Investigations on the Forensic application of 4 AMPFLP systems

  • P. Wiegand
  • S. Rand
  • B. Brinkmann
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)


One major problem with stain examinations is that the amount of biological material is often too small and the extracted DNA is highly degraded due to the environmental conditions. It is therefore highly probable that only PCR can be successfully used for DNA analysis and individualisation.


Hair Shaft Vaginal Swab Forensic Application Rape Case Cigarette Butt 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Akane A, Shiono H, Matsubara K, Nakahori Y, Seki S, Nagafuchi S, Yamada M, Nakagome Y (1991) Sex identification of forensic specimens by polymerase chain reaction (PCR): two alternative methods. For Sci Int 49: 81–88Google Scholar
  2. Allen RC, Graves G, Budowle B (1989) Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Bio Techniques 7: 736–744CrossRefGoogle Scholar
  3. Boerwinkle E, Xiong W, Fourest E, Chang L (1989) Rapid typing of tandemly repeated hypervariable loci by the polymerase chain reaction: application to the apolipoprotein B 3′ hypervariable region. Proc Natl Acad Sci USA 86: 212–216PubMedCrossRefGoogle Scholar
  4. Brinkmann B, Rand S, Wiegand P (1991) Population and family data of RFLP’s using selected single- and multi-locus systems. Int J Leg Med 104: 81–86CrossRefGoogle Scholar
  5. Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the variable vumber of tandem repeats locus D1S80 by the polymerase chain reaction followed by high resolution polyacrylamide gel electrophoresis. Am J Hum Genet 48: 137–144PubMedGoogle Scholar
  6. Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic applications of DNA “fingerprints”. Nature 318: 577–579PubMedCrossRefGoogle Scholar
  7. Higuchi R (1989) Sinple and rapid preparation of samples for PCR. In: Erlich, HA (ed) PCR technology principles and applications for DNA amplification. Stockton Press, New York London Tokyo Melbourne Hong Kong pp 31–39Google Scholar
  8. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10 (4): 506–513PubMedGoogle Scholar
  9. Wiegand P, Schtirenkamp M, Schütte U (1991 in press) DNA extraction from mixtures of body fluid using mild preferential lysis. Int J Leg MedGoogle Scholar
  10. Wolff RK, Nakamura YR, White R (1988) Molecular characterization of a spontaneously generated new allele at a VNTR locus: no exchange of flanking DNA sequence. Genomics 3: 347–351PubMedCrossRefGoogle Scholar
  11. Wu S, Seino S, Bell GI (1990) Human collagen, type II, alpha 21, (COL2A1) gene: VNTR polymorphism detected by gene amplification, Nucleic Acids Res 18: 3102PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • P. Wiegand
    • 1
  • S. Rand
    • 1
  • B. Brinkmann
    • 1
  1. 1.Institut für RechtsmedizinWestfälische-Wilhelms-UniversitätMünsterGermany

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