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Fluorescence Polarisation Immunoassay

  • Christian Klein
  • Hans-Georg Batz
  • Brigitte Draeger
  • Hans-Joachim Guder
  • Rupert Herrmann
  • Hans-Peter Josel
  • Ulrich Nägele
  • Roland Schenk
  • Bernd Vogt

Abstract

Since the introduction of radio immunoassay techniques [1], many different assay principles based on antibodies as specifiers have become routine tools in clinical diagnostics. Heterogeneous immunoassays, e.g. enzyme linked immunosorbant assays (ELISA) [2], require a series of time consuming incubation and wash steps, but they are characterized by a high sensitivity with a detection limit of 10−13 moll−1 and even lower.

Keywords

Human Serum Albumin Fluorescence Polarisation High Dynamic Range Effective Competition Fluorescence Polarisation Immunoassay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin HeidelBerg 1993

Authors and Affiliations

  • Christian Klein
    • 1
  • Hans-Georg Batz
    • 1
  • Brigitte Draeger
    • 1
  • Hans-Joachim Guder
    • 1
  • Rupert Herrmann
    • 1
  • Hans-Peter Josel
    • 1
  • Ulrich Nägele
    • 1
  • Roland Schenk
    • 1
  • Bernd Vogt
    • 1
  1. 1.Werk TutzingBoehringer Mannheim GmbHTutzingGermany

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