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Abdominal organ cluster transplantation in pigs and FK506

  • N. Kobayashi
  • K. Sakagami
  • S. Takasu
  • T. Matsuno
  • K. Orita
Conference paper

Abstract

Using a swine abdominal organ cluster transplantation model, we investigated the postoperative function and immunological reactions of a cluster graft and evaluated the immunosuppressive activity of FK506. The animals were divided into two groups. Group I (n = 6) served as controls, while in group II (n = 6) a daily dose of 0.1 mg/kg FK506 was given intramuscularly. Postoperative pancreatitis was the most important factor influencing the early outcome in both groups. In group I, the cause of late death was cachexia due to diabetes mellitus induced by pancreatic rejection. In group II, emaciation despite a well-functioning graft was the principal cause of late death. Histologically, in group I the grade of rejection in the pancreas was more severe than in the liver, and no sign of rejection was observed in group II. In conclusion, the pancreas suffered more severe rejection than the liver, and FK506 could significantly prevent cluster allograft rejection in this model.

Key words

Abdominal organ cluster transplantation Postoperative pancreatitis FK506 Diabetes mellitus pancreatic rejection 

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References

  1. 1.
    Calne RY, Sells RA, Pena JR, Davis DR, Millard PR, Herbertson BM, Binns RM, Davis DAL (1969) Induction of immunological tolerance by porcine liver allografts. Nature 223:472–476PubMedCrossRefGoogle Scholar
  2. 2.
    Kino T, Hatanaka H, Miyata S, Inamura N, Nishiyama M, Yajima T, Goto T, Okuhara M, Kohsaka M, Aoki H, Ochiai T (1987) A novel immunosuppressant isolated from a Streptomyces. II. Immunosuppressive effect of FK-506 in vitro. J Antibiot (Tokyo) 40: 1256–1265CrossRefGoogle Scholar
  3. 3.
    Kobayashi N, Sakagami K, Takasu S, Inagaki M, Hasuoka H, Yagi T, Onoda T, Matsuno T, Saito S, Kawamura T, Orita K (1991) Significance of pancreatic allograft rejection in swine abdominal organ cluster transplantation. Transplant Proc 23:635–639PubMedGoogle Scholar
  4. 4.
    Mieles L, Todo S, Tzakis A, Starzl TE (1990) Treatment of upper abdominal malignancies with organ cluster procedures. Clin Transplant 4:63–67PubMedGoogle Scholar
  5. 5.
    Ochiai T, Nagata M, Nakajima K, Suzuki T, Sakamoto K, Enomoto K, Gunji Y, Uematsu T, Goto T, Hori S, Kenmochi T, Nakagouri T, Asano T, Isono K, Hamaguchi K, Tsuchida H, Nakahara K, Inamura N, Goto T (1987) Studies of the effects of FK506 on renal allografting in the beagle dog. Transplantation 44:729–733PubMedCrossRefGoogle Scholar
  6. 6.
    Sakagami K, Kobayashi N, Nakagawa H, Saito S, Oiwa T, Orita K (1991) Experimental studies on a new splitting technique for simultaneous transplantation of liver/pancreas, duodenum and spleen in pigs: a preliminary report (in Japanese). Surg Therapy (in press)Google Scholar
  7. 7.
    Starzl TE, Todo S, Tzakis A, Podesta L, Mieles L, Demetris A, Teperman L, Selby R, Stevenson W, Stieber A, Gordon R, Iwatsuki S (1989) Abdominal organ cluster transplantation for the treatment of upper abdominal malignancies. Ann Surg 210: 374–386PubMedCrossRefGoogle Scholar
  8. 8.
    Starzl TE, Todo S, Tzakis A, Alessiani M, Casavilla A, Abu-Elmagd K, Fung J (1991) The many faces of multivisceral transplantation. Surg Gynecol Obst 172:335–344Google Scholar
  9. 9.
    Tzakis AG, Ricordi C, Alejandro R, Zeng Y, Fung J, Todo S, Demetris A, Mintz D, Starzl TE (1990) Pancreatic islet transplantation after abdominal exenteration and liver replacement. Lancet 336:402–405PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • N. Kobayashi
    • 1
  • K. Sakagami
    • 1
  • S. Takasu
    • 1
  • T. Matsuno
    • 1
  • K. Orita
    • 1
  1. 1.First Department of SurgeryOkayama University Medical SchoolOkayamaJapan

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