Spin-Trapping in Biological Systems

  • Claude Chopard
Conference paper

Abstract

Spin trapping is a technique for detecting short-lived (reactive) free radicals. It consists in producing an ESR detectable (long-lived) radical by an addition reaction of the short-lived radical on a trapping agent. The spin trap is generally a nitrone or a nitroso compound. then the spin adduct an aminoxyl radical having at least a three lines spectrum. Nuclei having a magnetic moment can interact with the aminoxyl unpaired electron causing further hyperfine splitting whose constants can help to identify the trapped species.

Keywords

Toluene Adduct DPPH Hydroperoxide Rosen 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliography

  1. 1.
    De Groot, J. J. C., Garsen, G. J., Veldink, G. A., Vliegenthart, J. F. G. and Bolding, J., (1973) Biochim. Biophys. Acta, 326, 279–284.Google Scholar
  2. 2.
    Janzen, E, G., (1980) in Free Radicals in Biologiy, Vol IV (Pryor, W. A., Ed.) Academic Press, pp 115-154.Google Scholar
  3. 3.
    Mason, R. P., (1984) in Spin Labelling in Pharmacology, Academic Press, pp 87-129.Google Scholar
  4. 4.
    Pou, S., Hassett, D. J., Britigan, B. E., Cohen, M. S. and Rosen, G. M., (1989) Anal. Biochem., 177, 1–6.CrossRefGoogle Scholar
  5. 5.
    Bombard, S., Thesis, Université René Descartes Paris (1990).Google Scholar
  6. 6.
    Bombard, S., Chopard, C., Nam, N. H. and Chottard, J-C., (in preparation).Google Scholar
  7. 7.
    Buettner, G. R., (1987), Free Rad. Biol. Med., 3, 259–303.CrossRefGoogle Scholar
  8. 8.
    Perkins, M. J., (1980), in Adv. Phys. Org. Chem., Vol. 7, pp 1–64.Google Scholar
  9. 9.
    Li, A. S. W., Cummings, K. B., Roethling, H. P., Buettner, G. R. and Chignell, C. F., (1988) J. Mag. Res., 79, 140–142.CrossRefGoogle Scholar
  10. 10.
    Dubose, C. M., Rehorek, D., Oehler, U. M. and Janzen, E. G., (1988), Free Rod. Biol. Med., 5, 55–56.CrossRefGoogle Scholar
  11. 11.
    Samuelsson, B., (1983) Science, 220, 568–575.CrossRefGoogle Scholar
  12. 12.
    Samuelsson, B., Dahlen, S. E., Lindgren, J. A., Rouzer, C. A. & Serhan, C. N., (1987) Science, 237, 1171–1176.CrossRefGoogle Scholar
  13. 13.
    Galliard, T. & Chan, H. W. S., (1980) The Biochemistry of Plants, Vol.4, Academic Press, New York, pp 131–160.Google Scholar
  14. 14.
    Vliegenthart, J. F. G. & Veldink, G. A. (1982) in Free Radicals in Biology, Vol V, (Pryor, W. A., Ed.), Academic Press, New York, pp 29–64.Google Scholar
  15. 15.
    De Groot, J. J. C., Veldink, G. A., Vliegenthart, J. F. G., Bolding, J., Wever, R. & Van Gelder, B. F., (1975) Biochim. Biophys. Acta, 377, 71–79.Google Scholar
  16. 16.
    Egmond, M. R., Brunori, M. & Fasella, P. M., (1976) Eur. J. Biochem., 61, 93–100.CrossRefGoogle Scholar
  17. 17.
    Lands, W. E. M., (1984) Prostaglandins, Leukotrienes Med., 13, 35–46.CrossRefGoogle Scholar
  18. 18.
    Ludwig, P., Holzhütter, H., Colosimo, A., Silvestrini, M. C., Schewe, T. & Rapoport S. M., (1987) Eur. J. Biochem., 168, 325–337.CrossRefGoogle Scholar
  19. 19.
    Hatzelmann, A., Schatz, M. & Ullrich, V. (1989) Eur. J. Biochem., 180, 527–533.CrossRefGoogle Scholar
  20. 20.
    Clapp, C. H., Banerjee, A. & Rotenberg, S. A. (1985) Biochemistry, 24, 1826–1830.CrossRefGoogle Scholar
  21. 21.
    Kemal, C., Louis-Flamberg, P., Krupinski-Olsen, R. & Shorter, A. L. (1987) Biochemistry, 26, 7064–7072.CrossRefGoogle Scholar
  22. 22.
    Mansuy, D., Cucurou, C., Biatry, B. & Battioni, J. P. (1988) Biochem. Biophys. Res. Commun., 151, 339–346.CrossRefGoogle Scholar
  23. 23.
    Kulkarni, A. P. & Cook, D. C. (1988) Res. Commun. Chem. Pathol. Pharm., 61, 305–314.Google Scholar
  24. 24.
    Galey, J. B., Bombard, S., Chopard, C., Girerd, J. J., Lederer, F., Do-Cao-Thang, Nguyen-Hoang-Nam, Mansuy, D. & Chottard, J.C. (1988) Biochemistry, 27, 1058–1066.CrossRefGoogle Scholar
  25. 25.
    Hill, H. A. O & Thornalley, P. J. (1981) FEBS Lett., 125, 235–238.CrossRefGoogle Scholar
  26. 26.
    Huang, P. C. & Kosower E. M. (1968) J. Am. Chem. Soc., 90, 2354–2361.CrossRefGoogle Scholar
  27. 27.
    Yao, H. C. & Resnick, P. (1965) J. Org. Chem., 30, 2832–2834.CrossRefGoogle Scholar
  28. 28.
    Hardie, R. L. & Thomson, R. H. (1957) J. Chem. Soc., 2512-2518.Google Scholar
  29. 29.
    Rosen, G. M. & Rauckman, E. J. (1980) Mol. Pharmacol., 17, 233–238.Google Scholar
  30. 30.
    Davies, M. J., (1988) Biochim. Biophys. Acta, 964, 28–35.CrossRefGoogle Scholar
  31. 31.
    Smith, W. L. & Lands, W. E. M., (1972) J. Biol. Chem., 247, 1038–1047.Google Scholar
  32. 32.
    Kühn, R., Stender, H., Schewe, T., Lankin, V. Z., Nekrasov, A. & Rapoport, S. M., (1986) FEBS, 203, 247–252.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Claude Chopard
    • 1
  1. 1.Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Unité associée au CNRS(URA 400), Université René DescartesParis Cedex 06France

Personalised recommendations