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Three-Way Differential Staining of Chromosomes for the Identification of SCEs per Cell Cycle: Fundamentals and Applications

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Advances in Mutagenesis Research

Part of the book series: Advances in Mutagenesis Research ((MUTAGENESIS,volume 4))

Abstract

Sister chromatid exchanges (SCEs) are usually scored in cells which have incorporated 5-bromodeoxyuridine (BrdUrd) for two cell cycles or, alternatively, for just one cell cycle followed by another in the absence of the halogenated nucleoside. By using this approach, however, the yield of SCEs observed at second mitosis is the sum of exchanges which occur in either of the two cell cycles. Therefore, it is impossible to distinguish which SCEs took place during the first or the second cell cycle.

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  1. Department of Cell Biology, Faculty of Biology, E-41012, Seville, Spain

    F. Cortés & P. Escalza

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  1. F. Cortés
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  1. FB9 der Universität Gesamthochschule Essen, Universitätsstraße 5, Postfach 10 37 64, 4300, Essen 1, Germany

    Günter Obe

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Cortés, F., Escalza, P. (1993). Three-Way Differential Staining of Chromosomes for the Identification of SCEs per Cell Cycle: Fundamentals and Applications. In: Obe, G. (eds) Advances in Mutagenesis Research. Advances in Mutagenesis Research, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77466-9_8

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