DNA typing from formalin-fixed, paraffin-embedded tissues

  • H. Fukushima
  • M. Ota
  • K. Tanaka
  • A. Ichinose
  • K. Sato
  • K. Honda
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)

Abstract

Serologically defined individual variation has been shown to be considerable in HLA class I and class II. Recently, HLA alleles were defined using polymerase chain reaction(PCR-DNA typing). As previously reported(Ota 1991), the PeR-restriction fragment length polymorphism(PCR-RFLP) method is the most useful to define the HLA-DQA1, DQB1, DRB1 and DPB1 alleles. The advantage of this method is the use of some informative restriction enzymes, which have either a single cleavage site or alternatively no cleavage site in the amplified DNA region.

Keywords

Formalin Phenol EDTA Bromide Electrophoresis 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • H. Fukushima
    • 1
  • M. Ota
    • 1
  • K. Tanaka
    • 2
  • A. Ichinose
    • 2
  • K. Sato
    • 1
  • K. Honda
    • 1
  1. 1.Department of Legal MedicineShinshu University School of MedicineMatsumoto, Nagano 390Japan
  2. 2.Department of AnesthesiologyShinshu University School of MedicineMatsumoto, Nagano 390Japan

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