Analysis of D1S80 (pMCT 118) Locus Polymorphism in an Italian Population Sample by the Polymerase Chain Reaction

  • A. Tagliabracci
  • R. Giorgetti
  • A. Agostini
  • M. Cingolani
  • S. D. Ferrara
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)

Abstract

The D1S80 locus, identified by the probe pMCT118, is suitable for forensic haemogenetic purposes because of its wide polymorphism, the high rate of heterozygosity and the easiness and speed of the analysis method (Nakamura et al. 1988; Kasai et al.1990; Budowle et al. 1991).

Keywords

Glycerol Bromide Electrophoresis Polyacrylamide Ethidium 

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References

  1. Budowle B, Baechtel FS (1990) Modifications to improve the effectiveness of restriction fragment length polymorphism typing. Appl Theor Electrophoresis, 1: 181–187Google Scholar
  2. Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the VNTR Locus D1S80 by the PCR Followed by High-Resolution PAGE. Am J Hum Genet, 48: 137–144PubMedGoogle Scholar
  3. Elder JK, Southern EM (1983) Measurement of DNA Length by Gel Electrophoresis II: Comparison of Methods for Relating Mobility to Fragment Length. Anal Biochem, 128: 227–231PubMedCrossRefGoogle Scholar
  4. Kasai K, Nakamura Y, White R (1990) Amplification of Variable Number of Tandem Repeats (VNTR) Locus (pMCT 118) by the Polymerase Chain Reaction (PCR) and Its Application to Forensic Science. J For Sci, 35: 1196–1200Google Scholar
  5. Nakamura Y, Carlson M, Krapcho K, White R (1988) Isolation and Mapping of a Polymorphic DNA Sequence (pMCT118) on Chromosome 1p (D1S80). Nucleic Acids Res, 16: 9364PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • A. Tagliabracci
    • 1
  • R. Giorgetti
    • 1
  • A. Agostini
    • 1
  • M. Cingolani
    • 1
  • S. D. Ferrara
    • 1
  1. 1.Istituto di Medicina Legale dell’Università di Ancona, PoliclinicoTorretteItaly

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