Skip to main content
SpringerLink
Log in

Inhalation Studies with Airborne Particulates in Rodents: Cytotoxic and Genotoxic Effects on Alveolar Macrophages and Bone Marrow Cells

  • Conference paper
Book cover Advances in Controlled Clinical Inhalation Studies

Part of the book series: ILSI Monographs ((ILSI MONOGRAPHS))

  • 56 Accesses

Abstract

Industrialization, traffic, and urbanization are the most important factors causing contamination of the atmosphere with particulate and gaseous pollutants. These represent a very complex chemical mixture composed of several hundreds of mostly organic compounds (Helmes et al. 1982; Schlipkoter 1983). In previous studies it has been repeatedly reported that organic extracts are cytotoxic, mutagenic, and carcinogenic in a number of short-term bioassays using rodent and human tissue culture cells (Hadnagy et al. 1986, 1989; Motykiewiecz et al. 1991; Seemayer et al. 1984, 1988, 1989). Airborne particulates with a diameter of less than 5 μm are of special importance as they can reach the bronchoalveolar space in human lung by respiration. Macrophages in alveoli come into direct contact with noxious particles and gases as well as with diverse pathogenic microorganisms.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Beck B, Brain JD, Wolfthal SF (1988) Assessment of lung injury produced by particulate emissions of space heaters burning automotive waste oil. Ann Occup Hyg 32:257–265

    Google Scholar 

  • Behrendt H, Seemayer NH, Tomingas R (1986) Evidence for specific ultrastructural alterations of macrophages induced by airborne particulates. AEROSOL: Formation and reactivity. 2nd international aerosol conference, Berlin. Pergamon, New YorkA, pp 212–214

    Google Scholar 

  • Behrendt H, Seemayer NH, Holle A, Dehnen W (1987a) Effect of extract of airborne particulates and of Ca++-ionophore A 23187 on formation and release of surfactant from human type II pneumocytes. J Aerosol Sci 18:705–708

    Article  CAS  Google Scholar 

  • Behrendt H, Seemayer NH, Braumann A, Nissen M (1987b) Electron microscopy investigations on the effect of quartz dust DQ 12 on human monocyte/ macrophages in vitro. Silicosis report North-Rhine Westfalia, vol 16. Steinkohlebergbauverein, Essen, pp 171–183

    Google Scholar 

  • Behrendt H, Seemayer NH, Happel A, Tomingas R (1990) Dust induced alterations of human macrophages. In: Seemayer NH, Hadnagy W (eds) Environmental hygiene II. Springer, Berlin Heidelberg New York, pp 195–198

    Google Scholar 

  • Brain JD (1986) Toxicological aspects of alterations of pulmonary macrophage function. Annu Rev Pharmacol Toxicol 26:547–565

    Article  PubMed  CAS  Google Scholar 

  • Carnow BW (1978) The “urban factor” and lung cancer: cigarette smoking or air pollution. Environ Health Perspect 22:17–21

    Article  PubMed  CAS  Google Scholar 

  • Castranova V, Bowman L, Reasor MJ et al. (1985) The response of rat alveolar macrophages to chronic inhalation of coal dust and/or diesel exhaust. Environ Res 36:405–419

    Article  PubMed  CAS  Google Scholar 

  • Dannenberg AM (1977) Influence of environmental factors on the respiratory tract. J Reticuloendothel Soc 22:273–289

    PubMed  Google Scholar 

  • Doll R (1978) Atmospheric pollution and lung cancer. Environ Health Perspect 22:23–31

    Article  PubMed  CAS  Google Scholar 

  • Gardner DE (1984) Alterations in macrophage functions by environmental chemicals. Environ Health Persp 55:343–358

    Article  CAS  Google Scholar 

  • Green GM, Jakab GJ, Low RB, Davis GS (1977) Defense mechanisms of the respiratory membrane. Am Rev Respir Dis 115:479–514

