Abstract
Three major systems of genetic transfer are recognized in bacteria: conjugation, transduction, and transformation. These systems have been used routinely in the laboratory as tools of genetic analysis for many years, but their importance in investigating microbial genetic diversity and evolution in natural habitats has only recently begun to be investigated (Levy and Miller 1989). To date, most environmental studies have focussed on the potential for conjugation to transfer extra-chromosomal elements among microbes of the same or different species (Sayre and Miller 1991). Transduction (virus-mediated, horizontal gene transfer) has often been discounted as a potentially important process for the redistribution of genetic information (both chromosomal and extra-chromosomal) in bacterial populations because it is reductive (i.e., the donor is killed in the process of donating its genetic material to the recipient). However, recent reports have documented that transduction can be a fertile gene exchange system in natural ecosystems (Kokjohn 1989, Kokjohn and Miller 1992). We are using Pseudomonas aeruginosa as a model organism to study virus-mediated gene transfer in freshwater microbial populations (Morrison et al. 1978; Saye et al. 1987; 1990; Saye and Miller 1989; Miller et al. 1990; Ripp et al. 1992). Our studies have revealed a significant potential for transduction of both plasmid and chromosomal DNA in these environments.
Keywords
- Hydraulic Residence Time
- Natural Microbial Community
- Genetically Engineer Microorganism
- Natural Microbial Population
- Recipient Bacterium
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Amin MK, Day MJ (1988) Donor and recipient effects on transduction frequency in situ. REGEM 1 Program, p. 2.
Baross JH, Liston J, Morita RY (1974) Some implication of genetic exchange among marine vibrios, including Vibrio parahaemolyticus, naturally occurring in the Pacific oyster. In Fujio T, Sakaguchi G, Sakazaki R, Takeda Y (eds.) International Symposium on Vibrio parahaemolyticus. Saikon Pub. Co. Ltd., Tokyo, pp. 129–137.
Jarolmen H, Bonke A, Crowell RL (1965) Transduction of Staphylococcus aureus to tetracyclin resistance in vivo. J Bacteriol 89:1286–1290.
Kidambi SP, Ripp S, Miller RV (1992) Evidence for phage-mediated gene transfer among Pseudomonas aeruginosa on the phylloplane. Submitted.
Kokjohn TA (1989) Transduction: mechanism and potential for gene transfer in the environment. In Levy SB, Miller RV (eds.), Gene Transfer in the Environment. McGraw-Hill, New York, pp. 73–98.
Kokjohn TA, Miller RV (1992) Gene transfer in the environment: transduction. In Fry JC, Day MJ (eds.), Release of Genetically Engineered and Other Microorganisms, Edward Arnold, London, in press.
Kokjohn TA, Sayler GS, Miller RV (1991) Attachment and replication of Pseudomonas aeruginosa bacteriophages under conditions simulating aquatic environments. J Gen Microbiol 137:661–666.
Levy SB, Miller RV (1989) Gene Transfer in the Environment. McGraw-Hill, New York.
Miller RV, Kokjohn TA, Sayler GS (1990). Environmental and molecular characterization of systems which affect genome alteration in Pseudomonas. In Silver S, Chakrabarty AM, Iglewski B, Kaplan S (eds.), Pseudomonas: Biotransformations, Pathogenesis, and Evolving Biotechnology. American Society for Microbiology, Washington, D. C., pp. 252–268.
Miller RV, Pemberton JM, Richards KE (1974) F116, D3, and G101: temperate bacteriophages of Pseudomonas aeruginosa. Virology 59:566–569.
Miller RV, Pemberton JM, Clark AJ (1976) Prophage F116: evidence for extrachromosomal location in Pseudomonas aeruginosa strain PAO. J Virol 22:844–847.
Miller RV, Sayler GS (1992) Bacteriophage-host interactions in aquatic systems. In Wellington EM, van Elsas JD (eds.), Genetic Interactions Among Microorganisms in the Natural Environment, Pergamon Press, Oxford, UK, pp. 176–193.
Morrison WD, Miller RV, Sayler GS (1978) Frequency of F116 mediated transduction of Pseudomonas aeruginosa in a freshwater environment. Appl Environ Microbiol 36:724–730.
Novick RP, Edelman I, Lofdahl S (1986) SmallStaphylococcus aureus plasmids are transduced as linear multimers that are formed and resolved by replicative processes. J Mol Biol 192:209–220.
Novick RP, Morse SI (1967)In vivo transmission of drug resistance factors between strains ofStaphylococcus aureus. J Expt Med 125:45–59.
Ogunseitan OA, Sayler GS, Miller RV (1990) Dynamic interaction of Pseudomonas aeruginosa and bacteriophages in lake water. Microb Ecol 19:171–185.
Ogunseitan OA, Sayler GS, Miller RV (1992) Application of DNA probes to the analysis of bacteriophage distribution patterns in the environment. Appl Environ Microbiol, in press.
Osman MA, Gealt MA (1988) Wastewater bacteriophages transduce genes from the chromosome and a recombinant plasmid Abst. Annl Meet Am Soc Microbiol p. 254.
Replicon J, Miller RV (1990) Modeling the potential for transduction to stabilize a foreign genotype within an established microbial community. Abst VHIth Internat Cong Virol, p. 117.
Ripp S, Ogunseitan OA, Miller RV (1992) Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1. Submitted
Romig WR, Brodetsky AM (1961) Isolation and preliminary characterization of bacteriophages of Bacillus subtilis. J Bacteriol 82:135–141.
Saye DJ, Miller RV (1989) Gene transfer in aquatic environments. In Levy SB, Miller RV (eds.), Gene Transfer in the Environment, McGraw-Hill, New York, p. 223–254.
Saye DJ, Ogunseitan OA, Sayler GS, Miller RV (1990) Transduction of linked chromosomal genes between Pseudomonas aeruginosa during incubation in situ in a freshwater habitat. Appl Environ Microbiol 56:140–145.
Saye DJ, Ogunseitan O, Sayler GS, Miller RV (1987) Potential for transduction of plasmids in a natural freshwater environment: effect of plasmid donor concentration and a natural microbial community on transduction in Pseudomonas aeruginosa. Appl Environ Microbiol 53:987–995.
Saye DJ, O’Morchoe SB (1992) Evaluating the potential for genetic exchange in natural freshwater environments. In Levin M, Seidler R, Rogul M (eds.), Microbial Ecology: Principles, Methods, and Application in Environmental Biotechnology. McGraw-Hill, NewYork, pp. 283–309.
Sayre PG, Miller RV (1991) Bacterial mobile genetic elements: importance in assessing the environmental fate of genetically engineered sequences. Plasmid 26:151–171.
Stotzky G (1989) Gene transfer among bacteria in soil. In Levy SB, Miller RV (eds.), Gene Transfer in the Environment, McGraw- Hill, NewYork, pp. 165–222.
Zeph LR, Stotzky G (1989) Use of a biotinylated DNA probe to detect bacteria transduced by bacteriophage PI in soil. Appl Environ Microbiol 5:661–665.
Zeph LR, Onaga MA, Stotzky G (1988) Transduction of Escherichia coli by bacteriophage PI in soil. Appl Environ Microbiol 54:1731–1737.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Miller, R.V., Ripp, S., Replicon, J., Ogunseitan, O.A., Kokjohn, T.A. (1992). Virus-Mediated Gene Transfer in Freshwater Environments. In: Gauthier, M.J. (eds) Gene Transfers and Environment. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77450-8_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-77450-8_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77452-2
Online ISBN: 978-3-642-77450-8
eBook Packages: Springer Book Archive