Conjugal Transfer of Genetic Information in Gnotobiotic Mice

  • F. Doucet-Populaire
Conference paper


Dissemination of antibiotic resistance determinants has greatly increased over the past two decades, and its spread is undoubtedly a response to the increasing use of antimicrobial agents. Antibiotic resistance genes therefore constitute a suitable system to study the extent of horizontal gene transfer among prokaryotes. In a attempt to elucidate the mechanism of in vivo dissemination of genetic information between phylogenetically remote organisms, we have studied the transfer of two different genetic elements in an animal model. Since the intestinal ecosystem is the most probable meeting point for the bacterial species studied in nature, donors and recipients were associated for 35 or 40 days in the digestive tract of gnotobiotic mice. The transfer was conducted in groups of 6 adult germ-free C3H mice (Centre de selection des animaux de laboratoire, Orléans, France). The mice were maintained in Trexler- type isolators. Animals were fed at libitum with a commercial diet sterilized by gamma irradiation. They were supplied with autoclaved drinking water acidified to pH 3 to prevent any bacterial growth in the beverage. After 12 hours without water, germ-free mice were inoculated intragastrically with 1 ml of a broth culture containing 108 colony forming units (CFU) of the recipient strain.


Listeria Monocytogenes Enterococcus Faecalis Conjugal Transfer Kanamycin Resistance Gene Transfer Frequency 
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  1. Andremont A, Gerbaud G, Tancrede C, Courvalin P (1985) Plasmid-mediated susceptibility to intestinal microbial antagonisms in Escherichia coli. Infect Immun 49:751–755PubMedGoogle Scholar
  2. Courvalin P, Carlier C (1986) Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet 205:291–297PubMedCrossRefGoogle Scholar
  3. Doucet-Populaire F, Trieu-Cuot P, Dosbaa I, Andremont A, Courvalin P (1991) Inducible transfer of conjugative transposon Tn1545 from Enterococcus faecalis to Listeria monocytogenes in the digestive tracts of gnotobiotic mice. Antimicrob Agents Chemother 35:185–187PubMedGoogle Scholar
  4. Doucet-Populaire F, Trieu-Cuot P, Andremont A, Courvalin P (1992) Conjugal transfer of plasmid DNA fromEnterococcus faecalis to Escherichia coli in the digestive tracts of gnotobiotic mice. Antimicrob Agents Chemother 36:502–504.PubMedGoogle Scholar
  5. Freter R, Brickner H, Fekete J, Vickerman MM, Carcey KE (1983) Survival and implantation of Escherichia coli in the intestinal tract. Infect Immun 39:686–703.PubMedGoogle Scholar
  6. Trieu-Cuot P, Carlier C, Courvalin P (1988) Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli. J Bacteriol 170:4388–4391.PubMedGoogle Scholar
  7. Trieu-Cuot P, Arthur M, Courvalin P (1987) Transfer of genetic information between Gram-positive and Gram-negative under natural conditions, p.65–68. In R Curtiss III and JJ Feretti (ed.), Streptococcal genetics. American Society for Microbiology, Washington D CGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • F. Doucet-Populaire
    • 1
  1. 1.Laboratoire de BactériologieFaculté de Médecine Pitié-SalpêtrièreParis Cedex 13France

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