Der 19S-IgM-FTA-Abs-Test in der Serodiagnostik der Syphilis. Technik, Fehlermöglichkeiten und diagnostische Aussagen. Immun Infekt 10:32–34

    Google Scholar 

  • Müller F, Moskophidis M (1983) Estimation of the local production of antibodies to Treponema pallidum in the central nervous system of patients with neurosyphilis. Br J Vener Dis 59:80–84

    PubMed  Google Scholar 

  • Neubert U, Krampitz HE, Engl H (1986) Microbiological findings in erythema (chronicum) migrans and related disorders. Zentralbl Bakteriol Hyg (A) 263:237–252

    CAS  Google Scholar 

  • Park HK, Jones BE, Barbour AG (1986) Erythema chronicum migrans of Lyme disease: diagnosis by monoclonal antibodies. J Am Acad Dermatol 15:406–410

    PubMed  CAS  Google Scholar 

  • Pfister HW, Neubert U, Wilske B, Preac-Mursic V, Einhäupl KM, Borasio GD (1986) Reinfection with Borrelia burgdorferi Lancet II:984–985

    Google Scholar 

  • Preac-Mursic V, Schierz G, Pfister H-W, Einhäupl K, Wilske B, Weber K (1984a) Isolierung einer Spirochäte aus Liquor cerebrospinalis bei Meningoradiculitis-Bannwarth. Munch Med Wochenschr 126:275–276

    Google Scholar 

  • Preac-Mursic V, Wilske B, Schierz G, Pfister H-W, Einhäupl K (1984b) Repeated isolation of spirochetes from the cerebrospinal fluid of a patient with meningoradiculitis Bannwarth. Eur J Clin Microbiol 3:564–565

    PubMed  CAS  Google Scholar 

  • Preac-Mursic V, Wilske B, Herzer P, Schierz G, Bauer H (1985) Acrodermatitis chronica atrophicans: eine Borreliose! Hautarzt 36:691–693

    PubMed  CAS  Google Scholar 

  • Preac-Mursic V, Wilske B, Schierz G (1986) European Borrelia burgdorferi isolated from humans and ticks: Culture conditions and antibiotic susceptibility. Zentralbl Bakteriol Hyg (A) 263:112–118

    CAS  Google Scholar 

  • Preac-Mursic V, Weber K, Pfister HW, Wilske B, Gross B, Baumann A, Prokop J (1989) Survival ofBorrelia burgdorferi in antibiotically treated patients with Lyme borreliosis. Infection 17:355–359

    PubMed  CAS  Google Scholar 

  • Preac-Mursic V, Patsouris E, Wilske B, Reinhardt S, Groß B, Mehrain P (1990) Persistence of Borrelia burgdorferi and histopathological alterations in experimentally infected animals; comparison with histopathological findings in human Lyme disease. Infection 18:332–341

    PubMed  CAS  Google Scholar 

  • Rehse-Küpper B, Ackermann R (1986) Demonstration of locally synthesized borrelia antibodies in cerebrospinal fluid. Zentralbl Bakteriol Hyg (A) 263:407–411

    Google Scholar 

  • Reimers CD, Pongratz DE, Neubert U, Pilz A, Hübner G, Naegele M, Wilske B, Duray PH, de Koning JC (1989) Myositis caused by B. burgdorferi: report of four cases. J Neuorol Sci 91:215–226

    CAS  Google Scholar 

  • Rosa PA, Schwan TG (1989) A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. J Infect Dis 160:1018–1029

    PubMed  CAS  Google Scholar 

  • Röther E, Vogt A, Meyer-Düttingdorf E, Peter HH (1988) Wertigkeit der Serodiagnostik für Verlauf und Therapie der Borreliose. Immun Infekt 16:27–28

    PubMed  Google Scholar 

  • Russel H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B (1984) Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis 149:465–470

    Google Scholar 

  • Schmidli J, Hunziker T, Moesli P, Schaad UB (1988) Cultivation of Borrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis. J Infect Dis 158:905–906

    PubMed  CAS  Google Scholar 

  • Schwan TG, Burgdorfer W (1987) Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation. J Infect Dis 156:852–853

    PubMed  CAS  Google Scholar 

  • Schwan TG, Burgdorfer W, Garon CF (1988) Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of cultivation. Infect Immun 56:1831–1836

    PubMed  CAS  Google Scholar 

  • Schwan TG, Simpson WJ, Schrumpf ME, Karstens RH (1989) Identification of Borrelia burgdorferi andB. hermsii using DNA hybridization probes. J Clin Microbiol 27:1734–1738

    PubMed  CAS  Google Scholar 

  • Simpson WJ, Garon CF, Schwan TG (1990a) Borrelia burgdorferi contains DNA sequences that are species specific and plasmid associated. Infect Immun 58:847–853

    PubMed  CAS  Google Scholar 

  • Simpson WJ, Schrumpf ME, Schwan TG (1990b) Reactivity of human Lyme borreliosis sera with a 39-kilodalton antigen specific to Borrelia burgdorferi Clin Microbiol 28:1329–1337

