Cytokine Treatment of Radiation-Induced Hemopoietic Suppression: Results of Interleukin-6 and Granulocyte Colony-Stimulating Factor in a Murine Radiation Model
Following accidental radiation exposure or intensive radiotherapy severe hemopoietic depression often results from damage to hemopoietic stem and progenitor cell populations. The major risks of mortality following radiation exposure are sepsis (resulting from a lack of granulocytes) and hemorrhage (resulting from a lack of platelets). In recent years a variety of recombinant cytokines have been shown to possess hemopoietic activity [1, 2]. In particular, the ability of interleukin-6 (IL-6) to stimulate multilineage stem cell production [3–5] and the ability of granulocyte colony-stimulating factor (G-CSF) to enhance granulocyte production and function [6–8] have been demonstrated in vitro. Based on these effects, we hypothesized that in vivo IL-6 administration may amplify multilineage stem cell production, which if followed by G-CSF administration to induce multilineage cells towards granulocyte production, could provide an effective treatment for radiation- or drug-induced hemopoietic suppression. Before beginning such studies, however, it was necessary to evaluate the in vivo effects of each cytokine. The studies presented in this paper describe the ability of IL-6 and G-CSF to individually stimulate hemopoietic regeneration in vivo following radiation- induced hemopoietic injury in mice.
KeywordsIrradiate Mouse Cytokine Treatment Multipotent Progenitor Cell Hemopoietic Progenitor Cell Granulocyte Production
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- 4.Okano A, Suzuki C, Takatsuki F, Akiyama Y, Koike K, Ozawa K, Hirano T, Kishimoto T, Nakahata T, Asano S (1989) In vitro expansion of the murine pluripotent hemopoietic stem cell population in response to interleukin 3 and interleukin 6. Application to bone marrow transplantation. Transplantation 48:495PubMedCrossRefGoogle Scholar
- 7.Souza LM, Boone TC, Gabrilove J, Lai PH, Zsebo KM, Murdock DC, Chazin VR, Bruszewski J, Lu H, Chen KK, Barendt J, Platzer E, Moore MAS. Mertelsmann R, Weite K (1986) Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemia myeloid cells. Science 232:61PubMedCrossRefGoogle Scholar