An Adverse Wound Environment Reduces Leukocyte Phagocytosis and Protein Synthesis

  • B. R. Moelleken
  • S. J. Mathes
  • A. Amerhauser
  • D. C. Price
  • T. K. Hunt

Abstract

Little is known about the differences in behavior of leukocytes of specific types of wounds. It has been observed clinically that certain tissue types are considerably more able to resist bacterial inoculation, and some experimental evidence points to differences in leukocyte function. How is leukocyte activation affected by its immediate wound milieu, and what is it about a wound that causes a leukocyte to activate?

Keywords

Superoxide Sedimentation Acidity Barium NADPH 

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References

  1. 1.
    Moelleken BRW, Amerhauser A, Mathes SJ, Pytela R, Scheuenstuhl H, Breuss J, Hunt TK (1990) Adverse wound environments activate leukocytes prematurely. 10th annual meeting of the Surgical Infection Society, Cincinatti, OhioGoogle Scholar
  2. 2.
    Moelleken BRW, Amerhauser A, Mathes SJ, Pytela R, Scheuenstuhl H, Breuss J, Hunt TK (1990) Adverse wound environments activate leukocytes prematurely. 10th annual meeting of the Surgical Infection Society, Cincinatti, OhioGoogle Scholar
  3. 3.
    Zimmerli W, Seligmann, Gallin JI (1986) Exudation primes human and guinea pig neutrophils for subsequent responsiveness to the chemotactic peptide N-formylmethionyl-leucylphenylalanine and increases complement component Mac-1 receptor expression. J Clin Invest 77:925–933PubMedCrossRefGoogle Scholar
  4. 4.
    Altieri DC, Edgington TS (1988) A monoclonal antibody reacting with distinct adhesion molecules defines a transition in the functional state of the receptor CD11b/CD 18 (Mac-1). J Immunol 141(8):2656–2660PubMedGoogle Scholar
  5. 5.
    Moelleken BRW, Mathes SJ, Amerhauser A, Pytela R, Breuss J, Hunt TK (1991) An adverse wound environment activates leukocytes prematurely. Arch Surg 126(11):225–231PubMedGoogle Scholar
  6. 6.
    Wright DG, Gallin JI (1979) Secretory responses of human neutrophils: exocytosis of specific (secondary) granules by human neutrophils during adherence in vitro and during exudation in vivo. J Immunol 123:285–294PubMedGoogle Scholar
  7. 7.
    Mathes SJ, Nahai F (1982) Clinical applications for muscle and musculocutaneous flaps. St Louis, MosbyGoogle Scholar
  8. 8.
    Eshima I, Mathes SJ, Paty P (1990) Comparison of intracellular bacterial killing in leukocytes isolated from musculocutaneous and random pattern flaps. Plast Reconstr Surg 86(3):541–545PubMedCrossRefGoogle Scholar
  9. 9.
    Gottrup F, Firmin R, Hunt TK, Mathes SJ (1984) The dynamic properties of tissue oxygen in healing flaps. Surgery 95(5):527–536PubMedGoogle Scholar
  10. 10.
    Mathes SJ, Feng LJ, Hunt TK (1983) Coverage of the infected wound. Ann Surg 198(4):420–429PubMedCrossRefGoogle Scholar
  11. 11.
    Mathes SJ, Nahai F (1982) Clinical applications for muscle and musculocutaneous flaps. St Louis, MosbyGoogle Scholar
  12. 12.
    Mathes SJ, Alpert B, Chang N (1982) Use of muscle flap in chronic osteomyelitis: experimental and clinical correlation. Plast Reconstr Surg 69:815PubMedCrossRefGoogle Scholar
  13. 13.
    Hunt TK, Twomey P, Zederfeldt B, Dunphy JE (1967) Respiratory gas tensions and pH in healing wounds. Am J Surg 114:302–307PubMedCrossRefGoogle Scholar
  14. 14.
    Calderon W, Chang N, Mathes SJ (1986) Comparison of the effect of bacterial inoculation in musculocutaneous and fasciocutaneous flaps. Plast Reconstr Surg 77:785PubMedCrossRefGoogle Scholar
  15. 15.
    Eshima I, Mathes SJ, Paty P (1988) Comparison of the intracellular bacterial killing activity of the leukocyte in musculocutaneous and random pattern flaps. Proc Plast Surg Res Cncl, May 19–21, pp 49–51Google Scholar
  16. 16.
    Jonsson K, Brennan SS, Mathes SJ (1988) Tissue oxygen measurements in delayed skin flaps: a reconsideration of the mechanism of the delay phenomenon. Plast Reconstr Surg 82:328PubMedCrossRefGoogle Scholar
  17. 17.
