ABO Blood Grouping and Species Identification of Bloodstains by Sandwich ELISA Using Monoclonal Antibody Specific for Human Erythrocyte Band 3
Species identification and ABO blood grouping of bloodstains constitute the main subjects in medicolegal practices. The methods employed for species identification and ABO blood grouping, e.g. immunodiffusion with anti-human hemoglobin antibody and absorption-elution test using anti-ABO blood group sera, are well established and highly reliable. However, methods of distinguish between species, especially between humans and other primates, and methods of ABO blood grouping of bloodstains contaminated by other body fluids are not yet satisfactory. In our study of species-specific epitopes on human erythrocyte membrane, we produced monoclonal antibodies (mAbs) to species specific-epitopes on human erythrocyte membrane band 3. One (P3–9H) of these mAbs could even discriminate erythrocytes of human from those of chimpanzee (Kimura 1990). Since erythrocyte membrane band 3 carries ABO blood group active carbohydrate chains, the use of P3-9H may be applicable not only to human blood identification but also to ABO blood grouping.
KeywordsCarbohydrate EDTA Electrophoresis Gelatin Asparagine
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