Advertisement

Paternity Analysis Using the Multilocus DNA Probe MZ 1.3

  • Peter M. Schneider
  • Rolf Fimmers
  • Ulrike Schacker
  • Christian Rittner
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)

Abstract

The multilocus minisatellite DNA probe MZ 1.3 detects hypervariable restriction fragment patterns in genomic DNA of man and animals. It can be used for segregation analysis in cases of disputed paternity (Schacker et al., 1991; Rittner et al., 1991a), for identification purposes in forensic medicine and stain analysis (Ogata et al., 1990; Rittner et al., 1991b), as well as in animal breeding for pedigree analysis and verification of inbred strains (Hins & Gruber, 1991). Hypervariable fragment patterns can be generated by using frequently cutting restriction enzymes, e.g. Hinf I, Hae Ill, Msp I, Mbo I, and Rsa I. A non-radioactive system using the digoxigenin antidigoxigenin system may be used for the detection of polymorphic fragments (B.E.S.T. Probe MZ 1.3, Biotest AG, Dreieich, FRG). Using this method, less than 1 µg of human genomic DNA can be detected (see Fig. 1). If sufficient genomic DNA is available for study, two parallel restriction enzyme digestions, e.g. using Hinf I and Hae Ill, should be carried out simultaneously as a control for the possible appearance of unassignable bands due to partially digested DNA (Schacker et al., 1991).

Keywords

Polymorphic Fragment Paternity Testing Putative Father Paternity Case Informative Band 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fimmers R, Schneider PM, Baur MP. Comparison of different methods for the calculation of indices of paternity (this volume)Google Scholar
  2. Hins J, Gruber FP. (1991) Genetisches Fingerprinting von lnzuchtlinien, Auszuchten, transgenen lndividuen und 3T3-Zellen von Mus musculus mit der Sonde B.E.S.T. MZ 1.3. J Vet Med 38: 61–72CrossRefGoogle Scholar
  3. Jeffreys AJ, M Turner, P Debenham. (1991) The efficiency of multi locus DNA fingerprint probes for individualization and establishment of family relationships, determined from extensive casework. Am J Hum Genet 48: 824–840PubMedGoogle Scholar
  4. Ogata M, Mattern R, Schneider PM, Schacker U, Kaufmann T, Rittner C. (1990) Quantitative and qualitative analysis of DNA extracted from postmortem muscle tissues. Z Rechtsmed 103: 397–406PubMedCrossRefGoogle Scholar
  5. Rittner C, U Schacker, PM Schneider. (1991) DNA fingerprinting as a tool of paternity testing in Germany. In: Berghaus G, B Brinkmann, C Rittner, M Staak (Hrg) “DNA technology and its forensic application”. Springer Heidelberg, 20–32CrossRefGoogle Scholar
  6. Rittner C, L Penzes, M Prager-Eberle, U Schacker, PM Schneider, U Jordan, V Schmidt, D Busse, HE Hildebrand, E Koops. (1991) DNA-Spurenanalyse. Kriminalistik 7: 439–442Google Scholar
  7. Rittner C, MP Baur, G Rittner, PM Schneider. (1991) Zum Beitrag des DNA-Gutachtens in Fällen mit verstorbenen Putativvätern (sog. Defizienz-Fälle). (submitted for publication)Google Scholar
  8. Schacker U, PM Schneider, B Holtkamp, E Bohnke, R Fimmers, HH Sonneborn, Rittner C. (1990) Isolation of DNA minisatellite probe MZ 1.3 and its application to DNA ‘fingerprinting’ analysis. For Sci lnt 44: 209–244Google Scholar
  9. Schacker U, T Kaufmann, PM Schneider, C Rittner. (1991) Reliability of restriction enzyme digestions of genomic DNA for the generation of DNA fingerprints. In: Berghaus G, B Brinkmann, C Rittner, M Staak (Hrg) “DNA technology and its forensic application”. Springer Verlag Heidelberg, 103–108CrossRefGoogle Scholar
  10. Schneider PM, Fimmers R, Bertrams J, Birkner P, Braunbeck K, Bulnheim U, Feuerbach M, Henke L, Iten E, Prinz M, Simeoni E, Rittner C. Biostatistical basis of individualization and segeration analysis using the multilocus DNA probe MZ 1.3: results of a collaborative study. (submitted for publication)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Peter M. Schneider
    • 1
  • Rolf Fimmers
    • 2
  • Ulrike Schacker
    • 1
  • Christian Rittner
    • 1
  1. 1.Institut für RechtsmedizinJohannes Gutenberg-Universität MainzMainzGermany
  2. 2.Institut für Medizinische StatistikUniversitätsklinik BonnGermany

Personalised recommendations