    CAS  Google Scholar 

  • Simpson WJ, Burgdorfer W, Schrumpf ME, Karstens RH, Schwan TG (1991) Antibody to a 39-kilodalton Borrelia burgdorferi antigen (P39) as a marker for infection in experimentally and naturally inoculated animals. J Clin Microbiol 29:236–243

    PubMed  CAS  Google Scholar 

  • Stanek G, Wewalka G, Groh V, Neumann R (1985) Isolation of spirochetes from the skin of patients with erythema chronicum migrans in Austria. Zentralbl Bakteriol Hyg (A) 260:88–90

    CAS  Google Scholar 

  • Stanek G, Hirschl A, Kristoferitsch W (1986) EFA and ELISA in serodiagnosis of Lyme borreliosis. Mitt Österr Ges Trop Parasitol 8:1–6

    Google Scholar 

  • Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE (1983) The spirochetal etiology of Lyme disease. N Engl J Med 308:733–740

    PubMed  CAS  Google Scholar 

  • Stiernstedt G, Sköldenberg B, Vandvik B, Hederstedt B, GÃ¥rde A, Kolmodin G, Jörbäck H, Svenungsson B (1984) Chronic meningitis and Lyme disease in Sweden. Yale J Biol Med 57:491–497

    PubMed  CAS  Google Scholar 

  • Stiernstedt G, Granström M, Hederstedt B, Sköldenberg B (1985) Diagnosis of spirochetal meningitis by enzyme-linked immunosorbent assay and indirect immunofluorescent assay in serum and cerebrospinal fluid. J Clin Microbiol 21:819–825

    PubMed  CAS  Google Scholar 

  • Stiernstedt G, Eriksson G, Enfors W, Jörbeck H, Svenungson B, Sköldenberg B, Granström M (1986) Erythema chronicum migrans in Sweden: clinical manifestations and antibodies to Ixodes ricinus spirochete measured by indirect immunofluorescence and enzyme-linked immunosorbent assay. Scand J Infect Dis 18:217–224

    PubMed  CAS  Google Scholar 

  • Wallich R, Schaible UE, Simon MM, Heiberger A, Kramer MD (1989) Cloning and sequencing of the gene encoding the outer surface protein A (OspA) of a European Borrelia burgdorferi isolate. Nucleic Acids Res 17:8864

    PubMed  CAS  Google Scholar 

  • Wallich R, Moter SE, Simon MM, Ebnet K, Heiberger A, Kramer MD (1990) The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): Molecular cloning, expression, and amplification of the gene. Infect Immun 58:1711–1719

    PubMed  CAS  Google Scholar 

  • Wassmann K, Borg-von Zepelin M, Zimmermann O, Stadler M, Eiffert H, Thomssen R (1989) Determination of immunoglobulin M antibody against Borrelia burgdorferi to differentiate between acute and past infections. Zentralbl Bacteriol (Suppl) 18:281–289

    Google Scholar 

  • Weber K, Schierz G, Wilske B, Preac-Mursic V (1984a) Zur Klinik und Ätiologie der Acrodermatitis chronica atrophicans. Hautarzt 35:571–577

    PubMed  CAS  Google Scholar 

  • Weber K, Schierz G, Wilske B, Preac-Mursic V (1984b) European erythema migrans disease and related disorders. Yale J Biol Med 57:463–471

    PubMed  CAS  Google Scholar 

  • Weber K, Schierz G, Wilske B, Preac-Mursic V (1985) Das Lymphozytom - eine Borreliose? Z Hautkr 60:1585–1598

    PubMed  CAS  Google Scholar 

  • Weber K, Schierz G, Wilske B, Neubert U, Krampitz HE, Barbour AG, Burgdorfer W (1986) Reinfection in erythema migrans disease. Infection 14:32–35

    PubMed  CAS  Google Scholar 

  • Weber K, Preac-Mursic V, Neubert U, Thurmayr R, Herzer P, Wilske B, Schierz G, Marget W (1988a) Antibiotic therapy of early European Lyme borreliosis and acrodermatitis chronica atrophicans. Ann NY Acad Sci 539:324–345

    PubMed  CAS  Google Scholar 

  • Weber K, Preac-Mursic V, Reimers CD (1988b) Spirochetes isolated from two patients with morphea. Infection 16:25–26

    PubMed  CAS  Google Scholar 

  • Wilske B, Schierz G, Preac-Mursic V, Weber K, Pfister H-W, Einhäupl K (1984) Serological diagnosis of erythema migrans disease and related disorders. Infection 5:331–337

    Google Scholar 

  • Wilske B, Münchhoff P, Schierz G, Preac-Mursic V, Roggendorf M, Zoulek G (1985) Zur Epidemiologie der Borrelia-burgdorferi-lnfektion bei Waldarbeitern aus Oberbayern. Munch Med Wochenschr 127:171–172

