Ischemic Neuronal Injury Modified by Basic Fibroblast Growth Factor
Basic fibroblast growth factor (bFGF) is one of the growth factors for neurons, i. e., neurotrophic factors. We hereby report that bFGF is effective for recovery of neurons from ischemic injury in vitro and in vivo. Cortical, thalamic, and hippocampal neurons were cultured from 18-day-old rat embryo, and bFGF was added to the serumfree culture medium. bFGF supported the survival of hippocampal neurons in vitro in a dose-dependent manner. Cortical and thalamic neurons were also supported in their survival by bFGF, though the effect was less significant than in hippocampal neurons. bFGF was effective in vivo for the prevention of retrograde degeneration of thalamic neurons caused by cortical infarction. bFGF (lng/0. lml) was injected intracisternally once a week for four times starting 1 day after occlusion. The thalamic degeneration was significantly reduced by bFGF when compared with the vehicle-injected group. bFGF induced a glial reaction in the brain tissue close to the ventriculocisternal systems. bFGF therefore prevented retrograde degeneration of the thalamic neurons by a direct neurotrophic effect or indirect effect through astrocytes.
Keywordscerebral ischemia basic fibroblast growth factor in vitro hypoxia retrograde degeneration neurotrophic factor
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