Advertisement

Enzyme Preparation for Optimal Extracorporeal Enzymatic Detoxification

  • M. Sawa
  • G. Brunner

Abstract

The clinical results of liver transplantation and extracorporeal liver perfusion in fulminant hepatic failure indicate that a whole liver is the best source for enzymatic detoxification. However, a human, monkey, or baboon liver are not always available for these therapeutic methods and at present cannot be preserved over long periods of time. The development of the lipophilic hollow fiber technique has made it possible to remove fat- soluble toxins from the blood and metabolize them by using extracted liver enzymes without complicated processes and immunologic reactions [1, 2; A. Freimann-Kersebaum, G. Brunner, unpublished observations]. Cellular or subcellular sources can be utilized as liver enzymes, and there are many differences in preparation, handling, and thus cost among them. In this study liver homogenate, isolated hepatocytes, microsomes or cytosol, and partially purified enzymes were investigated for the three detoxifying enzymes cytochrome P-450 hydroxylase (P-450-Hx), UDP-glucuronyltransferase (UDPGTase), and glutathione S-transferase (GSTase) with regard to total enzyme yield, efficacy, cost, storage, and stability.

Keywords

Enzyme Preparation Dodecanoic Acid Liver Homogenate Fulminant Hepatic Failure Nicotinamide Adenine Dinucleotide Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Brunner G, Tegtmeier F (1984) Enzymatic detoxification using lipophilic hollow- fiber membranes: I. Glucuronidation reactions. Artif Organs 8: 161–166PubMedCrossRefGoogle Scholar
  2. 2.
    Tsikas D, Brunner G (1989) Enzymatic detoxification using lipophilic hollow-fiber membranes: IV. Glutathione conjugation reactions. Int J Artif Organs 12: 121–128PubMedGoogle Scholar
  3. 3.
    Holloway CJ, Hussmann-Holloway S, Brunner G (1981) Assay of UDP- glucuronyltransferase with mixtures of acceptors using analytical capillary isotachophoresis. Electrophoresis 2: 25–31CrossRefGoogle Scholar
  4. 4.
    Seglen PO (1974) Preparation of rat liver cells. Exp Cell Res 74: 450–454CrossRefGoogle Scholar
  5. 5.
    Durst HD, Milano M, Kitta EJ, Connelly SA, Grushka E (1975) Phenacyl ester of fatty acids via crown ether catalyst for enhanced ultraviolet detection in liquid chromatography. Anal Chem 47: 1797–1801PubMedCrossRefGoogle Scholar
  6. 6.
    Sawa M, Tsikas D, Brunner G (1988) Glucuronide conjugation of phenols in isolated rabbit hepatocytes measured by high-pressure liquid chromatography. Chromatographia 26: 247–250CrossRefGoogle Scholar
  7. 7.
    Ellmann GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82: 70–77CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • M. Sawa
  • G. Brunner

There are no affiliations available

Personalised recommendations