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The Detection of Marked Populations of Recombinant Pseudomonads Released into Lake Water

  • C. Winstanley
  • J. A. W. Morgan
  • J. R. Saunders
  • R. W. Pickup

Abstract

Some of the more esoteric catabolic activities exhibited by bacteria in their battle to degrade recalcitrant compounds, many of which are man-made pollutants, have been observed and studied extensively in species of Pseudomonas. Much research has been concentrated on elucidating the mechanisms of the catabolic pathways involved, with the result that many of the genes, often plasmid-borne, have been cloned and their regulatory systems resolved. The advance of recombinant DNA technology has allowed the manipulation of such catabolic activities to improve efficiency or range. This has encouraged the suggestion that genetically-modified microorganisms may be employed in the environment to degrade pollutants in situ. The success of such operations depends largely on the ability of laboratory-manipulated strains to survive and function following release into an unwelcoming environment.

Keywords

Lake Water Pseudomonas Putida Filter Water Sample Marked Population Marker Cassette 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Morgan JAW, Winstanley C, Pickup RW, Jones JG, Saunders JR (1989) Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water. Appl Environ Microbiol 55:2537–2544PubMedGoogle Scholar
  2. Morgan JAW, Winstanley C, Pickup RW, Saunders JR (1991) Rapid immunocapture of Pseudomonas putida cells from lake water by using bacterial flagella. Appl Environ Microbiol 57:503–509PubMedGoogle Scholar
  3. Winstanley C, Morgan JAW, Pickup RW, Jones JG, Saunders JR (1989) Differential regulation of lambda pL and pR promoters by a cI repressor in a broad-host-range thermoregulated plasmid marker system. Appl Environ Microbiol 55:771–777PubMedGoogle Scholar
  4. Winstanley C, Morgan JAW, Pickup RW, Saunders JR (1991) Use of a xylE marker gene to monitor survival of recombinant Pseudomonas putida populations in lake water by culture on nonselective media. Appl Environ Microbiol 57:1905–1913PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • C. Winstanley
    • 1
  • J. A. W. Morgan
    • 1
    • 2
  • J. R. Saunders
    • 1
  • R. W. Pickup
    • 1
    • 2
  1. 1.Department of Genetics and MicrobiologyUniversity of LiverpoolLiverpoolUK
  2. 2.Institute of Freshwater EcologyAmbleside, CumbriaUK

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