The influence of HLA-mismatches on phenotypic and functional characteristics of graft infiltrating lymphocytes after heart transplantation

  • A. J. Ouwehand
  • C. C. Baan
  • L. M. B. Vaessen
  • N. H. P. M. Jutte
  • A. H. M. M. Balk
  • E. Bos
  • F. H. J. Claas
  • W. Weimar
Conference paper

Abstract

We studied the influence of HLA mismatches on T lymphocyte cultures that were derived from endomyocardial biopsies (EMB) from 118 heart transplant recipients. From patients with DR mismatches, the majority of the EMB-derived cultures were dominated by CD4, while in patients without DR mismatches, CD8 was the predominant T cell subset. The majority (75%) of the cultures were cytotoxic against donor antigens. A significantly (P < 0.005) lower proportion of the cultures showed cytotoxicity (36%) against HLA-A antigens when compared to HLA-B (53%) or HLA-DR (49%). A dose effect phenomenon was detected for all HLA antigens, including HLA-A: a higher number of A, B or DR mismatches resulted in a higher number of cytotoxic cultures directed against these antigens. B and DR matching had the greatest influence on 6 month freedom from rejection. Both our experimental and clinical data indicated that HLA matching played a role in the immune response against a transplanted heart.

Key words

HLA matching Graft infiltrating cells Heart transplantation Alloreactivity Predominant phenotype 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • A. J. Ouwehand
    • 1
  • C. C. Baan
    • 2
  • L. M. B. Vaessen
    • 2
  • N. H. P. M. Jutte
    • 2
  • A. H. M. M. Balk
    • 3
  • E. Bos
    • 1
  • F. H. J. Claas
    • 4
  • W. Weimar
    • 3
  1. 1.Departments of Thoracic SurgeryErasmus University Rotterdam, University Hospital Rotterdam-DijkzigtRotterdamThe Netherlands
  2. 2.Departments of Internal Medicine IErasmus University Rotterdam, University Hospital Rotterdam-DijkzigtRotterdamThe Netherlands
  3. 3.Departments of CardiologyErasmus University Rotterdam, University Hospital Rotterdam-DijkzigtRotterdamThe Netherlands
  4. 4.Department of Immunohaematology and Blood BankUniversity Hospital LeidenThe Netherlands

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