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Escherichia coli-Induced Septic Shock in the Baboon: An Animal Model for the Evaluation of Neutralising Monoclonal Antibodies to Phospholipase A2

  • R. Buchta
  • J. A. Green
  • G. M. Smith
  • C. Gabelish
  • D. Cairns
  • C. Salom
  • M. Conte
  • P. Mackie
  • R. Sublett
  • F. B. Taylor
  • I. A. Rajkovic
  • K. F. Scott

Abstract

Phospholipase A2 (PLA2) is a soluble enzyme which is present in both intra- and extracellular compartments (Van den Bosch 1980; Vadas and Pruzanski 1986). A secretory phospholipase A2 activity is clinically associated with onset and progression of sepsis and septic shock in humans (Vadas 1984; Vadas et al. 1988). We have identified this enzyme as a low molecular weight PLA2, identical to that associated with rheumatoid arthritis (Green et al. 1991). The enzyme is proinflammatory and when infused into the circulation of animals induces the haemodynamic effects characteristic of severe bacterial infection and septic shock (Vadas and Hay 1983). It is therefore a potential target for monoclonal antibody therapy directed towards later stages of sepsis and shock.

Keywords

Septic Shock PLA2 Activity Monoclonal Antibody Therapy Oklahoma Medical Research Foundation Tumour Necrosis Factor Elevation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag, Berlin Heidelberg 1993

Authors and Affiliations

  • R. Buchta
    • 1
  • J. A. Green
    • 1
  • G. M. Smith
    • 1
  • C. Gabelish
    • 1
  • D. Cairns
    • 1
  • C. Salom
    • 1
  • M. Conte
    • 1
  • P. Mackie
    • 1
  • R. Sublett
    • 2
  • F. B. Taylor
    • 3
  • I. A. Rajkovic
    • 1
  • K. F. Scott
    • 1
    • 4
  1. 1.Pacific Biotechnology Ltd.Rushcutters BayAustralia
  2. 2.R. W. Johnson Pharmaceutical Research InstituteSan DiegoUSA
  3. 3.Oklahoma Medical Research FoundationOklahoma CityUSA
  4. 4.Garvon Institute of Medical ResearchDarlinghurstAustralia

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