Automation of DNA Profiling by Fluorescent Labelling of PCR Products

  • K. M. Sullivan
  • S. Pope
  • C. Kimpton
  • P. Gill
  • J. Sutton
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)

Abstract

Variable number tandem repeat (VNTR) and short tandem repeat (STR) loci display considerable variation within human populations and are useful markers for both the construction of human linkage maps (Nakamura et al. 1987) and for individual identification in forensic investigations (Hagelberg et al. 1991). PCR amplification of these loci has greatly improved the sensitivity of DNA profiling, and the amplified alleles from small VNTR loci such as D17S5, D1S80 and ApoB can be directly visualised and resolved on ethidium bromide-stained agarose or acrylamide gels (Hornet al. 1989, Kasai et al. 1990, Boerwinkle et al. 1989), whilst STRs are characterised on denaturing sequencing gels using sequence extension products as a size standard (Litt and Luty 1989; Weber and May 1989).

Keywords

Agarose Electrophoresis Ethidium dNTP Dinucleotide 

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References

  1. Boerwinkle E, Xiong W, Fourest E, Chan L (1989) Rapid typing of tandemly repeated hypervariable loci by the polymerase chain reaction: application to the apolipoprotein B 3′ hypervariable region. Proc Natl Acad Sci USA 86: 212–216PubMedCrossRefGoogle Scholar
  2. Hagelberg E, Gray IC, Jeffreys AJ (1991) Identification of the skeletal remains of a murder victim by DNA analysis. Nature 352: 427–429PubMedCrossRefGoogle Scholar
  3. Horn GT, Richards B, Klinger KW (1989) Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nucleic Acids Research 17: 2140PubMedCrossRefGoogle Scholar
  4. Kasai K, Nakamura Y, White R (1990) Amplification of a variable number tandem repeat (VNTR) locus (pMCT118) by the polymerase chain reaction (PCR) and its application to forensic science. J Forensic Sci 5: 1196–1200Google Scholar
  5. Litt M, Luty JA (1989) A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet 44: 397–401PubMedGoogle Scholar
  6. Mayrand PE, Robertson J, Ziegle J, Hoff LB, McBride LJ, Kronick MN Annales de Biologie Clinique (in press)Google Scholar
  7. Nakamura Y, Leppert M, O’Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, White R (1987) Variable number of tandem repeats (markers) for human gene mapping. Science 235: 1616–1622PubMedCrossRefGoogle Scholar
  8. Weber JL, May PE (1989) Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am J Hum Genet 44: 388–396PubMedGoogle Scholar
  9. Wu S, Seino S, Bell GI (1990) Human Collagen, type II, alpha 1, (COL2A1) gene: VNTR polymorphism detected by gene amplification. Nucl Acids Res 18: 3102PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • K. M. Sullivan
    • 1
  • S. Pope
    • 1
  • C. Kimpton
    • 1
  • P. Gill
    • 1
  • J. Sutton
    • 1
  1. 1.Central Research and Support EstablishmentThe Forensic Science ServiceAldermaston, Reading, BerkshireUK

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