Monitoring of an rDNA B. subtilis in environmental samples
For an industrial large-scale production of alpha amylase with an rDNA B. subtilis a case study was performed in the former G.D.R. from 1986–1990. Unintended releases of production strains are expected to occur in most cases of large-scale fermentations under safety level 1 conditions. Dominant release routes are via air (exhaust air from aerobic fermentation), biomass (after separation of the production strain from the extracellular enzymes) and waste water (Smalla et al. 1991). Our investigations were focussed on monitoring the extent and routes of release, characterization of microbial composition of bioprocessing waste, the fate of rDNA B. subtilis in sterile and nonsterile environmental media (potential recipient habitats such as soil, river water or waste water) and transfer of rDNA to indigenous microbes.
KeywordsProduction Strain Bacillus Licheniformis Most Probable Number Alpha Amylase Heavy Metal Resistance
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