Advertisement

Suppression of human lymphocyte proliferation and cytotoxic T lymphocyte generation by a soluble factor derived from K562 cells

  • K. Hayamizu
  • H. Yahata
  • K. Dohi
  • Y. Fukuda
Conference paper

Abstract

The human myeloid leukemia cell line, K562, secrets a lymphocyte growth-suppressive factor (LGSF). We report our investigation of the immunological and chemical features of this factor. LGSF showed no cytolytic effect on human peripheral blood mononuclear cells (PBMC). Nevertheless, LGSF adequately suppressed in vitro alloantigen- or mitogen (PHA, ConA, PWM)-stimu-lated human PBMC proliferation and alloantigen-in-duced cytotoxic T lymphocyte generation, in antigen-nonspecific and LGSF concentration-related manners. LGSF retained activity after filtration through a 0.22 |im filter membrane and storage at — 80 °C for 6 months. Ultrafiltration experiments indicated that suppressive activity was retained in the higher molecular weight fraction (MW > 100000) and that the activity was heat-labile at 56 °C for 30 min. These results strongly suggested that LGSF is a noncytotoxically immunosuppressive substance with a high molecular weight.

Key words

Immunosuppression Cytokine Human leukemia cell line Lymphocyte growth suppressive factor Cytotoxic Tlymphocyte 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dokhelar M, Testa U, Vainchenker W, Finale Y, Tetaud C, Salem P, Tursz T (1982) NK cell sensitivity of the leukemic K562 cells;Effect of sodium butyrate and hemin induction. J Immunol 128: 211–216PubMedGoogle Scholar
  2. 2.
    Kiczka W, Szkaradkiewicz A (1986) Suppression of monocyte mediated anti K562 cytotoxicity by soluble factors in the serum of cancer patients and in the supernatant of K562 cell culture. Immunol Lett 13:127–131PubMedCrossRefGoogle Scholar
  3. 3.
    Lozzio BB, Lozzio CB (1979) Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk Res 3:363–370PubMedCrossRefGoogle Scholar
  4. 4.
    Lozzio CB, Lozzio BB (1975) Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45:321–334PubMedGoogle Scholar
  5. 5.
    Olofsson T, Cline MJ (1978) Inhibitor of hematopoietic cell proliferation derived from a human leukemic cell line. Blood 52: 143–152PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • K. Hayamizu
    • 1
  • H. Yahata
    • 1
  • K. Dohi
    • 1
  • Y. Fukuda
    • 1
  1. 1.The Second Department of SurgeryHiroshima University School of MedicineHiroshimaJapan

Personalised recommendations