    PubMed  CAS  Google Scholar 

  • Gulyas H, Labedzka M, Geertz R, Gercken G (1990) Alveolar macrophage damage by dusts in vitro is correlated with arsenic, lead and antimony contents. In: Seemayer NH, Hadnagy W (eds) Environmental hygiene II. Springer, Berlin Heidelberg New York, pp 191–194

    Google Scholar 

  • Hadnagy W, Seemayer NH, Tomingas R (1986) Cytogenetic effects of airborne particulate matter in human lymphocytes in vitro. Mutat Res 175:97–101

    Article  PubMed  CAS  Google Scholar 

  • Hadnagy W, Seemayer NH, Tomingas R, Ivanfy K (1989) Comparative study of sister-chromatid exchanges and chromosomal aberrations induced by airborne particulates from an urban and a highly industrialized location in human lymphocyte cultures. Mutat Res 225:27–32

    Article  PubMed  CAS  Google Scholar 

  • Hatch GE, Boykin E, Graham JA, Lewtas J, Pott F, Loud K, Mumforce, JL (1985) Inhalable particles and pulmonary host defense: in vivo and in vitro effects of ambient air and combustion particles. Environ Res 36:67–80

    Article  PubMed  CAS  Google Scholar 

  • Helmes CT, Atkinson DL, Jaffer J, Sigman CC, Thompson KL, Kelsey MI, Kraybill HF, Munn JI (1982) Evaluation and classification of the potential carcinogenicity of organic air pollution. J Environ Sci Health A17:321–389

    Article  CAS  Google Scholar 

  • Lave LB, Seskin EP (1970) Air pollution and human health. Science 169:723–733

    Article  PubMed  CAS  Google Scholar 

  • Lippmann M, Albert R, Yeates D (1979) Effects of inhaled particles on human and animals: deposition, retention and clearance. In: Air particles. National Research Council. University Park Press, Baltimore, pp 107–145

    Google Scholar 

  • Liu WK, Tarn NFY, Wong MH, Cheung YH (1987) Cytotoxicity of airborne particles from roadside urban gardens. Sci Total Environ 59:267–276

    Article  PubMed  CAS  Google Scholar 

  • Loose LD, Silkworth JB, Charbonneau T, Blumenstock F (1981) Environmental chemical-induced macrophage dysfunction. Environ Health Perspect 39:79–82

    Article  PubMed  CAS  Google Scholar 

  • Motykiewicz G, Hadnagy W, Seemayer NH, Szeliga J, Tkocz A, Chorazy M (1991) Influence of airborne suspended matter on mitotic cell division. Mut at Res 260:195–202

    Article  PubMed  CAS  Google Scholar 

  • Pascoe S, Gatehouse D (1986) The use of a simple haematoxylin and eosin staining procedure to demonstrate micronuclei within rodent bone marrow. Mutat Res 164:237–243

    PubMed  CAS  Google Scholar 

  • Odagiri Y, Adachi S, Katayama H, Takemoto K (1986) Detection of the cytogenetic effect of inhaled aerosols by the micronucleus test. Mutat Res 170:79–83

    Article  PubMed  CAS  Google Scholar 

  • Repace JL (1982) Indoor air pollution. Environ Int 8:21–36

    Article  CAS  Google Scholar 

  • Ruiz F, Videla LA, Parra MA, Trier A, Silvia C (1988) Air pollution impact on phagocytic capacity of peripheral blood macrophages and antioxidant activity of plasma among school children. Arch Environ Health 43:286–291

    PubMed  CAS  Google Scholar 

  • Schlipköter H-W (1983) Lufthygienische Probleme der Großstadt. Arcus 5:244–250

    Google Scholar 

  • Seemayer NH, Manojlovic N (1980) Cytotoxic effects of air pollutants on mammalian cells in vitro. Toxicology 17:177–182

    Article  PubMed  CAS  Google Scholar 

  • Seemayer NH, Manojlovic N, Schürer C-C, Tomingas R (1984) Cell cultures as a tool for detection of cytotoxic, mutagenic and carcinogenic activity of airborne particulate matter. J Aerosol Sci 15:426–430

