Abstract
Gene transfer in the environment has been identified as a process which potentially enhances putative hazards of released genetically engineered microorganisms (GEMs). This contentment has spurred recent research in this area. Our understanding of how the environment affects genetic interactions between micro-organisms has therefore increased drastically in recent years. It is now evident that the gene transfer mechanisms classically known to take place in vitro in the laboratory, i.e. transformation, transduction and conjugation, can also occur in nature given conditions are conducive to these processes (e.g. Stewart and Sinigalliano 1990; Zeph et al. 1988; Van Elsas et al. 1988).
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References
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. John Wiley & Sons, New York.
Bertram J, Strätz M, Dürre P (1991) Natural transfer of conjugative transposon Tn916 between Gram-positive and Gram-negative bacteria. J Bacteriol 173:443–448.
Chater KF, Henderson DJ, Bibb MJ, Hopwood DA (1988) Genome flux in Streptomyces coelicolor and other streptomycetes and its possible relevance to the evolution of mobile antibiotic resistance determinants, pp. 7–42. In Transposition (Kingsman AJ, Chater KF, Kingsman SM, Eds.) Cambridge University Press, Cambridge.
Fulthorpe RR, Wyndham RC (1991) transfer and expression of the catabolic plasmid pBR60 in wild bacterial recipients in a freshwater ecosystem. Appl Environ Microbiol 57:1546–1553.
Fry JC, Day MJ (1990) Plasmid transfer in the epilithon. pp. 55–80. In Bacterial Genetics in Natural Environments. (Fry JC, Day MJ, Eds) Chapman and Hall, London.
Gormley EP, Davies J (1991) Transfer of plasmid RSF1010 by conjugation fromEscherichia coli toStreptomyces lividans and Micobacterium smegmatis. J Bacteriol 173:6705–6708.
Henschke RB, Schmidt FRJ (1990) Plasmid mobilization from genetically engineered bacteria to members of the indigenous soil microflora in situ. Curr Microbiol 20:105–110.
Herrero M, De Lorenzo V, Timmis KN (1990) Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. Appl Environ Microbiol 172:6557–6567.
Hill KE, Weightman AJ, Fry JC (1992) Isolation and screening of plasmids from the epilithon which mobilize recombinant plasmid pDlO. Appl Environ Microbiol 58:1292–1300.
Klingmüller W (1991) Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter. FEMS Microbiol Ecol 85:107–116.
Krasovsky VN, Stotzky G (1987) Conjugation and genetic recombination in Escherichia coli in sterile and non-sterile soil. Soil Biol Biochem 19:631–638.
Manceau C, Gardan L, Devaux M (1986) Dynamics of RP4 transfer betweenXanthomonas campestris pv. Carolina and Erwinia herbicola in hazelnut tissues, in planta. Can J Microbiol 32:835–841.
Maniatis T, Fritsch EF, Sambrook AJ (1989) Molecular cloning: a laboratory manual, 2n edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
McClure, NC, Fry JC, Weightman AJ (1990) Gene transfer in activated sludge, pp. 111–129. In Bacterial genetics in natural environments (Fry JC, Day MJ, Ed.) Chapman and Hall, London.
Mergeay M, Lejeune P, Sadouk A, Gerits J, Fabry L (1987) Shuttle transfer (or retrotransfer) of chromosomal markers mediated by pULBl 13. Mol Gen Genet 209: 61–70.
Pretorius-Güth IM, Pühler A, Simon R (1990) Conjugal transfer of megaplasmid 2 betweenRhizobium meliloti strains in alfalfa nodules. Appl Environ Microbiol 56: 2354–2359.
Priefer UB, Simon R, Pühler A (1985) Extension of the host range of Escherichia coli vectors by incorperation of RSF1010 replication and mobilisation functions. J Bacteriol 163:324–330.
Richaume A, Angle JC, Sadowsky MJ (1990) Influence of soil variables on in situ plasmid transfer from Escherichia coli to Rhizobium fredii. Appl Environ Microbiol 55:1730–1734.
Schmidt FRJ, Nücken EJ, Henschke RB (1988) Nucleotide sequence analysis of 2“-aminoglycoside nucleotidyl-transferase ANT(2“) from TN4000: its relationship with aad(3“) and impact on Tn21 evolution. Mol Microbiol 2:709–717.
Simon R, Priefer U, Pühler A (1983) Vector plasmids for in-vivo and in-vitro manipulations of Gram-negative bacteria, pp. 89–106. In Molecular genetics of the bacteria-plant interaction (Pühler A, ed) Springer-Verlag, Berlin.
Smalla K, Cresswell N, Mendonca-Hagler LC, Wolters A, Van Elsas JD (1992) Rapid DNA extraction protocol from soil for polymerase chain reaction mediated amplification. J Appi Bacteriol (in press).
Smit E, Van Elsas JD, Van Veen JA, De Vos WM (1991) Detection of plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil by using bacteriophage ΦR2f for donor counterselection. Appl Environ Microbiol 57:3482–2488.
Stewart GJ, Sinigalliano CD (1990) Detection of horizontal gene transfer by natural transformation in native and introduced species of bacteria in marine and synthetic sediments. Appi Environ Microbiol 56:1818–1824.
Top E, Mergeay M, Springael D, Verstraete W (1990) Gene escape model: transfer of heavy metal resistance genes from Escherichia coli toAlcaligenes eutrophus on agar plates and in soil samples. Appl Environ Microbiol 56: 2471–2479.
Van Elsas JD, Trevors JT, Starodub ME (1988) Bacterial conjugation between pseudomonads in the rhizosphere of wheat. FEMS Microbiol Ecol 54:299–306.
Van Elsas JD, Govaert JM, Van Veen JA (1987) Transfer of plasmid pFT30 between bacilli in soil as influenced by bacterial population dynamics and soil conditions. Soil Biol Biochem 19:639–647.
Van Elsas JD, Van Overbeek LS, Fouchier R (1991) A specific marker, pat, for studying the fate of introduced bacteria and their DNA in soil using a combination of detection techniques. Plant and Soil 138:49–60.
Waalwijk C, Dullemans A, Maat C (1991) Construction of a bioinsecticidal rhizosphere isolate of Pseudomonas fluorescens. FEMS Microbiol Lett 77: 257–264.
Zeph LR, Onaga MA, Stozky G (1988) Transduction ofEscherichia coli by bacteriophage PI in soil. Appl Environ Microbiol 54, 190–193.
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© 1992 Springer-Verlag Berlin Heidelberg
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Smit, E., Van Elsas, J.D. (1992). Conjugal Gene Transfer in the Soil Environment; New Approaches and Developments. In: Gauthier, M.J. (eds) Gene Transfers and Environment. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77450-8_10
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DOI: https://doi.org/10.1007/978-3-642-77450-8_10
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