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Ion Transport in HT29 Colonic Carcinoma Cells

  • Karl Kunzelmann
  • Monika Tilmann
  • Rainer Greger
Part of the Advances in Comparative and Environmental Physiology book series (COMPARATIVE, volume 16)

Abstract

The permanent cell line HT29 was established by Fogh and Trempe in 1975 from an adenocarcinoma of human colon (Fogh and Trempe 1975). In the following years, the cell line was examined and characterized with respect to many different biochemical and biophysical properties. HT29 cells, besides other colonic carcinoma cell lines such as T84 and CACO-2, serve as a very useful model in studying growth and differentiation of epithelial cells (Rousset 1986; Le Bivic et al. 1988; Hekmati et al. 1990). Moreover, the presence of a number of different hormone receptors was demonstrated in the membranes of HT29 cells. Second messenger systems activated by receptor binding were examined and are still under investigation (cf. Table 1). In all instances, the occupation of the receptor was linked to a change in the ion conductance properties of the HT29 cell. In terms of membrane transport proteins, in addition to the (Na+/K+)-ATPase, the presence of the following systems has been shown in HT29 cells: the Na+/H+ exchanger (Cantiello and Lanier 1989); the Na+/2Cl/K+ cotransporter (Kim et al. 1988); a K+ conductance (Wu et al. 1991); and a Cl conductance (Ziss et al. 1987; Lohrmann et al. 1991; Kunzelmann et al. 1992a). Because of their polar growth, which is developed under certain culture conditions, HT29 cells are also useful for studying electrolyte transport, and they are commonly looked at as a paradigm of the colonic crypt cell.

Keywords

Cystic Fibrosis HT29 Cell Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel Regulatory Volume Decrease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Karl Kunzelmann
  • Monika Tilmann
  • Rainer Greger
    • 1
  1. 1.Physiologisches InstitutAlbrecht-Ludwigs-UniversitätFreiburgGermany

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