Analysis of Transforming Sequences in Neoplastic Syrian Hamster Fetal Cells Induced by Diverse Chemical Carcinogens

  • V. Notario
  • N. Popescu
  • J. A. DiPaolo
Conference paper

Abstract

Neoplastic transformation occurs with chemical carcinogens that interact with DNA such as carcinogenic polycyclic hydrocarbons, inorganic metals, N-Acetoxy-florenylacetamide and its derivatives and alkylating agents (1). Transformation resulting in malignancy also occurs with non-mutagenic agents including SO 2 (sodium bisulfite). With chemicals alone or in combination with X-irradiation the incidence of transformed hamster colonies fits a one hit curve indicating that the phenomenon is inductive (2).

Keywords

Sucrose Hydrate Agarose Electrophoresis Adduct 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    DiPaoIo JA and Casto BC (1978) In vitro carcinogenesis with cells in early passage. Third Decennial Review Conference; Cell, Tissue, and Organ Culture. U.S. Government Printing Office, Washington, DC 20402.Google Scholar
  2. 2.
    Gart JJ, DiPaoIo JA and Donovan PJ. (1979) Mathematical models and the statistical analysis of cell transformation experiments. Cancer Res. 39:5069–5075Google Scholar
  3. 3.
    Higgins ITT (1971) Effects of sulfur oxides and particulates on health. Arch. Environ. Health, 22:584–590.Google Scholar
  4. 4.
    DiPaoIo JA, DeMarinis AJ and Doniger J (1981) Transformation of Syrian hamster embryo cells of sodium bisulfite, Cancer Lett., 12:203–208.CrossRefGoogle Scholar
  5. 5.
    Doniger J, O’Neil R and DiPaoIo JA (1982) Neoplastic transformation of Syrian hamster embryo cells by bisulfite is accompanied with a decrease in the number of functioning replicons. Carcinogenesis (Lond.), 3:27–32.CrossRefGoogle Scholar
  6. 6.
    Popescu NC and DiPaoIo JA (1988) Chromosome alterations in Syrian hamster cells transformed in vitro by sodium bisulfite, a nonclastogenic carcinogen. Cancer Res 48:7246–7251.Google Scholar
  7. 7.
    Wirth PJ, Doniger J. Thorgeirsson SS and DiPaoIo JA (1986) Altered polypeptide expression associated with Neoplastic transformation of Syrian hamster cells by bisulfite. Cancer Res 46:390–399.Google Scholar
  8. 8.
    Balmain A and Brown K (1988) Oncogene activation in chemical carcinogenesis. Adv. Cancer Res 51:147–182.CrossRefGoogle Scholar
  9. 9.
    Wigler M, Silverstein S, Lee LS, Pellicer A, Cheng Y and Axel R (1977) Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell 11:223–232CrossRefGoogle Scholar
  10. 10.
    Weinberg RA (1981) Use of transfection to analyze genetic information and malignant transformation. Biochim Biophys Acta 651:25–35.Google Scholar
  11. 11.
    Gilmer TM, Annab LA and Barrett JC (1988) Characterization of activated protooncogenes in chemically transformed Syrian hamster embryo cells. Mol Carcin 1:180–188.CrossRefGoogle Scholar
  12. 12.
    Furth ME, Davis LJ, Fleurdelys B and Scolnick EM (1982) Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular raş gene family. J Virol 43:294–304.Google Scholar
  13. 13.
    Cooper GM and Lane MA (1983) Cellular transforming genes and oncogenesis. Biochim Biophys Acta 738:9–20.Google Scholar
  14. 14.
    Notario V, Castro R, Flessate DM, Doniger J and DiPaoIo JA (1990) Frequent activation of non-ras transforming sequences in neoplastic Syrian hamster cells initiated with chemical carcinogens. Oncogene 5:1425–1430.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • V. Notario
    • 1
  • N. Popescu
    • 2
  • J. A. DiPaolo
    • 2
  1. 1.Department of Radiation MedicineGeorgetown University School of MedicineWashington, DCUSA
  2. 2.Laboratory of BiologyNCIBethesdaUSA

Personalised recommendations