    Article  CAS  Google Scholar 

  • Seemayer NH, Manojlovic N, König H, Tomingas R (1988) Comparative investigation of carcinogenic and mutagenic activity of airborne particulate matter from polluted areas using human and rodent tissue culture cells. Ann Occup Hyg 32:247–256

    Google Scholar 

  • Seemayer NH, Hadnagy W, Tomingas R (1989) Assessment of health risks by air pollutants from in vitro cytotoxicity and genotoxicity testing on mammalian cells: a longitudinal study from 1975 until now. In: Brasser LJ, Mulder WC (eds) Man and his ecosystem, vol 2. Proceedings of the 8th world clean air congress 1989, The Hague, The Netherlands, 11–15 September 1989. Elsevier, Amsterdam, pp 137–142

    Google Scholar 

  • Seemayer NH, Happel A, Behrendt H, Hadnagy W, Tomingas R (1990) Comparison of cytotoxicity of airborne particulates to rat and human macrophages. J Aerosol Sci 21:387–391

    Article  Google Scholar 

  • Sugiri D, Behrendt H, Seemayer NH (1985) Biological effects of city smog extract: IX. Quantitative cytological investigation on the cytotoxic effect of city smog extracts on macrophages in vitro. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt I Orig Reihe B 181:226–239

    CAS  Google Scholar 

  • Sweeny TD, Brain JD, Godlewski JJ (1988) Preexisting disease. In: Brain JD et al. (eds) Variation in susceptibility to inhaled pollutants. John Hopkins Press, Baltimore, pp 142–158

    Google Scholar 

  • Tomatis L (ed) (1990) Air pollution and human cancer. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Van Houdt JJ, Rietjens IMCM (1988) Toxicity of airborne particulate matter to rat alveolar macrophages: a comparative study of five extracts collected indoors and outdoors. Toxical In Vitro 2:121–123

    Article  Google Scholar 

  • World Health Organization (1978) Environmental health criteria: 6. Principles and methods for evaluating toxicity of chemicals, part 1. WHO, Geneva

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medizinisches Institut f. Umwelthygiene, Heinrich-Heine-Universität, Gurlittstraße 53, 4000, Düsseldorf 1, Germany

    A. Kiell, W. Hadnagy, N. H. Seemayer, H. Behrendt & R. Tomingas

Authors
  1. A. Kiell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Hadnagy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. H. Seemayer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Behrendt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Tomingas
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Experimentelle Pathologie, Medizinische Hochschule Hannover, Konstanty-Gutschow-Straße 8, 3000, Hannover 61, Germany

    Ulrich Mohr Prof. Dr. med.

  2. Dept. Health and Epidemiology, University of British Columbia, 5804 Fairview Crescent, Mather Building, V6T 1W5, Vancouver, BC, Canada

    David V. Bates M.D., F.R.C.P., F.R.C.P.C., F.A.C.P., F.R.S.C.

  3. Abteilung Pneumologie, Medizinische Hochschule Hannover, Konstanty-Gutschow-Straße 8, 3000, Hannover 61, Germany

    H. Fabel Prof. Dr. med.

  4. Pulmonary and Critical Care Unit, University of Rochester Medical Center, 601 Elmwood Avenue, 14642, Rochester, NY, USA

    Mark J. Utell M.D. (Professor of Medicine and Environmental Medicine) (Professor of Medicine and Environmental Medicine)

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Kiell, A., Hadnagy, W., Seemayer, N.H., Behrendt, H., Tomingas, R. (1993). Inhalation Studies with Airborne Particulates in Rodents: Cytotoxic and Genotoxic Effects on Alveolar Macrophages and Bone Marrow Cells. In: Mohr, U., Bates, D.V., Fabel, H., Utell, M.J. (eds) Advances in Controlled Clinical Inhalation Studies. ILSI Monographs. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77176-7_30

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-77176-7_30

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77178-1

  • Online ISBN: 978-3-642-77176-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation