Stress and Survival in Alcaligenes eutrophus CH34: Effects of Temperature and Genetic Rearrangements
Alcaligenes eutrophus, a chemolithotrophic Gram-negative bacterium, is a good colonizer of polluted soils perfectly apt to survive in a variety of harsh conditions (Diels et al. 1989; Diels and Mergeay 1990). Therefore, strains of this species attracted our interest for various environmental applications. Using recombinant A.eutrophus CH34 (ATCC 43123) and A. eutrophus A5 strains, we were able to obtain concomitant expression of xenobiotic degrading genes (derived from the A5 chromosome) and heavy metal resistances encoded by the CH34 megaplasmids pMOL28 and pMOL30 (Mergeay et al. 1985; Springael Ph.D. thesis). Such strains look promising in bioremediation processes. In addition, research is going on to use CH34 or related strains in water and soil depollution and metal reclamation. Alcaligenes eutrophus was not only shown to be a good host for the expression of foreign genes, but also easily accessible for genetic manipulation by means of conjugaison. Having in mind to use A. eutrophus as a containment system for the release of recombinant DNA in the environment, this bacteria was also used as a recipient in a study to establish a model system assaying the intergeneric transmission and expression of cloned genes in soil samples (Top et al. 1990).
KeywordsIntergeneric Transmission Mutation Mechanism Heavy Metal Resistance Strain CH34 Containment System
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- Top E, Mergeay M, Springael D, Verstraete W (1990) Gene escape model: transfer of heavy metal resistance genes from Escherichia coli to Alcaligenes eutrophus on agar plates and in soil samples. Appl Environ Microbiol 56(8):2471–2479.Google Scholar