    Google Scholar 

  • Wilske B, Preac-Mursic V, Schierz G, von Busch K (1986a) Immunochemical and immunological analysis of European Borrelia burgdorferi strains. Zentralbl Bakteriol Hyg (A) 263:92–102

    CAS  Google Scholar 

  • Wilske B, Schierz G, Preac-Mursic V, von Busch K, Kühbeck R, Pfister HW, Einhäupl K (1986b) Intrathecal production of specific antibodies against Borrelia burgdorferi in patients with lymphocytic meningoradiculitis (Bannwarth’s syndrome). J Infect Dis 153:304–314

    PubMed  CAS  Google Scholar 

  • Wilske B, Schierz G, Preac-Mursic V, Bader L, Pfister HW, Einhäupl K, Herzer P (1987) Intrathekale Produktion spezifischer Antikörper gegen Borrelia burgdorferi bei Patienten mit Bannwarth-Syndrome. In: Poeck K, Hacke W, Schneider R (eds) Neuroimmunologie, spinale Krankheiten, Neuropsychologic, metabolische Enzephalopathien, neurologische Notfälle, interventionelle Neuroradiologie. Springer, Berlin Heidelberg New York, pp 443–447 (Verhandlungen der Deutschen Gesellschaft für Neurologie, vol 4)

    Google Scholar 

  • Wilske B, Preac-Mursic V, Schierz G, Gueye W, Herzer P, Weber K (1988a) Immunochemische Analyse der Immunantwort bei Spätmanifestationen der Lyme Borreliose. (Immunochemical analysis of the immune response in late manifestations of Lyme borreliosis) Zentralbl Bakteriol Hyg (A) 267:549–558

    CAS  Google Scholar 

  • Wilske B, Preac-Mursic V, Schierz G, Kühbeck R, Barbour AG, Kramer M (1988b) Antigenic variability of Borrelia burgdorferi Ann NY Acad Sci 539:126–143

    PubMed  CAS  Google Scholar 

  • Wilske B, Fuchs R, Schierz G, Preac-Mursic V, Liegl G (1989a) Immunodominant proteins for the humoral immune response in Lyme borreliosis (poster). In: European Society of Clinical Microbiology and Infectious Diseases (ed) 4th European congress of clinical microbiology. Nizza, pp 911/PP43

    Google Scholar 

  • Wilske B, Preac-Mursic V, Schierz G, Liegl G, Gueye W (1989b) Detection of IgM- and IgG antibodies to Borrelia burgdorferi using different strains as antigen. Zentralbl Bakteriol (Suppl) 18:299–309

    Google Scholar 

  • Wilske B, Schierz G, Preac-Mursic V, Pfister HW, Weber K, von Busch K, Baruschke A (1989c) IgM- and IgG immune response to Borrelia burgdorferi in erythema migrans and neuroborreliosis. Zentralbl Bakteriol (Suppl) 18:290–298

    Google Scholar 

  • Wilske B, Preac-Mursic V, Fuchs R, Bruckbauer H, Hofmann A, Zumstein G, Jauris S, Soutschek E, Motz M (1990) Immundominant proteins of Borrelia burgdorferi; implications for improving serodiagnosis of Lyme borreliosis. In: Neu HC (ed) New antibacterial strategies. Churchill Livingstone, London

    Google Scholar 

  • Wilske B, Anderson JF, Baranton G, Barbour AG, Hoving-Hougen K, Johnson RC, Preac-Mursic V (1991) Workshop summary: taxonomy of Borrelia spp. Scand J Infect Dis Suppl 77:108–129

    PubMed  CAS  Google Scholar 

  • Wilske B, Barbour AG, Bergström S, Burman N, Restrepo BI, Rosa PA, Schwan T, Soutschek E, Wallich R (1992) Antigenic variation and strain heterogeneity in Borrelia spp. Res Microbiol 143:in press

    Google Scholar 

  • Ziegelmeier R, Bieker R, Behrens F, Vermeer H (1983) Diagnostik akuter Infektionen: IgM-Antikörperbestimmung ohne Rheumafaktor-Interferenz. Laboratoriumsblätter 33:19–25

    Google Scholar 

  • Zöller L, Burkard S, Schäfer H (1991) Validity of Western immunoblot band patterns in the serodiagnosis of Lyme borreliosis. J Clin Microbiol 29:174–182

    PubMed  Google Scholar 

  • Zumstein G, Fuchs R, Preac-Mursic V, Wilske B, Soutschek E (1990) Identification and characterisation by restriction endonucleases of the outer surface protein OspA of Borrelia burgdorferi (Poster). In: 4th international conference on Lyme Borreliosis, Stockholm

    Google Scholar 

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Wilske, B., Preac-Mursic, V. (1993). Microbiological Diagnosis of Lyme Borreliosis. In: Weber, K., Burgdorfer, W., Schierz, G. (eds) Aspects of Lyme Borreliosis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77614-4_21

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