    Jonsson K, Hunt TK, Mathes SJ (1988). Oxygen as an isolated variable influences resistance to infection. Ann Surg 208:783PubMedCrossRefGoogle Scholar
  18. 18.
    Gottrup FR, Hunt TK, Mathes SJ (1984) Dynamic properties of tissue oxygen in healing flaps. Surgery 95:527PubMedGoogle Scholar
  19. 19.
    Fyfe A, Holme ER, Zoma A, Whaley K (1987) C3b receptor (CR1) expression on the polymorphonuclear leukocytes from patients with systemic lupus erythematosus. Clin Exp Immunol 67(2):300–308PubMedGoogle Scholar
  20. 20.
    Loeffler DA, Keng PC, Baggs RB, Lord EM (1990) Lymphocytic infiltration and cytotoxicity under hypoxic conditions in the EMT6 mouse mammary tumor. Int J Cancer 45(3):462–467PubMedCrossRefGoogle Scholar
  21. 21.
    Gillespie MN, Kojima S, Owasoyo JO et al. (1987) Hypoxia provokes leukotriene-dependent neutrophil sequestration in perfused rabbit hearts. J Pharmacol Exp Ther 241(3):812–816PubMedGoogle Scholar
  22. 22.
    Pytela R (1988) Amino acid sequence of the murine Mac-1 a-1 chain reveals homology with the integrin family and an additional domain related to von Willebrand factor. EMBO J 7:1371–1378PubMedGoogle Scholar
  23. 23.
    Altieri DC, Morrissey JH, Edgington TS (1988) Adhesive receptor Mac-1 coordinates the activation of factor X on stimulated cells of monocytic and myeloid differentiation: an alternative initiation of the coagulation protease cascade. Proc Natl Acad Sci USA 85:7462–7466PubMedCrossRefGoogle Scholar
  24. 24.
    Pytela R, Pierschbacher MD, Ruoslahti E (1985) Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor. Cell 40:191–198PubMedCrossRefGoogle Scholar
  25. 25.
    Altieri DC, Bader R, Mannucci PM, Edgington TS (1988) Oligospecificity of the cellular adhesion receptor Mac-1 encompasses an inducible recognition specificity for fibrinogen. J Cell Biol 107:1893–1890PubMedCrossRefGoogle Scholar
  26. 26.
    Petrequin PR, Todd RF, Devali LJ, Boxer LA, Curnette JT III (1987) Association between gelatinase release and increased plasma membrane expression of the Mol glycoprotein. Blood 69(2):605–610PubMedGoogle Scholar
  27. 27.
    O’Shea JJ, Brown EJ, Seligmann BE, Metcalf JA, Frank MM, Gallin JI (1985) Evidence for distinct intracellular pools of receptors for C3b and Mac-1 in human neutrophils. J Immunol 74:1280–1290Google Scholar
  28. 28.
    Jack RM, Fearon DT (1988) Selective synthesis of mRNA and proteins by human peripheral blood neutrophils. J Immunol 140(12):4286–4293PubMedGoogle Scholar
  29. 29.
    Hughes V, Humphreys JM, Edwards SW (1987) Protein synthesis is activated in primed neutrophils: a possible role in inflammation. Biosci Rep 7(11):881–890PubMedCrossRefGoogle Scholar
  30. 30.
    Paty PB, Graeff R, Hunt TK Mathes SJ (1988) Biological priming of neutrophils in subcutaneous wounds. Arch Surg 123:1509PubMedCrossRefGoogle Scholar
  31. 31.
    Paty PB, Graeff RW, Mathes SJ, Hunt TK (1990) Superoxide production by wound neutrophils: evidence for increased activity of the NADPH oxidase. Arch Surg 125:65–69PubMedCrossRefGoogle Scholar
  32. 32.
    Zimmerli W, Lew PD, Cohen HJ, Waldvogel FA (1984) Comparative superoxide-generating system of neutrophils from blood and peritoneal exudates. Infect Immunol 46:625–630Google Scholar
  33. 33.
    Iwai A, Itoh M, Yokohama Y et al. (1989) Role of PAF in ischemia-reperfusion injury in the rat stomach. Scand J Gastroenterol [Suppl] 162:63–66CrossRefGoogle Scholar
  34. 34.
    Davis WB, Husney RM, Wewers MD et al. (1988) Effect of O2 partial pressure on the myeloperoxidase pathway of neutrophils. J Appl Physiol 65(5):1995–2003PubMedGoogle Scholar
  35. 35.
    Rotstein OD, Fiegel VDS, Simmons RL, Knighton DR (1988) The deleterious effect of reduced pH and hypoxia on neutrophil migration in vitro. J Surg Res 45(3):298–303PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag, Berlin Heidelberg 1993

Authors and Affiliations

  • B. R. Moelleken
    • 1
  • S. J. Mathes
    • 1
  • A. Amerhauser
    • 1
  • D. C. Price
    • 1
  • T. K. Hunt
    • 1
  1. 1.Department of SurgeryUniversity of CaliforniaSan FranciscoUSA

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