Skip to main content
SpringerLink
Log in

Spin-Trapping in Biological Systems

  • Conference paper
Electron Spin Resonance (ESR) Applications in Organic and Bioorganic Materials

  • 105 Accesses

Abstract

Spin trapping is a technique for detecting short-lived (reactive) free radicals. It consists in producing an ESR detectable (long-lived) radical by an addition reaction of the short-lived radical on a trapping agent. The spin trap is generally a nitrone or a nitroso compound. then the spin adduct an aminoxyl radical having at least a three lines spectrum. Nuclei having a magnetic moment can interact with the aminoxyl unpaired electron causing further hyperfine splitting whose constants can help to identify the trapped species.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliography

  1. De Groot, J. J. C., Garsen, G. J., Veldink, G. A., Vliegenthart, J. F. G. and Bolding, J., (1973) Biochim. Biophys. Acta, 326, 279–284.

    Google Scholar 

  2. Janzen, E, G., (1980) in Free Radicals in Biologiy, Vol IV (Pryor, W. A., Ed.) Academic Press, pp 115-154.

    Google Scholar 

  3. Mason, R. P., (1984) in Spin Labelling in Pharmacology, Academic Press, pp 87-129.

    Google Scholar 

  4. Pou, S., Hassett, D. J., Britigan, B. E., Cohen, M. S. and Rosen, G. M., (1989) Anal. Biochem., 177, 1–6.

    Article  CAS  Google Scholar 

  5. Bombard, S., Thesis, Université René Descartes Paris (1990).

    Google Scholar 

  6. Bombard, S., Chopard, C., Nam, N. H. and Chottard, J-C., (in preparation).

    Google Scholar 

  7. Buettner, G. R., (1987), Free Rad. Biol. Med., 3, 259–303.

    Article  CAS  Google Scholar 

  8. Perkins, M. J., (1980), in Adv. Phys. Org. Chem., Vol. 7, pp 1–64.

    Google Scholar 

  9. Li, A. S. W., Cummings, K. B., Roethling, H. P., Buettner, G. R. and Chignell, C. F., (1988) J. Mag. Res., 79, 140–142.

    Article  Google Scholar 

  10. Dubose, C. M., Rehorek, D., Oehler, U. M. and Janzen, E. G., (1988), Free Rod. Biol. Med., 5, 55–56.

    Article  CAS  Google Scholar 

  11. Samuelsson, B., (1983) Science, 220, 568–575.

    Article  CAS  Google Scholar 

  12. Samuelsson, B., Dahlen, S. E., Lindgren, J. A., Rouzer, C. A. & Serhan, C. N., (1987) Science, 237, 1171–1176.

    Article  CAS  Google Scholar 

  13. Galliard, T. & Chan, H. W. S., (1980) The Biochemistry of Plants, Vol.4, Academic Press, New York, pp 131–160.

    Google Scholar 

  14. Vliegenthart, J. F. G. & Veldink, G. A. (1982) in Free Radicals in Biology, Vol V, (Pryor, W. A., Ed.), Academic Press, New York, pp 29–64.

    Google Scholar 

  15. De Groot, J. J. C., Veldink, G. A., Vliegenthart, J. F. G., Bolding, J., Wever, R. & Van Gelder, B. F., (1975) Biochim. Biophys. Acta, 377, 71–79.

    Google Scholar 

  16. Egmond, M. R., Brunori, M. & Fasella, P. M., (1976) Eur. J. Biochem., 61, 93–100.

    Article  CAS  Google Scholar 

  17. Lands, W. E. M., (1984) Prostaglandins, Leukotrienes Med., 13, 35–46.

    Article  CAS  Google Scholar 

  18. Ludwig, P., Holzhütter, H., Colosimo, A., Silvestrini, M. C., Schewe, T. & Rapoport S. M., (1987) Eur. J. Biochem., 168, 325–337.

    Article  CAS  Google Scholar 

  19. Hatzelmann, A., Schatz, M. & Ullrich, V. (1989) Eur. J. Biochem., 180, 527–533.

    Article  CAS  Google Scholar 

  20. Clapp, C. H., Banerjee, A. & Rotenberg, S. A. (1985) Biochemistry, 24, 1826–1830.

    Article  CAS  Google Scholar 

  21. Kemal, C., Louis-Flamberg, P., Krupinski-Olsen, R. & Shorter, A. L. (1987) Biochemistry, 26, 7064–7072.

    Article  CAS  Google Scholar 

  22. Mansuy, D., Cucurou, C., Biatry, B. & Battioni, J. P. (1988) Biochem. Biophys. Res. Commun., 151, 339–346.

    Article  CAS  Google Scholar 

  23. Kulkarni, A. P. & Cook, D. C. (1988) Res. Commun. Chem. Pathol. Pharm., 61, 305–314.

    CAS  Google Scholar 

  24. Galey, J. B., Bombard, S., Chopard, C., Girerd, J. J., Lederer, F., Do-Cao-Thang, Nguyen-Hoang-Nam, Mansuy, D. & Chottard, J.C. (1988) Biochemistry, 27, 1058–1066.

    Article  CAS  Google Scholar 

  25. Hill, H. A. O & Thornalley, P. J. (1981) FEBS Lett., 125, 235–238.

    Article  CAS  Google Scholar 

  26. Huang, P. C. & Kosower E. M. (1968) J. Am. Chem. Soc., 90, 2354–2361.

    Article  CAS  Google Scholar 

  27. Yao, H. C. & Resnick, P. (1965) J. Org. Chem., 30, 2832–2834.

    Article  CAS  Google Scholar 

  28. Hardie, R. L. & Thomson, R. H. (1957) J. Chem. Soc., 2512-2518.

    Google Scholar 

  29. Rosen, G. M. & Rauckman, E. J. (1980) Mol. Pharmacol., 17, 233–238.

    CAS  Google Scholar 

  30. Davies, M. J., (1988) Biochim. Biophys. Acta, 964, 28–35.

    Article  CAS  Google Scholar 

  31. Smith, W. L. & Lands, W. E. M., (1972) J. Biol. Chem., 247, 1038–1047.

    CAS  Google Scholar 

  32. Kühn, R., Stender, H., Schewe, T., Lankin, V. Z., Nekrasov, A. & Rapoport, S. M., (1986) FEBS, 203, 247–252.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Unité associée au CNRS, (URA 400), Université René Descartes, 45 rue des Saints-Pères, 75270, Paris Cedex 06, France

    Claude Chopard

Authors
  1. Claude Chopard
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Groupe d’Application de la Resonance Paramagnétique Electronique, I. T. F. Lyon, Avenue Guy de Collongue, B.P. 60, F-69132, Ecully Cedex, France

    B. Catoire

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Chopard, C. (1992). Spin-Trapping in Biological Systems. In: Catoire, B. (eds) Electron Spin Resonance (ESR) Applications in Organic and Bioorganic Materials. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77214-6_9

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-77214-6_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77216-0

  • Online ISBN: 978-3-642-77214-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation