Automation of DNA Profiling by Fluorescent Labelling of PCR Products
Variable number tandem repeat (VNTR) and short tandem repeat (STR) loci display considerable variation within human populations and are useful markers for both the construction of human linkage maps (Nakamura et al. 1987) and for individual identification in forensic investigations (Hagelberg et al. 1991). PCR amplification of these loci has greatly improved the sensitivity of DNA profiling, and the amplified alleles from small VNTR loci such as D17S5, D1S80 and ApoB can be directly visualised and resolved on ethidium bromide-stained agarose or acrylamide gels (Hornet al. 1989, Kasai et al. 1990, Boerwinkle et al. 1989), whilst STRs are characterised on denaturing sequencing gels using sequence extension products as a size standard (Litt and Luty 1989; Weber and May 1989).
KeywordsAgarose Electrophoresis Ethidium dNTP Dinucleotide
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